Experiment: Control_ECRC62
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rbsA and rbsC are separated by 4 nucleotides rbsC and rbsB are separated by 24 nucleotides rbsB and rbsK are separated by 125 nucleotides
BNILDI_04645: rbsA - ribose ABC transporter ATP-binding protein RbsA, at 920,756 to 922,261
rbsA
BNILDI_04650: rbsC - ribose ABC transporter permease, at 922,266 to 923,231
rbsC
BNILDI_04655: rbsB - ribose ABC transporter substrate-binding protein RbsB, at 923,256 to 924,146
rbsB
BNILDI_04660: rbsK - ribokinase, at 924,272 to 925,201
rbsK
Position (kb) 923
924
925 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 922.402 kb on + strand, within rbsC at 922.403 kb on - strand, within rbsC at 922.492 kb on + strand, within rbsC at 922.532 kb on + strand, within rbsC at 922.533 kb on - strand, within rbsC at 922.533 kb on - strand, within rbsC at 922.653 kb on - strand, within rbsC at 922.661 kb on + strand, within rbsC at 922.662 kb on - strand, within rbsC at 922.673 kb on - strand, within rbsC at 922.695 kb on + strand, within rbsC at 922.696 kb on - strand, within rbsC at 922.698 kb on - strand, within rbsC at 922.707 kb on - strand, within rbsC at 922.827 kb on + strand, within rbsC at 922.827 kb on + strand, within rbsC at 922.827 kb on + strand, within rbsC at 922.828 kb on - strand, within rbsC at 922.864 kb on - strand, within rbsC at 922.864 kb on - strand, within rbsC at 922.864 kb on - strand, within rbsC at 922.864 kb on - strand, within rbsC at 922.935 kb on + strand, within rbsC at 923.113 kb on + strand, within rbsC at 923.233 kb on - strand at 923.239 kb on + strand at 923.256 kb on - strand at 923.300 kb on - strand at 923.363 kb on + strand, within rbsB at 923.543 kb on - strand, within rbsB at 923.546 kb on + strand, within rbsB at 923.673 kb on + strand, within rbsB at 923.673 kb on + strand, within rbsB at 923.732 kb on + strand, within rbsB at 923.732 kb on + strand, within rbsB at 923.733 kb on - strand, within rbsB at 923.793 kb on - strand, within rbsB at 923.845 kb on + strand, within rbsB at 923.846 kb on - strand, within rbsB at 923.887 kb on + strand, within rbsB at 924.016 kb on + strand, within rbsB at 924.108 kb on - strand at 924.138 kb on - strand at 924.144 kb on + strand at 924.144 kb on + strand at 924.145 kb on - strand at 924.151 kb on - strand at 924.161 kb on + strand at 924.161 kb on + strand at 924.162 kb on - strand at 924.179 kb on + strand at 924.179 kb on + strand at 924.180 kb on - strand at 924.183 kb on + strand at 924.183 kb on + strand at 924.183 kb on + strand at 924.184 kb on - strand at 924.184 kb on - strand at 924.184 kb on - strand at 924.184 kb on - strand at 924.206 kb on + strand at 924.207 kb on - strand at 924.210 kb on - strand at 924.210 kb on - strand at 924.211 kb on + strand at 924.239 kb on - strand at 924.310 kb on + strand at 924.329 kb on - strand at 924.347 kb on - strand at 924.512 kb on - strand, within rbsK at 924.520 kb on + strand, within rbsK at 924.524 kb on - strand, within rbsK at 924.664 kb on + strand, within rbsK at 924.684 kb on + strand, within rbsK at 924.687 kb on + strand, within rbsK at 924.687 kb on + strand, within rbsK at 924.748 kb on + strand, within rbsK at 924.821 kb on - strand, within rbsK at 924.823 kb on + strand, within rbsK at 924.824 kb on - strand, within rbsK at 924.937 kb on + strand, within rbsK at 924.937 kb on + strand, within rbsK at 924.937 kb on + strand, within rbsK at 924.937 kb on + strand, within rbsK at 924.938 kb on - strand, within rbsK at 925.136 kb on - strand at 925.136 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC62 remove 922,402 + rbsC BNILDI_04650 0.14 -1.9 922,403 - rbsC BNILDI_04650 0.14 +0.9 922,492 + rbsC BNILDI_04650 0.23 +0.2 922,532 + rbsC BNILDI_04650 0.28 -0.5 922,533 - rbsC BNILDI_04650 0.28 +0.8 922,533 - rbsC BNILDI_04650 0.28 +0.6 922,653 - rbsC BNILDI_04650 0.40 -0.3 922,661 + rbsC BNILDI_04650 0.41 -0.1 922,662 - rbsC BNILDI_04650 0.41 -0.1 922,673 - rbsC BNILDI_04650 0.42 -0.8 922,695 + rbsC BNILDI_04650 0.44 +1.5 922,696 - rbsC BNILDI_04650 0.45 +1.4 922,698 - rbsC BNILDI_04650 0.45 +1.5 922,707 - rbsC BNILDI_04650 0.46 -0.3 922,827 + rbsC BNILDI_04650 0.58 +0.6 922,827 + rbsC BNILDI_04650 0.58 -0.5 922,827 + rbsC BNILDI_04650 0.58 +0.2 922,828 - rbsC BNILDI_04650 0.58 -0.1 922,864 - rbsC BNILDI_04650 0.62 -0.1 922,864 - rbsC BNILDI_04650 0.62 +0.5 922,864 - rbsC BNILDI_04650 0.62 -0.1 922,864 - rbsC BNILDI_04650 0.62 -0.4 922,935 + rbsC BNILDI_04650 0.69 +0.2 923,113 + rbsC BNILDI_04650 0.88 -1.0 923,233 - -1.8 923,239 + +0.0 923,256 - +0.0 923,300 - -0.1 923,363 + rbsB BNILDI_04655 0.12 +0.0 923,543 - rbsB BNILDI_04655 0.32 -1.3 923,546 + rbsB BNILDI_04655 0.33 -0.3 923,673 + rbsB BNILDI_04655 0.47 -0.5 923,673 + rbsB BNILDI_04655 0.47 -1.2 923,732 + rbsB BNILDI_04655 0.53 +0.9 923,732 + rbsB BNILDI_04655 0.53 +1.6 923,733 - rbsB BNILDI_04655 0.54 -0.1 923,793 - rbsB BNILDI_04655 0.60 +0.0 923,845 + rbsB BNILDI_04655 0.66 -3.3 923,846 - rbsB BNILDI_04655 0.66 -2.8 923,887 + rbsB BNILDI_04655 0.71 -0.3 924,016 + rbsB BNILDI_04655 0.85 -1.3 924,108 - -1.7 924,138 - +0.1 924,144 + -0.1 924,144 + +1.5 924,145 - +1.9 924,151 - +0.1 924,161 + +3.1 924,161 + +2.2 924,162 - -0.4 924,179 + -0.8 924,179 + -3.1 924,180 - +0.7 924,183 + -0.2 924,183 + -0.3 924,183 + -0.0 924,184 - -0.3 924,184 - -0.2 924,184 - +3.3 924,184 - -0.6 924,206 + -0.0 924,207 - +0.3 924,210 - +0.2 924,210 - +0.7 924,211 + +0.4 924,239 - -0.3 924,310 + +1.8 924,329 - -0.1 924,347 - -0.1 924,512 - rbsK BNILDI_04660 0.26 +2.3 924,520 + rbsK BNILDI_04660 0.27 -3.0 924,524 - rbsK BNILDI_04660 0.27 -0.3 924,664 + rbsK BNILDI_04660 0.42 +0.4 924,684 + rbsK BNILDI_04660 0.44 +0.6 924,687 + rbsK BNILDI_04660 0.45 -1.4 924,687 + rbsK BNILDI_04660 0.45 +0.4 924,748 + rbsK BNILDI_04660 0.51 -2.8 924,821 - rbsK BNILDI_04660 0.59 +0.8 924,823 + rbsK BNILDI_04660 0.59 -0.1 924,824 - rbsK BNILDI_04660 0.59 -0.1 924,937 + rbsK BNILDI_04660 0.72 -1.7 924,937 + rbsK BNILDI_04660 0.72 +1.3 924,937 + rbsK BNILDI_04660 0.72 -0.7 924,937 + rbsK BNILDI_04660 0.72 -0.5 924,938 - rbsK BNILDI_04660 0.72 -0.2 925,136 - -1.4 925,136 - +0.2
Or see this region's nucleotide sequence