Experiment: Phage M13-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OHPLBJKB_04421 and OHPLBJKB_04422 are separated by 65 nucleotides OHPLBJKB_04422 and OHPLBJKB_04423 are separated by 56 nucleotides OHPLBJKB_04423 and OHPLBJKB_04424 are separated by 805 nucleotides OHPLBJKB_04424 and OHPLBJKB_04425 overlap by 4 nucleotides
OHPLBJKB_04421: OHPLBJKB_04421 - Nucleoid occlusion protein, at 72,611 to 74,569
_04421
OHPLBJKB_04422: OHPLBJKB_04422 - hypothetical protein, at 74,635 to 74,868
_04422
OHPLBJKB_04423: OHPLBJKB_04423 - Plasmid-derived single-stranded DNA-binding protein, at 74,925 to 75,464
_04423
OHPLBJKB_04424: OHPLBJKB_04424 - hypothetical protein, at 76,270 to 76,461
_04424
OHPLBJKB_04425: OHPLBJKB_04425 - hypothetical protein, at 76,458 to 76,880
_04425
Position (kb) 74
75
76 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 74.437 kb on + strand at 74.437 kb on + strand at 74.437 kb on + strand at 74.551 kb on + strand at 74.551 kb on + strand at 74.552 kb on - strand at 74.569 kb on + strand at 74.569 kb on + strand at 74.570 kb on - strand at 74.570 kb on - strand at 74.570 kb on - strand at 74.570 kb on - strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.574 kb on - strand at 74.574 kb on - strand at 74.574 kb on - strand at 74.574 kb on - strand at 74.576 kb on + strand at 74.576 kb on + strand at 74.576 kb on + strand at 74.577 kb on - strand at 74.594 kb on + strand at 74.595 kb on - strand at 74.637 kb on - strand at 74.640 kb on + strand at 74.640 kb on + strand at 74.641 kb on - strand at 74.641 kb on - strand at 74.641 kb on - strand at 74.641 kb on - strand at 74.641 kb on - strand at 74.707 kb on - strand, within OHPLBJKB_04422 at 74.727 kb on + strand, within OHPLBJKB_04422 at 74.727 kb on + strand, within OHPLBJKB_04422 at 74.848 kb on + strand at 74.848 kb on + strand at 74.849 kb on - strand at 75.002 kb on + strand, within OHPLBJKB_04423 at 75.002 kb on + strand, within OHPLBJKB_04423 at 75.002 kb on + strand, within OHPLBJKB_04423 at 75.003 kb on - strand, within OHPLBJKB_04423 at 75.126 kb on + strand, within OHPLBJKB_04423 at 75.126 kb on + strand, within OHPLBJKB_04423 at 75.127 kb on - strand, within OHPLBJKB_04423 at 75.155 kb on + strand, within OHPLBJKB_04423 at 75.229 kb on + strand, within OHPLBJKB_04423 at 75.230 kb on - strand, within OHPLBJKB_04423 at 75.397 kb on + strand, within OHPLBJKB_04423 at 75.398 kb on - strand, within OHPLBJKB_04423 at 75.398 kb on - strand, within OHPLBJKB_04423 at 75.429 kb on + strand at 75.697 kb on + strand at 76.268 kb on + strand at 76.269 kb on - strand at 76.298 kb on + strand, within OHPLBJKB_04424 at 76.298 kb on + strand, within OHPLBJKB_04424 at 76.298 kb on + strand, within OHPLBJKB_04424 at 76.299 kb on - strand, within OHPLBJKB_04424 at 76.299 kb on - strand, within OHPLBJKB_04424 at 76.299 kb on - strand, within OHPLBJKB_04424 at 76.421 kb on + strand, within OHPLBJKB_04424 at 76.455 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phage M13-1 remove 74,437 + -1.1 74,437 + -1.4 74,437 + -2.1 74,551 + -1.0 74,551 + -0.8 74,552 - -3.2 74,569 + +2.2 74,569 + +0.4 74,570 - +0.3 74,570 - +0.4 74,570 - +0.7 74,570 - -0.7 74,573 + +0.8 74,573 + -0.7 74,573 + +0.4 74,573 + -0.7 74,573 + -0.6 74,573 + -1.4 74,573 + -3.2 74,573 + +0.6 74,574 - -1.1 74,574 - -1.1 74,574 - +0.0 74,574 - +1.4 74,576 + -0.1 74,576 + +1.4 74,576 + +0.2 74,577 - -2.3 74,594 + +0.5 74,595 - -2.4 74,637 - -1.4 74,640 + +0.4 74,640 + +0.6 74,641 - +0.2 74,641 - +0.5 74,641 - -3.5 74,641 - -0.7 74,641 - +0.7 74,707 - OHPLBJKB_04422 0.31 -1.6 74,727 + OHPLBJKB_04422 0.39 -0.7 74,727 + OHPLBJKB_04422 0.39 -0.7 74,848 + +0.1 74,848 + -0.2 74,849 - -0.1 75,002 + OHPLBJKB_04423 0.14 -0.3 75,002 + OHPLBJKB_04423 0.14 +0.1 75,002 + OHPLBJKB_04423 0.14 -1.6 75,003 - OHPLBJKB_04423 0.14 -0.3 75,126 + OHPLBJKB_04423 0.37 -0.8 75,126 + OHPLBJKB_04423 0.37 +1.0 75,127 - OHPLBJKB_04423 0.37 +0.3 75,155 + OHPLBJKB_04423 0.43 +0.6 75,229 + OHPLBJKB_04423 0.56 -0.7 75,230 - OHPLBJKB_04423 0.56 -0.9 75,397 + OHPLBJKB_04423 0.87 -0.7 75,398 - OHPLBJKB_04423 0.88 +0.4 75,398 - OHPLBJKB_04423 0.88 -1.1 75,429 + -0.5 75,697 + -0.4 76,268 + -0.5 76,269 - +1.3 76,298 + OHPLBJKB_04424 0.15 +0.9 76,298 + OHPLBJKB_04424 0.15 +1.4 76,298 + OHPLBJKB_04424 0.15 +0.4 76,299 - OHPLBJKB_04424 0.15 -1.0 76,299 - OHPLBJKB_04424 0.15 -0.6 76,299 - OHPLBJKB_04424 0.15 +0.3 76,421 + OHPLBJKB_04424 0.79 +0.4 76,455 + +0.4
Or see this region's nucleotide sequence