Experiment: Bas05
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgm and seqA are separated by 25 nucleotides seqA and ybfF are separated by 184 nucleotides ybfF and ybfE are separated by 70 nucleotides ybfE and fldA are separated by 139 nucleotides
BNILDI_21485: pgm - phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent), at 4,324,160 to 4,325,800
pgm
BNILDI_21490: seqA - replication initiation negative regulator SeqA, at 4,325,826 to 4,326,371
seqA
BNILDI_21495: ybfF - esterase, at 4,326,556 to 4,327,320
ybfF
BNILDI_21500: ybfE - LexA regulated protein, at 4,327,391 to 4,327,753
ybfE
BNILDI_21505: fldA - flavodoxin FldA, at 4,327,893 to 4,328,423
fldA
Position (kb)
4326
4327
4328 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4325.557 kb on - strand, within pgm at 4325.721 kb on + strand at 4325.721 kb on + strand at 4325.827 kb on + strand at 4326.076 kb on + strand, within seqA at 4326.078 kb on + strand, within seqA at 4326.131 kb on - strand, within seqA at 4326.279 kb on - strand, within seqA at 4326.279 kb on - strand, within seqA at 4326.334 kb on + strand at 4326.334 kb on + strand at 4326.335 kb on - strand at 4326.400 kb on + strand at 4326.401 kb on - strand at 4326.417 kb on - strand at 4326.417 kb on - strand at 4326.418 kb on + strand at 4326.418 kb on + strand at 4326.418 kb on + strand at 4326.425 kb on - strand at 4326.483 kb on - strand at 4326.516 kb on + strand at 4326.517 kb on - strand at 4326.517 kb on - strand at 4326.517 kb on - strand at 4326.519 kb on + strand at 4326.520 kb on - strand at 4326.688 kb on - strand, within ybfF at 4326.703 kb on - strand, within ybfF at 4326.775 kb on - strand, within ybfF at 4326.849 kb on + strand, within ybfF at 4326.849 kb on + strand, within ybfF at 4326.917 kb on + strand, within ybfF at 4326.918 kb on - strand, within ybfF at 4326.918 kb on - strand, within ybfF at 4327.015 kb on - strand, within ybfF at 4327.107 kb on + strand, within ybfF at 4327.108 kb on - strand, within ybfF at 4327.177 kb on + strand, within ybfF at 4327.287 kb on - strand at 4327.394 kb on + strand at 4327.394 kb on + strand at 4327.394 kb on + strand at 4327.394 kb on + strand at 4327.395 kb on - strand at 4327.395 kb on - strand at 4327.491 kb on + strand, within ybfE at 4327.491 kb on + strand, within ybfE at 4327.492 kb on - strand, within ybfE at 4327.565 kb on - strand, within ybfE at 4327.599 kb on + strand, within ybfE at 4327.599 kb on + strand, within ybfE at 4327.600 kb on - strand, within ybfE at 4327.600 kb on - strand, within ybfE at 4327.678 kb on + strand, within ybfE at 4327.735 kb on + strand at 4327.770 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas05 remove 4,325,557 - pgm BNILDI_21485 0.85 +0.3 4,325,721 + -1.6 4,325,721 + +2.3 4,325,827 + -1.9 4,326,076 + seqA BNILDI_21490 0.46 -1.1 4,326,078 + seqA BNILDI_21490 0.46 -0.7 4,326,131 - seqA BNILDI_21490 0.56 +3.5 4,326,279 - seqA BNILDI_21490 0.83 -1.9 4,326,279 - seqA BNILDI_21490 0.83 -0.8 4,326,334 + -2.0 4,326,334 + -1.6 4,326,335 - -1.4 4,326,400 + -0.0 4,326,401 - -0.9 4,326,417 - +0.1 4,326,417 - -0.4 4,326,418 + -0.4 4,326,418 + -1.9 4,326,418 + -0.7 4,326,425 - -0.0 4,326,483 - -0.6 4,326,516 + -0.1 4,326,517 - -0.7 4,326,517 - -0.2 4,326,517 - -0.4 4,326,519 + +3.3 4,326,520 - +0.3 4,326,688 - ybfF BNILDI_21495 0.17 -1.1 4,326,703 - ybfF BNILDI_21495 0.19 -0.8 4,326,775 - ybfF BNILDI_21495 0.29 -1.6 4,326,849 + ybfF BNILDI_21495 0.38 -2.7 4,326,849 + ybfF BNILDI_21495 0.38 -1.3 4,326,917 + ybfF BNILDI_21495 0.47 +0.3 4,326,918 - ybfF BNILDI_21495 0.47 -0.3 4,326,918 - ybfF BNILDI_21495 0.47 +0.1 4,327,015 - ybfF BNILDI_21495 0.60 -0.5 4,327,107 + ybfF BNILDI_21495 0.72 -1.0 4,327,108 - ybfF BNILDI_21495 0.72 -1.1 4,327,177 + ybfF BNILDI_21495 0.81 -0.5 4,327,287 - +0.2 4,327,394 + -0.0 4,327,394 + -0.6 4,327,394 + -1.0 4,327,394 + +0.1 4,327,395 - +0.9 4,327,395 - +0.1 4,327,491 + ybfE BNILDI_21500 0.28 -0.6 4,327,491 + ybfE BNILDI_21500 0.28 +0.7 4,327,492 - ybfE BNILDI_21500 0.28 +1.2 4,327,565 - ybfE BNILDI_21500 0.48 -0.5 4,327,599 + ybfE BNILDI_21500 0.57 -0.3 4,327,599 + ybfE BNILDI_21500 0.57 +0.3 4,327,600 - ybfE BNILDI_21500 0.58 +1.0 4,327,600 - ybfE BNILDI_21500 0.58 -2.3 4,327,678 + ybfE BNILDI_21500 0.79 +0.0 4,327,735 + -0.5 4,327,770 - -0.9
Or see this region's nucleotide sequence