Strain Fitness in Escherichia coli ECRC62 around BNILDI_21415

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyhhI and ybfQ are separated by 218 nucleotidesybfQ and ybfC are separated by 225 nucleotidesybfC and rhsA overlap by 4 nucleotides BNILDI_21410: yhhI - ISAs1 family transposase, at 4,306,716 to 4,307,384 yhhI BNILDI_21415: ybfQ - Inactive transposase YbfQ, at 4,307,603 to 4,307,857 ybfQ BNILDI_21420: ybfC - Uncharacterized protein YbfC, at 4,308,083 to 4,308,652 ybfC BNILDI_21425: rhsA - Uncharacterized conserved protein RhaS, contains 28 RHS repeats, at 4,308,649 to 4,310,082 rhsA Position (kb) 4307 4308Strain fitness (log2 ratio) -3 -2 -1 0 1at 4306.631 kb on - strandat 4306.631 kb on - strandat 4306.641 kb on + strandat 4306.705 kb on - strandat 4306.907 kb on - strand, within yhhIat 4306.986 kb on - strand, within yhhIat 4307.656 kb on + strand, within ybfQat 4307.758 kb on + strand, within ybfQat 4308.220 kb on + strand, within ybfCat 4308.281 kb on + strand, within ybfCat 4308.713 kb on - strandat 4308.777 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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4,306,631 - +0.5
4,306,631 - -0.2
4,306,641 + -0.3
4,306,705 - -0.4
4,306,907 - yhhI BNILDI_21410 0.29 +0.3
4,306,986 - yhhI BNILDI_21410 0.40 +0.3
4,307,656 + ybfQ BNILDI_21415 0.21 -1.0
4,307,758 + ybfQ BNILDI_21415 0.61 -2.8
4,308,220 + ybfC BNILDI_21420 0.24 +1.0
4,308,281 + ybfC BNILDI_21420 0.35 +0.5
4,308,713 - +0.3
4,308,777 + +0.4

Or see this region's nucleotide sequence