Strain Fitness in Escherichia coli ECRC62 around BNILDI_21300

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmngR and sucD are separated by 103 nucleotidessucD and sucC overlap by 1 nucleotidessucC and odhB are separated by 93 nucleotides BNILDI_21290: mngR - mannosyl-D-glycerate transport/metabolism system transcriptional repressor MngR, at 4,280,076 to 4,280,798 mngR BNILDI_21295: sucD - succinate--CoA ligase subunit alpha, at 4,280,902 to 4,281,771 sucD BNILDI_21300: sucC - ADP-forming succinate--CoA ligase subunit beta, at 4,281,771 to 4,282,937 sucC BNILDI_21305: odhB - 2-oxoglutarate dehydrogenase complex dihydrolipoyllysine-residue succinyltransferase, at 4,283,031 to 4,284,248 odhB Position (kb) 4281 4282 4283Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4280.817 kb on + strandat 4280.817 kb on + strandat 4280.884 kb on - strandat 4280.884 kb on - strandat 4280.899 kb on + strandat 4280.904 kb on - strandat 4281.039 kb on + strand, within sucDat 4281.074 kb on + strand, within sucDat 4281.075 kb on - strand, within sucDat 4281.110 kb on + strand, within sucDat 4281.157 kb on + strand, within sucDat 4281.417 kb on + strand, within sucDat 4281.418 kb on - strand, within sucDat 4281.516 kb on + strand, within sucDat 4281.553 kb on + strand, within sucDat 4281.557 kb on + strand, within sucDat 4281.557 kb on + strand, within sucDat 4281.557 kb on + strand, within sucDat 4281.557 kb on + strand, within sucDat 4281.558 kb on - strand, within sucDat 4281.558 kb on - strand, within sucDat 4281.558 kb on - strand, within sucDat 4281.558 kb on - strand, within sucDat 4281.673 kb on - strand, within sucDat 4281.673 kb on - strand, within sucDat 4281.772 kb on + strandat 4281.772 kb on + strandat 4282.154 kb on + strand, within sucCat 4282.154 kb on + strand, within sucCat 4282.154 kb on + strand, within sucCat 4282.155 kb on - strand, within sucCat 4282.155 kb on - strand, within sucCat 4282.155 kb on - strand, within sucCat 4282.184 kb on - strand, within sucCat 4282.590 kb on - strand, within sucCat 4282.612 kb on + strand, within sucCat 4282.688 kb on - strand, within sucCat 4282.860 kb on - strandat 4282.870 kb on + strandat 4282.929 kb on + strandat 4282.930 kb on - strandat 4282.980 kb on - strandat 4283.006 kb on + strandat 4283.007 kb on - strandat 4283.024 kb on + strandat 4283.033 kb on - strandat 4283.247 kb on - strand, within odhBat 4283.453 kb on - strand, within odhBat 4283.591 kb on - strand, within odhB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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4,280,817 + -0.4
4,280,817 + +0.4
4,280,884 - -0.1
4,280,884 - -0.3
4,280,899 + -0.2
4,280,904 - +1.0
4,281,039 + sucD BNILDI_21295 0.16 +1.2
4,281,074 + sucD BNILDI_21295 0.20 +3.3
4,281,075 - sucD BNILDI_21295 0.20 -0.4
4,281,110 + sucD BNILDI_21295 0.24 -0.8
4,281,157 + sucD BNILDI_21295 0.29 -0.0
4,281,417 + sucD BNILDI_21295 0.59 -0.3
4,281,418 - sucD BNILDI_21295 0.59 -2.1
4,281,516 + sucD BNILDI_21295 0.71 -0.7
4,281,553 + sucD BNILDI_21295 0.75 -1.8
4,281,557 + sucD BNILDI_21295 0.75 -2.0
4,281,557 + sucD BNILDI_21295 0.75 -0.5
4,281,557 + sucD BNILDI_21295 0.75 +0.4
4,281,557 + sucD BNILDI_21295 0.75 -0.8
4,281,558 - sucD BNILDI_21295 0.75 -0.3
4,281,558 - sucD BNILDI_21295 0.75 -2.0
4,281,558 - sucD BNILDI_21295 0.75 +0.0
4,281,558 - sucD BNILDI_21295 0.75 -0.2
4,281,673 - sucD BNILDI_21295 0.89 +1.2
4,281,673 - sucD BNILDI_21295 0.89 +0.7
4,281,772 + +0.3
4,281,772 + -0.8
4,282,154 + sucC BNILDI_21300 0.33 -1.4
4,282,154 + sucC BNILDI_21300 0.33 -0.7
4,282,154 + sucC BNILDI_21300 0.33 -1.0
4,282,155 - sucC BNILDI_21300 0.33 -1.6
4,282,155 - sucC BNILDI_21300 0.33 +0.2
4,282,155 - sucC BNILDI_21300 0.33 -1.3
4,282,184 - sucC BNILDI_21300 0.35 -0.0
4,282,590 - sucC BNILDI_21300 0.70 -0.7
4,282,612 + sucC BNILDI_21300 0.72 -1.7
4,282,688 - sucC BNILDI_21300 0.79 +0.2
4,282,860 - +1.8
4,282,870 + -0.0
4,282,929 + +0.2
4,282,930 - +1.2
4,282,980 - -0.9
4,283,006 + -0.3
4,283,007 - -0.2
4,283,024 + +0.2
4,283,033 - +1.2
4,283,247 - odhB BNILDI_21305 0.18 -0.2
4,283,453 - odhB BNILDI_21305 0.35 +1.3
4,283,591 - odhB BNILDI_21305 0.46 +2.3

Or see this region's nucleotide sequence