Experiment: Bas05
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepN and pncB are separated by 265 nucleotides pncB and asnS are separated by 168 nucleotides
BNILDI_20175: pepN - aminopeptidase N, at 4,048,440 to 4,051,052
pepN
BNILDI_20180: pncB - nicotinate phosphoribosyltransferase, at 4,051,318 to 4,052,520
pncB
BNILDI_20185: asnS - asparagine--tRNA ligase, at 4,052,689 to 4,054,089
asnS
Position (kb)
4051
4052
4053 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4050.320 kb on - strand, within pepN at 4050.413 kb on - strand, within pepN at 4050.450 kb on - strand, within pepN at 4050.465 kb on + strand, within pepN at 4050.465 kb on + strand, within pepN at 4050.598 kb on + strand, within pepN at 4050.661 kb on + strand, within pepN at 4050.662 kb on - strand, within pepN at 4050.664 kb on + strand, within pepN at 4050.668 kb on + strand, within pepN at 4050.668 kb on + strand, within pepN at 4050.669 kb on - strand, within pepN at 4050.669 kb on - strand, within pepN at 4050.669 kb on - strand, within pepN at 4050.760 kb on - strand, within pepN at 4050.776 kb on + strand, within pepN at 4050.854 kb on + strand at 4050.855 kb on - strand at 4050.856 kb on + strand at 4050.857 kb on + strand at 4050.993 kb on - strand at 4051.052 kb on + strand at 4051.056 kb on + strand at 4051.056 kb on + strand at 4051.073 kb on + strand at 4051.073 kb on + strand at 4051.074 kb on - strand at 4051.076 kb on - strand at 4051.108 kb on - strand at 4051.168 kb on + strand at 4051.169 kb on - strand at 4051.176 kb on - strand at 4051.210 kb on + strand at 4051.211 kb on - strand at 4051.232 kb on - strand at 4051.232 kb on - strand at 4051.249 kb on - strand at 4051.249 kb on - strand at 4051.275 kb on + strand at 4051.317 kb on + strand at 4051.318 kb on - strand at 4051.370 kb on - strand at 4051.370 kb on - strand at 4051.371 kb on + strand at 4051.372 kb on - strand at 4051.380 kb on + strand at 4051.406 kb on - strand at 4051.408 kb on + strand at 4051.408 kb on + strand at 4051.461 kb on + strand, within pncB at 4051.466 kb on - strand, within pncB at 4051.466 kb on - strand, within pncB at 4051.476 kb on + strand, within pncB at 4051.477 kb on - strand, within pncB at 4051.529 kb on - strand, within pncB at 4051.529 kb on - strand, within pncB at 4051.572 kb on + strand, within pncB at 4051.572 kb on + strand, within pncB at 4051.812 kb on + strand, within pncB at 4051.813 kb on - strand, within pncB at 4051.869 kb on - strand, within pncB at 4051.869 kb on - strand, within pncB at 4051.927 kb on + strand, within pncB at 4051.928 kb on - strand, within pncB at 4051.928 kb on - strand, within pncB at 4052.015 kb on + strand, within pncB at 4052.209 kb on + strand, within pncB at 4052.239 kb on - strand, within pncB at 4052.270 kb on + strand, within pncB at 4052.291 kb on - strand, within pncB at 4052.318 kb on + strand, within pncB at 4052.363 kb on + strand, within pncB at 4052.377 kb on - strand, within pncB at 4052.445 kb on + strand at 4052.518 kb on + strand at 4052.623 kb on + strand at 4052.623 kb on + strand at 4052.623 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas05 remove 4,050,320 - pepN BNILDI_20175 0.72 -0.1 4,050,413 - pepN BNILDI_20175 0.76 -0.4 4,050,450 - pepN BNILDI_20175 0.77 +0.1 4,050,465 + pepN BNILDI_20175 0.77 +0.1 4,050,465 + pepN BNILDI_20175 0.77 +0.2 4,050,598 + pepN BNILDI_20175 0.83 +1.5 4,050,661 + pepN BNILDI_20175 0.85 +0.4 4,050,662 - pepN BNILDI_20175 0.85 +0.6 4,050,664 + pepN BNILDI_20175 0.85 -0.5 4,050,668 + pepN BNILDI_20175 0.85 -1.1 4,050,668 + pepN BNILDI_20175 0.85 -0.8 4,050,669 - pepN BNILDI_20175 0.85 +0.1 4,050,669 - pepN BNILDI_20175 0.85 -0.0 4,050,669 - pepN BNILDI_20175 0.85 -0.9 4,050,760 - pepN BNILDI_20175 0.89 -0.1 4,050,776 + pepN BNILDI_20175 0.89 -0.4 4,050,854 + -1.3 4,050,855 - +0.1 4,050,856 + -0.7 4,050,857 + -0.8 4,050,993 - -0.6 4,051,052 + -0.1 4,051,056 + -2.3 4,051,056 + +1.5 4,051,073 + -0.6 4,051,073 + -0.0 4,051,074 - -2.0 4,051,076 - +1.5 4,051,108 - -0.6 4,051,168 + +0.2 4,051,169 - +0.0 4,051,176 - +0.3 4,051,210 + -0.2 4,051,211 - -0.3 4,051,232 - +0.4 4,051,232 - -0.0 4,051,249 - -0.6 4,051,249 - +0.1 4,051,275 + +2.1 4,051,317 + +0.6 4,051,318 - +0.9 4,051,370 - +0.3 4,051,370 - +0.7 4,051,371 + +1.1 4,051,372 - -0.5 4,051,380 + -0.4 4,051,406 - +0.8 4,051,408 + -0.5 4,051,408 + -0.8 4,051,461 + pncB BNILDI_20180 0.12 -1.7 4,051,466 - pncB BNILDI_20180 0.12 -1.8 4,051,466 - pncB BNILDI_20180 0.12 -1.6 4,051,476 + pncB BNILDI_20180 0.13 -1.7 4,051,477 - pncB BNILDI_20180 0.13 -0.8 4,051,529 - pncB BNILDI_20180 0.18 -0.4 4,051,529 - pncB BNILDI_20180 0.18 +1.4 4,051,572 + pncB BNILDI_20180 0.21 -2.8 4,051,572 + pncB BNILDI_20180 0.21 -2.3 4,051,812 + pncB BNILDI_20180 0.41 +0.1 4,051,813 - pncB BNILDI_20180 0.41 +0.3 4,051,869 - pncB BNILDI_20180 0.46 +0.4 4,051,869 - pncB BNILDI_20180 0.46 +0.4 4,051,927 + pncB BNILDI_20180 0.51 +0.4 4,051,928 - pncB BNILDI_20180 0.51 -0.4 4,051,928 - pncB BNILDI_20180 0.51 +0.3 4,052,015 + pncB BNILDI_20180 0.58 -0.6 4,052,209 + pncB BNILDI_20180 0.74 -1.0 4,052,239 - pncB BNILDI_20180 0.77 +3.3 4,052,270 + pncB BNILDI_20180 0.79 +0.5 4,052,291 - pncB BNILDI_20180 0.81 -0.0 4,052,318 + pncB BNILDI_20180 0.83 -0.3 4,052,363 + pncB BNILDI_20180 0.87 -0.6 4,052,377 - pncB BNILDI_20180 0.88 -0.6 4,052,445 + +0.4 4,052,518 + -0.9 4,052,623 + -1.1 4,052,623 + -0.7 4,052,623 + -2.7
Or see this region's nucleotide sequence