Strain Fitness in Escherichia coli ECRC62 around BNILDI_19980

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfabA and rmf are separated by 69 nucleotidesrmf and pqiC are separated by 255 nucleotidespqiC and pqiB overlap by 4 nucleotides BNILDI_19970: fabA - bifunctional 3-hydroxydecanoyl-ACP dehydratase/trans-2-decenoyl-ACP isomerase, at 4,007,633 to 4,008,259 fabA BNILDI_19975: rmf - ribosome modulation factor, at 4,008,329 to 4,008,496 rmf BNILDI_19980: pqiC - membrane integrity-associated transporter subunit PqiC, at 4,008,752 to 4,009,315 pqiC BNILDI_19985: pqiB - intermembrane transport protein PqiB, at 4,009,312 to 4,010,952 pqiB Position (kb) 4008 4009 4010Strain fitness (log2 ratio) -1 0 1at 4008.448 kb on - strand, within rmfat 4008.544 kb on + strandat 4008.547 kb on + strandat 4008.584 kb on + strandat 4008.750 kb on - strandat 4008.753 kb on + strandat 4008.754 kb on - strandat 4008.767 kb on + strandat 4009.126 kb on - strand, within pqiCat 4009.150 kb on - strand, within pqiCat 4009.150 kb on - strand, within pqiCat 4009.233 kb on - strand, within pqiCat 4009.242 kb on + strand, within pqiCat 4009.460 kb on + strandat 4009.461 kb on - strandat 4009.461 kb on - strandat 4009.750 kb on - strand, within pqiBat 4009.750 kb on - strand, within pqiBat 4009.750 kb on - strand, within pqiBat 4009.806 kb on - strand, within pqiBat 4009.950 kb on + strand, within pqiBat 4009.966 kb on - strand, within pqiB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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4,008,448 - rmf BNILDI_19975 0.71 +0.4
4,008,544 + -1.3
4,008,547 + +0.1
4,008,584 + -0.6
4,008,750 - +0.2
4,008,753 + +0.2
4,008,754 - -1.7
4,008,767 + -1.1
4,009,126 - pqiC BNILDI_19980 0.66 -0.0
4,009,150 - pqiC BNILDI_19980 0.71 -1.0
4,009,150 - pqiC BNILDI_19980 0.71 -1.4
4,009,233 - pqiC BNILDI_19980 0.85 -0.4
4,009,242 + pqiC BNILDI_19980 0.87 -0.6
4,009,460 + -1.0
4,009,461 - -0.2
4,009,461 - -0.7
4,009,750 - pqiB BNILDI_19985 0.27 +0.5
4,009,750 - pqiB BNILDI_19985 0.27 -0.3
4,009,750 - pqiB BNILDI_19985 0.27 -0.7
4,009,806 - pqiB BNILDI_19985 0.30 -1.2
4,009,950 + pqiB BNILDI_19985 0.39 -0.3
4,009,966 - pqiB BNILDI_19985 0.40 -0.8

Or see this region's nucleotide sequence