Strain Fitness in Escherichia coli ECRC62 around BNILDI_18545

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntclsA and cls are separated by 34 nucleotidescls and oppF are separated by 52 nucleotidesoppF and oppD overlap by 4 nucleotides BNILDI_18535: clsA - cardiolipin synthase ClsA, at 3,732,148 to 3,733,608 clsA BNILDI_18540: cls - cardiolipin synthase, at 3,733,643 to 3,733,972 cls BNILDI_18545: oppF - murein tripeptide/oligopeptide ABC transporter ATP-binding protein OppF, at 3,734,025 to 3,735,029 oppF BNILDI_18550: oppD - murein tripeptide/oligopeptide ABC transporter ATP-binding protein OppD, at 3,735,026 to 3,736,039 oppD Position (kb) 3734 3735 3736Strain fitness (log2 ratio) -2 -1 0 1 2at 3733.035 kb on + strand, within clsAat 3733.035 kb on + strand, within clsAat 3733.035 kb on + strand, within clsAat 3733.038 kb on + strand, within clsAat 3733.243 kb on - strand, within clsAat 3733.359 kb on + strand, within clsAat 3733.416 kb on + strand, within clsAat 3733.422 kb on + strand, within clsAat 3733.627 kb on + strandat 3733.643 kb on - strandat 3733.656 kb on + strandat 3733.657 kb on - strandat 3733.974 kb on + strandat 3734.167 kb on - strand, within oppFat 3734.251 kb on + strand, within oppFat 3734.260 kb on - strand, within oppFat 3734.284 kb on + strand, within oppFat 3734.285 kb on - strand, within oppFat 3734.314 kb on + strand, within oppFat 3734.314 kb on + strand, within oppFat 3734.346 kb on + strand, within oppFat 3734.513 kb on + strand, within oppFat 3734.638 kb on + strand, within oppFat 3734.643 kb on - strand, within oppFat 3734.678 kb on + strand, within oppFat 3734.843 kb on + strand, within oppFat 3734.871 kb on + strand, within oppFat 3734.871 kb on + strand, within oppFat 3735.049 kb on + strandat 3735.249 kb on + strand, within oppDat 3735.267 kb on + strand, within oppDat 3735.267 kb on + strand, within oppDat 3735.312 kb on + strand, within oppDat 3735.313 kb on - strand, within oppDat 3735.407 kb on + strand, within oppDat 3735.474 kb on - strand, within oppDat 3735.489 kb on - strand, within oppDat 3735.638 kb on - strand, within oppDat 3735.638 kb on - strand, within oppDat 3735.638 kb on - strand, within oppDat 3735.638 kb on - strand, within oppDat 3735.679 kb on + strand, within oppDat 3735.681 kb on + strand, within oppDat 3735.682 kb on - strand, within oppDat 3735.743 kb on - strand, within oppDat 3735.805 kb on + strand, within oppDat 3735.876 kb on - strand, within oppDat 3735.903 kb on - strand, within oppDat 3735.953 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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3,733,035 + clsA BNILDI_18535 0.61 +0.1
3,733,035 + clsA BNILDI_18535 0.61 +0.3
3,733,035 + clsA BNILDI_18535 0.61 -1.4
3,733,038 + clsA BNILDI_18535 0.61 -0.5
3,733,243 - clsA BNILDI_18535 0.75 +0.1
3,733,359 + clsA BNILDI_18535 0.83 +0.0
3,733,416 + clsA BNILDI_18535 0.87 -0.7
3,733,422 + clsA BNILDI_18535 0.87 -1.1
3,733,627 + -0.0
3,733,643 - -0.1
3,733,656 + +0.6
3,733,657 - +1.1
3,733,974 + -0.8
3,734,167 - oppF BNILDI_18545 0.14 +0.3
3,734,251 + oppF BNILDI_18545 0.22 -0.6
3,734,260 - oppF BNILDI_18545 0.23 -0.7
3,734,284 + oppF BNILDI_18545 0.26 -1.0
3,734,285 - oppF BNILDI_18545 0.26 -0.5
3,734,314 + oppF BNILDI_18545 0.29 +0.2
3,734,314 + oppF BNILDI_18545 0.29 +0.5
3,734,346 + oppF BNILDI_18545 0.32 +1.7
3,734,513 + oppF BNILDI_18545 0.49 -0.1
3,734,638 + oppF BNILDI_18545 0.61 -0.2
3,734,643 - oppF BNILDI_18545 0.61 +0.1
3,734,678 + oppF BNILDI_18545 0.65 -0.8
3,734,843 + oppF BNILDI_18545 0.81 +0.7
3,734,871 + oppF BNILDI_18545 0.84 -0.7
3,734,871 + oppF BNILDI_18545 0.84 -2.1
3,735,049 + -0.3
3,735,249 + oppD BNILDI_18550 0.22 -0.2
3,735,267 + oppD BNILDI_18550 0.24 -0.2
3,735,267 + oppD BNILDI_18550 0.24 +0.5
3,735,312 + oppD BNILDI_18550 0.28 +0.2
3,735,313 - oppD BNILDI_18550 0.28 -0.3
3,735,407 + oppD BNILDI_18550 0.38 +1.0
3,735,474 - oppD BNILDI_18550 0.44 -0.8
3,735,489 - oppD BNILDI_18550 0.46 +0.3
3,735,638 - oppD BNILDI_18550 0.60 +0.0
3,735,638 - oppD BNILDI_18550 0.60 -0.5
3,735,638 - oppD BNILDI_18550 0.60 +0.3
3,735,638 - oppD BNILDI_18550 0.60 +2.3
3,735,679 + oppD BNILDI_18550 0.64 +1.4
3,735,681 + oppD BNILDI_18550 0.65 -0.7
3,735,682 - oppD BNILDI_18550 0.65 +0.1
3,735,743 - oppD BNILDI_18550 0.71 +0.3
3,735,805 + oppD BNILDI_18550 0.77 -0.2
3,735,876 - oppD BNILDI_18550 0.84 -1.7
3,735,903 - oppD BNILDI_18550 0.86 +0.5
3,735,953 - -0.4

Or see this region's nucleotide sequence