Experiment: Bas05
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pheT and BNILDI_16405 are separated by 4 nucleotides BNILDI_16405 and BNILDI_16410 are separated by 305 nucleotides BNILDI_16410 and BNILDI_16415 are separated by 340 nucleotides
BNILDI_16400: pheT - phenylalanine--tRNA ligase subunit beta, at 3,283,871 to 3,286,258
pheT
BNILDI_16405: BNILDI_16405 - Integration host factor subunit alpha, at 3,286,263 to 3,286,562
_16405
BNILDI_16410: BNILDI_16410 - hok/gef family protein, at 3,286,868 to 3,287,008
_16410
BNILDI_16415: BNILDI_16415 - Baseplate assembly protein J, at 3,287,349 to 3,287,657
_16415
Position (kb)
3286
3287 Strain fitness (log2 ratio)
-1
0
1 at 3286.412 kb on + strand, within BNILDI_16405 at 3286.481 kb on + strand, within BNILDI_16405 at 3286.691 kb on + strand at 3286.711 kb on - strand at 3286.711 kb on - strand at 3286.712 kb on + strand at 3286.713 kb on - strand at 3286.801 kb on + strand at 3286.876 kb on + strand at 3286.877 kb on - strand at 3286.877 kb on - strand at 3286.877 kb on - strand at 3286.883 kb on + strand, within BNILDI_16410 at 3286.884 kb on - strand, within BNILDI_16410 at 3286.922 kb on + strand, within BNILDI_16410 at 3287.009 kb on - strand at 3287.161 kb on - strand at 3287.212 kb on - strand at 3287.403 kb on + strand, within BNILDI_16415 at 3287.440 kb on - strand, within BNILDI_16415
Per-strain Table
Position Strand Gene LocusTag Fraction Bas05 remove 3,286,412 + BNILDI_16405 0.50 -1.4 3,286,481 + BNILDI_16405 0.73 -0.5 3,286,691 + +0.5 3,286,711 - +0.0 3,286,711 - +0.5 3,286,712 + +0.4 3,286,713 - -0.2 3,286,801 + -0.7 3,286,876 + -1.2 3,286,877 - +1.4 3,286,877 - -1.6 3,286,877 - -0.6 3,286,883 + BNILDI_16410 0.11 -0.6 3,286,884 - BNILDI_16410 0.11 -0.3 3,286,922 + BNILDI_16410 0.38 -0.1 3,287,009 - -0.9 3,287,161 - +1.2 3,287,212 - +0.0 3,287,403 + BNILDI_16415 0.17 +1.4 3,287,440 - BNILDI_16415 0.29 -0.3
Or see this region's nucleotide sequence