Strain Fitness in Escherichia coli ECRC62 around BNILDI_16405

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpheT and BNILDI_16405 are separated by 4 nucleotidesBNILDI_16405 and BNILDI_16410 are separated by 305 nucleotidesBNILDI_16410 and BNILDI_16415 are separated by 340 nucleotides BNILDI_16400: pheT - phenylalanine--tRNA ligase subunit beta, at 3,283,871 to 3,286,258 pheT BNILDI_16405: BNILDI_16405 - Integration host factor subunit alpha, at 3,286,263 to 3,286,562 _16405 BNILDI_16410: BNILDI_16410 - hok/gef family protein, at 3,286,868 to 3,287,008 _16410 BNILDI_16415: BNILDI_16415 - Baseplate assembly protein J, at 3,287,349 to 3,287,657 _16415 Position (kb) 3286 3287Strain fitness (log2 ratio) -1 0 1at 3286.412 kb on + strand, within BNILDI_16405at 3286.481 kb on + strand, within BNILDI_16405at 3286.691 kb on + strandat 3286.711 kb on - strandat 3286.711 kb on - strandat 3286.712 kb on + strandat 3286.713 kb on - strandat 3286.801 kb on + strandat 3286.876 kb on + strandat 3286.877 kb on - strandat 3286.877 kb on - strandat 3286.877 kb on - strandat 3286.883 kb on + strand, within BNILDI_16410at 3286.884 kb on - strand, within BNILDI_16410at 3286.922 kb on + strand, within BNILDI_16410at 3287.009 kb on - strandat 3287.161 kb on - strandat 3287.212 kb on - strandat 3287.403 kb on + strand, within BNILDI_16415at 3287.440 kb on - strand, within BNILDI_16415

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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3,286,412 + BNILDI_16405 0.50 -1.4
3,286,481 + BNILDI_16405 0.73 -0.5
3,286,691 + +0.5
3,286,711 - +0.0
3,286,711 - +0.5
3,286,712 + +0.4
3,286,713 - -0.2
3,286,801 + -0.7
3,286,876 + -1.2
3,286,877 - +1.4
3,286,877 - -1.6
3,286,877 - -0.6
3,286,883 + BNILDI_16410 0.11 -0.6
3,286,884 - BNILDI_16410 0.11 -0.3
3,286,922 + BNILDI_16410 0.38 -0.1
3,287,009 - -0.9
3,287,161 - +1.2
3,287,212 - +0.0
3,287,403 + BNILDI_16415 0.17 +1.4
3,287,440 - BNILDI_16415 0.29 -0.3

Or see this region's nucleotide sequence