Strain Fitness in Escherichia coli ECRC62 around BNILDI_14660

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgalF and wzy are separated by 436 nucleotideswzy and BNILDI_14665 overlap by 4 nucleotidesBNILDI_14665 and wzx overlap by 28 nucleotides BNILDI_14655: galF - UTP--glucose-1-phosphate uridylyltransferase GalF, at 2,960,514 to 2,961,407 galF BNILDI_14660: wzy - O23 family O-antigen polymerase, at 2,961,844 to 2,962,869 wzy BNILDI_14665: BNILDI_14665 - GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase, at 2,962,866 to 2,963,735 _14665 BNILDI_14670: wzx - O23 family O-antigen flippase, at 2,963,708 to 2,964,913 wzx Position (kb) 2961 2962 2963Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2960.874 kb on - strand, within galFat 2961.011 kb on + strand, within galFat 2961.012 kb on - strand, within galFat 2961.012 kb on - strand, within galFat 2961.012 kb on - strand, within galFat 2961.187 kb on + strand, within galFat 2961.187 kb on + strand, within galFat 2961.187 kb on + strand, within galFat 2961.219 kb on - strand, within galFat 2961.320 kb on + strandat 2961.320 kb on + strandat 2961.321 kb on - strandat 2961.321 kb on - strandat 2961.321 kb on - strandat 2961.339 kb on + strandat 2961.437 kb on - strandat 2961.476 kb on - strandat 2961.683 kb on - strandat 2961.784 kb on + strandat 2962.724 kb on + strand, within wzyat 2962.729 kb on - strand, within wzy

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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2,960,874 - galF BNILDI_14655 0.40 +0.7
2,961,011 + galF BNILDI_14655 0.56 -0.1
2,961,012 - galF BNILDI_14655 0.56 -1.1
2,961,012 - galF BNILDI_14655 0.56 +0.7
2,961,012 - galF BNILDI_14655 0.56 +1.6
2,961,187 + galF BNILDI_14655 0.75 -0.9
2,961,187 + galF BNILDI_14655 0.75 +2.4
2,961,187 + galF BNILDI_14655 0.75 +0.8
2,961,219 - galF BNILDI_14655 0.79 +0.8
2,961,320 + -2.4
2,961,320 + -2.8
2,961,321 - -0.1
2,961,321 - -0.5
2,961,321 - +0.7
2,961,339 + -1.3
2,961,437 - -1.5
2,961,476 - -1.6
2,961,683 - -4.0
2,961,784 + -0.7
2,962,724 + wzy BNILDI_14660 0.86 -0.8
2,962,729 - wzy BNILDI_14660 0.86 -3.3

Or see this region's nucleotide sequence