Experiment: Bas05
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaD and wcaE are separated by 10 nucleotides wcaE and wcaF are separated by 15 nucleotides wcaF and BNILDI_14585 are separated by 25 nucleotides
BNILDI_14570: wcaD - putative colanic acid polymerase WcaD, at 2,943,691 to 2,944,908
wcaD
BNILDI_14575: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 2,944,919 to 2,945,665
wcaE
BNILDI_14580: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 2,945,681 to 2,946,229
wcaF
BNILDI_14585: BNILDI_14585 - GDP-mannose 4,6-dehydratase, at 2,946,255 to 2,947,376
_14585
Position (kb)
2944
2945
2946 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2943.931 kb on + strand, within wcaD at 2943.959 kb on - strand, within wcaD at 2943.961 kb on - strand, within wcaD at 2943.973 kb on - strand, within wcaD at 2943.991 kb on - strand, within wcaD at 2944.035 kb on - strand, within wcaD at 2944.965 kb on + strand at 2944.966 kb on - strand at 2945.011 kb on + strand, within wcaE at 2945.012 kb on - strand, within wcaE at 2945.653 kb on + strand at 2945.878 kb on + strand, within wcaF at 2945.993 kb on + strand, within wcaF at 2946.249 kb on + strand at 2946.249 kb on + strand at 2946.252 kb on + strand at 2946.253 kb on - strand at 2946.253 kb on - strand at 2946.253 kb on - strand at 2946.253 kb on - strand at 2946.347 kb on + strand at 2946.351 kb on - strand at 2946.439 kb on - strand, within BNILDI_14585 at 2946.439 kb on - strand, within BNILDI_14585 at 2946.439 kb on - strand, within BNILDI_14585 at 2946.455 kb on + strand, within BNILDI_14585 at 2946.456 kb on - strand, within BNILDI_14585 at 2946.456 kb on - strand, within BNILDI_14585 at 2946.456 kb on - strand, within BNILDI_14585
Per-strain Table
Position Strand Gene LocusTag Fraction Bas05 remove 2,943,931 + wcaD BNILDI_14570 0.20 -0.3 2,943,959 - wcaD BNILDI_14570 0.22 +2.7 2,943,961 - wcaD BNILDI_14570 0.22 -1.3 2,943,973 - wcaD BNILDI_14570 0.23 -0.6 2,943,991 - wcaD BNILDI_14570 0.25 +0.2 2,944,035 - wcaD BNILDI_14570 0.28 +1.4 2,944,965 + -1.8 2,944,966 - -0.3 2,945,011 + wcaE BNILDI_14575 0.12 -0.5 2,945,012 - wcaE BNILDI_14575 0.12 -0.6 2,945,653 + -2.0 2,945,878 + wcaF BNILDI_14580 0.36 +0.3 2,945,993 + wcaF BNILDI_14580 0.57 -0.7 2,946,249 + +0.0 2,946,249 + -0.0 2,946,252 + -0.5 2,946,253 - +0.5 2,946,253 - +0.4 2,946,253 - -0.1 2,946,253 - +1.5 2,946,347 + -0.3 2,946,351 - -0.2 2,946,439 - BNILDI_14585 0.16 +1.0 2,946,439 - BNILDI_14585 0.16 +0.1 2,946,439 - BNILDI_14585 0.16 +0.8 2,946,455 + BNILDI_14585 0.18 +1.3 2,946,456 - BNILDI_14585 0.18 -0.1 2,946,456 - BNILDI_14585 0.18 -0.3 2,946,456 - BNILDI_14585 0.18 +1.1
Or see this region's nucleotide sequence