Strain Fitness in Escherichia coli ECRC62 around BNILDI_14095

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgtdA and ycgM are separated by 15 nucleotidesycgM and maiA are separated by 8 nucleotidesmaiA and ubiH are separated by 14 nucleotides BNILDI_14085: gtdA - gentisate 1,2-dioxygenase, at 2,839,075 to 2,840,103 gtdA BNILDI_14090: ycgM - 5-carboxymethyl-2-hydroxymuconate isomerase, at 2,840,119 to 2,840,820 ycgM BNILDI_14095: maiA - maleylacetoacetate isomerase, at 2,840,829 to 2,841,473 maiA BNILDI_14100: ubiH - 3-hydroxybenzoate 6-monooxygenase, at 2,841,488 to 2,842,681 ubiH Position (kb) 2840 2841 2842Strain fitness (log2 ratio) -2 -1 0 1at 2839.912 kb on + strand, within gtdAat 2839.912 kb on + strand, within gtdAat 2839.913 kb on - strand, within gtdAat 2839.981 kb on - strand, within gtdAat 2840.031 kb on + strandat 2840.128 kb on + strandat 2840.129 kb on - strandat 2840.132 kb on + strandat 2840.133 kb on - strandat 2840.204 kb on - strand, within ycgMat 2840.217 kb on + strand, within ycgMat 2840.218 kb on - strand, within ycgMat 2840.360 kb on - strand, within ycgMat 2840.410 kb on - strand, within ycgMat 2840.434 kb on + strand, within ycgMat 2840.434 kb on + strand, within ycgMat 2840.435 kb on - strand, within ycgMat 2840.583 kb on + strand, within ycgMat 2840.680 kb on + strand, within ycgMat 2840.839 kb on - strandat 2841.058 kb on - strand, within maiAat 2841.207 kb on - strand, within maiAat 2841.400 kb on - strand, within maiAat 2841.941 kb on - strand, within ubiHat 2842.002 kb on + strand, within ubiHat 2842.081 kb on - strand, within ubiHat 2842.157 kb on + strand, within ubiHat 2842.157 kb on + strand, within ubiHat 2842.158 kb on - strand, within ubiHat 2842.159 kb on + strand, within ubiHat 2842.389 kb on - strand, within ubiHat 2842.389 kb on - strand, within ubiHat 2842.391 kb on - strand, within ubiH

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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2,839,912 + gtdA BNILDI_14085 0.81 -1.0
2,839,912 + gtdA BNILDI_14085 0.81 -0.2
2,839,913 - gtdA BNILDI_14085 0.81 +0.1
2,839,981 - gtdA BNILDI_14085 0.88 +0.7
2,840,031 + +1.1
2,840,128 + +1.0
2,840,129 - -1.0
2,840,132 + +0.1
2,840,133 - +0.7
2,840,204 - ycgM BNILDI_14090 0.12 +1.1
2,840,217 + ycgM BNILDI_14090 0.14 -0.1
2,840,218 - ycgM BNILDI_14090 0.14 -0.1
2,840,360 - ycgM BNILDI_14090 0.34 -1.0
2,840,410 - ycgM BNILDI_14090 0.41 +0.1
2,840,434 + ycgM BNILDI_14090 0.45 -0.1
2,840,434 + ycgM BNILDI_14090 0.45 +1.0
2,840,435 - ycgM BNILDI_14090 0.45 +0.0
2,840,583 + ycgM BNILDI_14090 0.66 +0.6
2,840,680 + ycgM BNILDI_14090 0.80 -0.9
2,840,839 - +0.4
2,841,058 - maiA BNILDI_14095 0.36 +1.5
2,841,207 - maiA BNILDI_14095 0.59 -2.6
2,841,400 - maiA BNILDI_14095 0.89 -0.9
2,841,941 - ubiH BNILDI_14100 0.38 +0.8
2,842,002 + ubiH BNILDI_14100 0.43 -0.6
2,842,081 - ubiH BNILDI_14100 0.50 -0.4
2,842,157 + ubiH BNILDI_14100 0.56 +0.2
2,842,157 + ubiH BNILDI_14100 0.56 -0.4
2,842,158 - ubiH BNILDI_14100 0.56 +1.2
2,842,159 + ubiH BNILDI_14100 0.56 -2.0
2,842,389 - ubiH BNILDI_14100 0.75 -0.2
2,842,389 - ubiH BNILDI_14100 0.75 -0.7
2,842,391 - ubiH BNILDI_14100 0.76 -0.2

Or see this region's nucleotide sequence