Experiment: Bas05
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoA and nuoB are separated by 15 nucleotides nuoB and nuoC are separated by 93 nucleotides nuoC and nuoE are separated by 2 nucleotides nuoE and nuoF overlap by 4 nucleotides
BNILDI_13380: nuoA - NADH-quinone oxidoreductase subunit NuoA, at 2,679,993 to 2,680,436
nuoA
BNILDI_13385: nuoB - NADH-quinone oxidoreductase subunit NuoB, at 2,680,452 to 2,681,114
nuoB
BNILDI_13390: nuoC - NADH-quinone oxidoreductase subunit C/D, at 2,681,208 to 2,683,010
nuoC
BNILDI_13395: nuoE - NADH-quinone oxidoreductase subunit NuoE, at 2,683,013 to 2,683,513
nuoE
BNILDI_13400: nuoF - NADH-quinone oxidoreductase subunit NuoF, at 2,683,510 to 2,684,847
nuoF
Position (kb)
2681
2682
2683
2684 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2680.242 kb on + strand, within nuoA at 2680.242 kb on + strand, within nuoA at 2680.243 kb on - strand, within nuoA at 2680.395 kb on - strand at 2680.449 kb on - strand at 2680.645 kb on - strand, within nuoB at 2680.858 kb on - strand, within nuoB at 2680.858 kb on - strand, within nuoB at 2680.911 kb on + strand, within nuoB at 2680.911 kb on + strand, within nuoB at 2680.912 kb on - strand, within nuoB at 2680.944 kb on + strand, within nuoB at 2680.944 kb on + strand, within nuoB at 2681.096 kb on + strand at 2681.150 kb on - strand at 2681.219 kb on + strand at 2681.220 kb on - strand at 2681.419 kb on - strand, within nuoC at 2681.419 kb on - strand, within nuoC at 2681.419 kb on - strand, within nuoC at 2681.616 kb on + strand, within nuoC at 2681.616 kb on + strand, within nuoC at 2681.835 kb on - strand, within nuoC at 2682.067 kb on - strand, within nuoC at 2682.153 kb on - strand, within nuoC at 2682.171 kb on + strand, within nuoC at 2682.171 kb on + strand, within nuoC at 2682.173 kb on + strand, within nuoC at 2682.185 kb on + strand, within nuoC at 2682.185 kb on + strand, within nuoC at 2682.185 kb on + strand, within nuoC at 2682.538 kb on - strand, within nuoC at 2682.597 kb on + strand, within nuoC at 2682.844 kb on - strand at 2683.306 kb on + strand, within nuoE at 2683.350 kb on + strand, within nuoE at 2683.508 kb on - strand at 2683.595 kb on - strand at 2683.627 kb on - strand at 2683.671 kb on - strand, within nuoF at 2683.805 kb on - strand, within nuoF at 2683.916 kb on - strand, within nuoF
Per-strain Table
Position Strand Gene LocusTag Fraction Bas05 remove 2,680,242 + nuoA BNILDI_13380 0.56 +1.0 2,680,242 + nuoA BNILDI_13380 0.56 -0.0 2,680,243 - nuoA BNILDI_13380 0.56 -0.8 2,680,395 - -1.0 2,680,449 - -0.3 2,680,645 - nuoB BNILDI_13385 0.29 -1.6 2,680,858 - nuoB BNILDI_13385 0.61 +3.6 2,680,858 - nuoB BNILDI_13385 0.61 -0.0 2,680,911 + nuoB BNILDI_13385 0.69 -0.6 2,680,911 + nuoB BNILDI_13385 0.69 -0.3 2,680,912 - nuoB BNILDI_13385 0.69 +0.1 2,680,944 + nuoB BNILDI_13385 0.74 +1.6 2,680,944 + nuoB BNILDI_13385 0.74 +0.1 2,681,096 + +1.1 2,681,150 - -0.0 2,681,219 + -0.5 2,681,220 - +2.5 2,681,419 - nuoC BNILDI_13390 0.12 -1.0 2,681,419 - nuoC BNILDI_13390 0.12 -0.6 2,681,419 - nuoC BNILDI_13390 0.12 -2.0 2,681,616 + nuoC BNILDI_13390 0.23 -0.9 2,681,616 + nuoC BNILDI_13390 0.23 -0.0 2,681,835 - nuoC BNILDI_13390 0.35 -1.4 2,682,067 - nuoC BNILDI_13390 0.48 -1.0 2,682,153 - nuoC BNILDI_13390 0.52 -2.3 2,682,171 + nuoC BNILDI_13390 0.53 -1.5 2,682,171 + nuoC BNILDI_13390 0.53 +1.7 2,682,173 + nuoC BNILDI_13390 0.54 -1.7 2,682,185 + nuoC BNILDI_13390 0.54 -1.3 2,682,185 + nuoC BNILDI_13390 0.54 +0.7 2,682,185 + nuoC BNILDI_13390 0.54 +0.6 2,682,538 - nuoC BNILDI_13390 0.74 -1.6 2,682,597 + nuoC BNILDI_13390 0.77 -1.0 2,682,844 - +0.7 2,683,306 + nuoE BNILDI_13395 0.58 +1.3 2,683,350 + nuoE BNILDI_13395 0.67 -3.1 2,683,508 - -0.7 2,683,595 - +0.3 2,683,627 - -1.7 2,683,671 - nuoF BNILDI_13400 0.12 +0.7 2,683,805 - nuoF BNILDI_13400 0.22 -1.0 2,683,916 - nuoF BNILDI_13400 0.30 -0.4
Or see this region's nucleotide sequence