Experiment: Bas05
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt zapA and ygfB are separated by 167 nucleotides ygfB and pepP are separated by 25 nucleotides pepP and ubiH overlap by 4 nucleotides
BNILDI_09940: zapA - cell division protein ZapA, at 1,995,906 to 1,996,235
zapA
BNILDI_09945: ygfB - UPF0149 family protein YgfB, at 1,996,403 to 1,996,981
ygfB
BNILDI_09950: pepP - Xaa-Pro aminopeptidase, at 1,997,007 to 1,998,332
pepP
BNILDI_09955: ubiH - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 1,998,329 to 1,999,507
ubiH
Position (kb)
1997
1998
1999 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1996.048 kb on + strand, within zapA at 1996.088 kb on + strand, within zapA at 1996.101 kb on - strand, within zapA at 1996.303 kb on + strand at 1996.304 kb on - strand at 1996.304 kb on - strand at 1996.304 kb on - strand at 1996.304 kb on - strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.306 kb on - strand at 1996.306 kb on - strand at 1996.306 kb on - strand at 1996.306 kb on - strand at 1996.306 kb on - strand at 1996.310 kb on - strand at 1996.363 kb on - strand at 1996.367 kb on + strand at 1996.367 kb on + strand at 1996.367 kb on + strand at 1996.368 kb on - strand at 1996.368 kb on - strand at 1996.368 kb on - strand at 1996.402 kb on + strand at 1996.403 kb on - strand at 1996.410 kb on + strand at 1996.512 kb on + strand, within ygfB at 1996.512 kb on + strand, within ygfB at 1996.513 kb on - strand, within ygfB at 1996.549 kb on - strand, within ygfB at 1996.609 kb on + strand, within ygfB at 1996.667 kb on + strand, within ygfB at 1996.802 kb on - strand, within ygfB at 1996.802 kb on - strand, within ygfB at 1996.892 kb on + strand, within ygfB at 1996.892 kb on + strand, within ygfB at 1996.893 kb on - strand, within ygfB at 1996.974 kb on + strand at 1996.974 kb on + strand at 1996.975 kb on - strand at 1996.975 kb on - strand at 1997.107 kb on + strand at 1997.391 kb on + strand, within pepP at 1997.694 kb on + strand, within pepP at 1997.696 kb on + strand, within pepP at 1997.877 kb on + strand, within pepP at 1997.877 kb on + strand, within pepP at 1998.053 kb on + strand, within pepP at 1998.082 kb on + strand, within pepP at 1998.105 kb on + strand, within pepP at 1998.105 kb on + strand, within pepP at 1998.145 kb on + strand, within pepP at 1998.168 kb on + strand, within pepP
Per-strain Table
Position Strand Gene LocusTag Fraction Bas05 remove 1,996,048 + zapA BNILDI_09940 0.43 +0.0 1,996,088 + zapA BNILDI_09940 0.55 +0.4 1,996,101 - zapA BNILDI_09940 0.59 +0.6 1,996,303 + +1.0 1,996,304 - -0.2 1,996,304 - -0.8 1,996,304 - -0.3 1,996,304 - +1.0 1,996,305 + +0.4 1,996,305 + -0.1 1,996,305 + -1.8 1,996,305 + -2.2 1,996,305 + -0.6 1,996,305 + -2.9 1,996,305 + +0.7 1,996,305 + -0.3 1,996,305 + +0.7 1,996,306 - +0.0 1,996,306 - -0.6 1,996,306 - -0.7 1,996,306 - -0.1 1,996,306 - +0.4 1,996,310 - -0.6 1,996,363 - -0.7 1,996,367 + -1.0 1,996,367 + +0.8 1,996,367 + +0.7 1,996,368 - -0.2 1,996,368 - -0.8 1,996,368 - -0.4 1,996,402 + -0.3 1,996,403 - -0.2 1,996,410 + +0.1 1,996,512 + ygfB BNILDI_09945 0.19 +0.3 1,996,512 + ygfB BNILDI_09945 0.19 +0.0 1,996,513 - ygfB BNILDI_09945 0.19 -0.8 1,996,549 - ygfB BNILDI_09945 0.25 -1.1 1,996,609 + ygfB BNILDI_09945 0.36 -0.1 1,996,667 + ygfB BNILDI_09945 0.46 +0.9 1,996,802 - ygfB BNILDI_09945 0.69 +0.2 1,996,802 - ygfB BNILDI_09945 0.69 -0.7 1,996,892 + ygfB BNILDI_09945 0.84 +1.3 1,996,892 + ygfB BNILDI_09945 0.84 -0.2 1,996,893 - ygfB BNILDI_09945 0.85 -1.8 1,996,974 + -0.3 1,996,974 + +0.7 1,996,975 - -0.9 1,996,975 - -0.3 1,997,107 + -2.0 1,997,391 + pepP BNILDI_09950 0.29 -1.6 1,997,694 + pepP BNILDI_09950 0.52 -0.5 1,997,696 + pepP BNILDI_09950 0.52 +0.7 1,997,877 + pepP BNILDI_09950 0.66 -2.2 1,997,877 + pepP BNILDI_09950 0.66 +0.4 1,998,053 + pepP BNILDI_09950 0.79 -1.0 1,998,082 + pepP BNILDI_09950 0.81 -2.8 1,998,105 + pepP BNILDI_09950 0.83 -1.0 1,998,105 + pepP BNILDI_09950 0.83 -1.1 1,998,145 + pepP BNILDI_09950 0.86 -0.7 1,998,168 + pepP BNILDI_09950 0.88 -0.7
Or see this region's nucleotide sequence