Strain Fitness in Escherichia coli ECRC62 around BNILDI_09950

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntzapA and ygfB are separated by 167 nucleotidesygfB and pepP are separated by 25 nucleotidespepP and ubiH overlap by 4 nucleotides BNILDI_09940: zapA - cell division protein ZapA, at 1,995,906 to 1,996,235 zapA BNILDI_09945: ygfB - UPF0149 family protein YgfB, at 1,996,403 to 1,996,981 ygfB BNILDI_09950: pepP - Xaa-Pro aminopeptidase, at 1,997,007 to 1,998,332 pepP BNILDI_09955: ubiH - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 1,998,329 to 1,999,507 ubiH Position (kb) 1997 1998 1999Strain fitness (log2 ratio) -3 -2 -1 0 1at 1996.048 kb on + strand, within zapAat 1996.088 kb on + strand, within zapAat 1996.101 kb on - strand, within zapAat 1996.303 kb on + strandat 1996.304 kb on - strandat 1996.304 kb on - strandat 1996.304 kb on - strandat 1996.304 kb on - strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.306 kb on - strandat 1996.306 kb on - strandat 1996.306 kb on - strandat 1996.306 kb on - strandat 1996.306 kb on - strandat 1996.310 kb on - strandat 1996.363 kb on - strandat 1996.367 kb on + strandat 1996.367 kb on + strandat 1996.367 kb on + strandat 1996.368 kb on - strandat 1996.368 kb on - strandat 1996.368 kb on - strandat 1996.402 kb on + strandat 1996.403 kb on - strandat 1996.410 kb on + strandat 1996.512 kb on + strand, within ygfBat 1996.512 kb on + strand, within ygfBat 1996.513 kb on - strand, within ygfBat 1996.549 kb on - strand, within ygfBat 1996.609 kb on + strand, within ygfBat 1996.667 kb on + strand, within ygfBat 1996.802 kb on - strand, within ygfBat 1996.802 kb on - strand, within ygfBat 1996.892 kb on + strand, within ygfBat 1996.892 kb on + strand, within ygfBat 1996.893 kb on - strand, within ygfBat 1996.974 kb on + strandat 1996.974 kb on + strandat 1996.975 kb on - strandat 1996.975 kb on - strandat 1997.107 kb on + strandat 1997.391 kb on + strand, within pepPat 1997.694 kb on + strand, within pepPat 1997.696 kb on + strand, within pepPat 1997.877 kb on + strand, within pepPat 1997.877 kb on + strand, within pepPat 1998.053 kb on + strand, within pepPat 1998.082 kb on + strand, within pepPat 1998.105 kb on + strand, within pepPat 1998.105 kb on + strand, within pepPat 1998.145 kb on + strand, within pepPat 1998.168 kb on + strand, within pepP

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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1,996,048 + zapA BNILDI_09940 0.43 +0.0
1,996,088 + zapA BNILDI_09940 0.55 +0.4
1,996,101 - zapA BNILDI_09940 0.59 +0.6
1,996,303 + +1.0
1,996,304 - -0.2
1,996,304 - -0.8
1,996,304 - -0.3
1,996,304 - +1.0
1,996,305 + +0.4
1,996,305 + -0.1
1,996,305 + -1.8
1,996,305 + -2.2
1,996,305 + -0.6
1,996,305 + -2.9
1,996,305 + +0.7
1,996,305 + -0.3
1,996,305 + +0.7
1,996,306 - +0.0
1,996,306 - -0.6
1,996,306 - -0.7
1,996,306 - -0.1
1,996,306 - +0.4
1,996,310 - -0.6
1,996,363 - -0.7
1,996,367 + -1.0
1,996,367 + +0.8
1,996,367 + +0.7
1,996,368 - -0.2
1,996,368 - -0.8
1,996,368 - -0.4
1,996,402 + -0.3
1,996,403 - -0.2
1,996,410 + +0.1
1,996,512 + ygfB BNILDI_09945 0.19 +0.3
1,996,512 + ygfB BNILDI_09945 0.19 +0.0
1,996,513 - ygfB BNILDI_09945 0.19 -0.8
1,996,549 - ygfB BNILDI_09945 0.25 -1.1
1,996,609 + ygfB BNILDI_09945 0.36 -0.1
1,996,667 + ygfB BNILDI_09945 0.46 +0.9
1,996,802 - ygfB BNILDI_09945 0.69 +0.2
1,996,802 - ygfB BNILDI_09945 0.69 -0.7
1,996,892 + ygfB BNILDI_09945 0.84 +1.3
1,996,892 + ygfB BNILDI_09945 0.84 -0.2
1,996,893 - ygfB BNILDI_09945 0.85 -1.8
1,996,974 + -0.3
1,996,974 + +0.7
1,996,975 - -0.9
1,996,975 - -0.3
1,997,107 + -2.0
1,997,391 + pepP BNILDI_09950 0.29 -1.6
1,997,694 + pepP BNILDI_09950 0.52 -0.5
1,997,696 + pepP BNILDI_09950 0.52 +0.7
1,997,877 + pepP BNILDI_09950 0.66 -2.2
1,997,877 + pepP BNILDI_09950 0.66 +0.4
1,998,053 + pepP BNILDI_09950 0.79 -1.0
1,998,082 + pepP BNILDI_09950 0.81 -2.8
1,998,105 + pepP BNILDI_09950 0.83 -1.0
1,998,105 + pepP BNILDI_09950 0.83 -1.1
1,998,145 + pepP BNILDI_09950 0.86 -0.7
1,998,168 + pepP BNILDI_09950 0.88 -0.7

Or see this region's nucleotide sequence