Strain Fitness in Escherichia coli ECRC62 around BNILDI_09775

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntloiP and tkt are separated by 277 nucleotidestkt and cmtB are separated by 313 nucleotidescmtB and cmtA are separated by 27 nucleotides BNILDI_09770: loiP - metalloprotease LoiP, at 1,965,817 to 1,966,575 loiP BNILDI_09775: tkt - transketolase, at 1,966,853 to 1,968,844 tkt BNILDI_09780: cmtB - PTS mannitol transporter subunit IIA, at 1,969,158 to 1,969,601 cmtB BNILDI_09785: cmtA - PTS mannitol transporter subunit IICB, at 1,969,629 to 1,971,017 cmtA Position (kb) 1966 1967 1968 1969Strain fitness (log2 ratio) -1 0 1 2at 1966.111 kb on + strand, within loiPat 1966.129 kb on - strand, within loiPat 1966.224 kb on + strand, within loiPat 1966.224 kb on + strand, within loiPat 1966.224 kb on + strand, within loiPat 1966.295 kb on + strand, within loiPat 1966.295 kb on + strand, within loiPat 1966.295 kb on + strand, within loiPat 1966.295 kb on + strand, within loiPat 1966.295 kb on + strand, within loiPat 1966.296 kb on - strand, within loiPat 1966.296 kb on - strand, within loiPat 1966.296 kb on - strand, within loiPat 1966.326 kb on + strand, within loiPat 1966.350 kb on + strand, within loiPat 1966.350 kb on + strand, within loiPat 1966.351 kb on - strand, within loiPat 1966.351 kb on - strand, within loiPat 1966.351 kb on - strand, within loiPat 1966.351 kb on - strand, within loiPat 1966.370 kb on + strand, within loiPat 1966.513 kb on - strandat 1966.559 kb on - strandat 1966.653 kb on - strandat 1966.680 kb on - strandat 1966.741 kb on + strandat 1966.864 kb on + strandat 1966.882 kb on + strandat 1967.067 kb on - strand, within tktat 1967.137 kb on + strand, within tktat 1967.294 kb on + strand, within tktat 1967.301 kb on - strand, within tktat 1967.693 kb on + strand, within tktat 1968.062 kb on + strand, within tktat 1968.320 kb on - strand, within tktat 1968.684 kb on + strandat 1968.705 kb on - strandat 1968.717 kb on - strandat 1968.842 kb on + strandat 1968.847 kb on - strandat 1968.975 kb on - strandat 1969.310 kb on + strand, within cmtBat 1969.327 kb on - strand, within cmtBat 1969.511 kb on - strand, within cmtBat 1969.743 kb on - strandat 1969.800 kb on - strand, within cmtAat 1969.830 kb on - strand, within cmtAat 1969.830 kb on - strand, within cmtA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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1,966,111 + loiP BNILDI_09770 0.39 -0.6
1,966,129 - loiP BNILDI_09770 0.41 -0.2
1,966,224 + loiP BNILDI_09770 0.54 +0.4
1,966,224 + loiP BNILDI_09770 0.54 -0.2
1,966,224 + loiP BNILDI_09770 0.54 -0.1
1,966,295 + loiP BNILDI_09770 0.63 -0.3
1,966,295 + loiP BNILDI_09770 0.63 -0.4
1,966,295 + loiP BNILDI_09770 0.63 -0.6
1,966,295 + loiP BNILDI_09770 0.63 +0.6
1,966,295 + loiP BNILDI_09770 0.63 -0.7
1,966,296 - loiP BNILDI_09770 0.63 -1.0
1,966,296 - loiP BNILDI_09770 0.63 +0.7
1,966,296 - loiP BNILDI_09770 0.63 +1.0
1,966,326 + loiP BNILDI_09770 0.67 -0.0
1,966,350 + loiP BNILDI_09770 0.70 +1.2
1,966,350 + loiP BNILDI_09770 0.70 -0.8
1,966,351 - loiP BNILDI_09770 0.70 +0.1
1,966,351 - loiP BNILDI_09770 0.70 +0.7
1,966,351 - loiP BNILDI_09770 0.70 -0.2
1,966,351 - loiP BNILDI_09770 0.70 +1.9
1,966,370 + loiP BNILDI_09770 0.73 -0.4
1,966,513 - -0.4
1,966,559 - -1.4
1,966,653 - +0.4
1,966,680 - +0.1
1,966,741 + -0.6
1,966,864 + +0.4
1,966,882 + -1.1
1,967,067 - tkt BNILDI_09775 0.11 -1.0
1,967,137 + tkt BNILDI_09775 0.14 +0.3
1,967,294 + tkt BNILDI_09775 0.22 -0.8
1,967,301 - tkt BNILDI_09775 0.22 +1.4
1,967,693 + tkt BNILDI_09775 0.42 -1.3
1,968,062 + tkt BNILDI_09775 0.61 -1.0
1,968,320 - tkt BNILDI_09775 0.74 -0.2
1,968,684 + -0.4
1,968,705 - -1.5
1,968,717 - +0.0
1,968,842 + -0.3
1,968,847 - +1.0
1,968,975 - +0.2
1,969,310 + cmtB BNILDI_09780 0.34 +0.1
1,969,327 - cmtB BNILDI_09780 0.38 -0.6
1,969,511 - cmtB BNILDI_09780 0.80 +1.0
1,969,743 - -1.3
1,969,800 - cmtA BNILDI_09785 0.12 -0.8
1,969,830 - cmtA BNILDI_09785 0.14 -0.4
1,969,830 - cmtA BNILDI_09785 0.14 +0.1

Or see this region's nucleotide sequence