Strain Fitness in Escherichia coli ECRC62 around BNILDI_09725

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntruvX and yqgE overlap by 1 nucleotidesyqgE and gshB are separated by 36 nucleotidesgshB and rsmE are separated by 12 nucleotidesrsmE and endA are separated by 79 nucleotides BNILDI_09715: ruvX - Holliday junction resolvase RuvX, at 1,953,685 to 1,954,101 ruvX BNILDI_09720: yqgE - YqgE/AlgH family protein, at 1,954,101 to 1,954,736 yqgE BNILDI_09725: gshB - glutathione synthase, at 1,954,773 to 1,955,723 gshB BNILDI_09730: rsmE - 16S rRNA (uracil(1498)-N(3))-methyltransferase, at 1,955,736 to 1,956,467 rsmE BNILDI_09735: endA - deoxyribonuclease I, at 1,956,547 to 1,957,254 endA Position (kb) 1954 1955 1956Strain fitness (log2 ratio) -2 -1 0 1 2at 1954.294 kb on - strand, within yqgEat 1954.294 kb on - strand, within yqgEat 1954.578 kb on - strand, within yqgEat 1954.578 kb on - strand, within yqgEat 1954.591 kb on + strand, within yqgEat 1954.592 kb on - strand, within yqgEat 1954.809 kb on + strandat 1954.877 kb on + strand, within gshBat 1954.878 kb on - strand, within gshBat 1955.323 kb on - strand, within gshBat 1955.323 kb on - strand, within gshBat 1955.424 kb on - strand, within gshBat 1955.435 kb on - strand, within gshBat 1955.435 kb on - strand, within gshBat 1955.466 kb on - strand, within gshBat 1955.612 kb on + strand, within gshBat 1955.613 kb on - strand, within gshBat 1955.614 kb on + strand, within gshBat 1955.615 kb on - strand, within gshBat 1955.757 kb on + strandat 1955.764 kb on - strandat 1955.927 kb on + strand, within rsmEat 1956.230 kb on + strand, within rsmEat 1956.375 kb on + strand, within rsmEat 1956.448 kb on + strandat 1956.449 kb on - strandat 1956.467 kb on + strandat 1956.467 kb on + strandat 1956.467 kb on + strandat 1956.503 kb on - strandat 1956.543 kb on + strandat 1956.543 kb on + strandat 1956.544 kb on - strandat 1956.581 kb on + strandat 1956.581 kb on + strandat 1956.582 kb on - strandat 1956.651 kb on - strand, within endAat 1956.707 kb on + strand, within endAat 1956.722 kb on + strand, within endAat 1956.722 kb on + strand, within endAat 1956.722 kb on + strand, within endAat 1956.722 kb on + strand, within endAat 1956.722 kb on + strand, within endAat 1956.723 kb on - strand, within endAat 1956.723 kb on - strand, within endAat 1956.723 kb on - strand, within endAat 1956.723 kb on - strand, within endA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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1,954,294 - yqgE BNILDI_09720 0.30 +0.0
1,954,294 - yqgE BNILDI_09720 0.30 -1.3
1,954,578 - yqgE BNILDI_09720 0.75 +0.2
1,954,578 - yqgE BNILDI_09720 0.75 -0.1
1,954,591 + yqgE BNILDI_09720 0.77 -0.4
1,954,592 - yqgE BNILDI_09720 0.77 -0.0
1,954,809 + -0.5
1,954,877 + gshB BNILDI_09725 0.11 -0.7
1,954,878 - gshB BNILDI_09725 0.11 -1.9
1,955,323 - gshB BNILDI_09725 0.58 +0.3
1,955,323 - gshB BNILDI_09725 0.58 -1.1
1,955,424 - gshB BNILDI_09725 0.68 -1.9
1,955,435 - gshB BNILDI_09725 0.70 -1.0
1,955,435 - gshB BNILDI_09725 0.70 -2.1
1,955,466 - gshB BNILDI_09725 0.73 -1.2
1,955,612 + gshB BNILDI_09725 0.88 +0.3
1,955,613 - gshB BNILDI_09725 0.88 +1.4
1,955,614 + gshB BNILDI_09725 0.88 +0.6
1,955,615 - gshB BNILDI_09725 0.89 -1.1
1,955,757 + +0.0
1,955,764 - +0.8
1,955,927 + rsmE BNILDI_09730 0.26 +1.0
1,956,230 + rsmE BNILDI_09730 0.67 -1.3
1,956,375 + rsmE BNILDI_09730 0.87 +0.6
1,956,448 + +0.2
1,956,449 - +0.8
1,956,467 + -1.1
1,956,467 + -0.2
1,956,467 + +0.4
1,956,503 - +0.0
1,956,543 + +1.8
1,956,543 + -0.2
1,956,544 - -0.0
1,956,581 + -0.2
1,956,581 + -0.0
1,956,582 - +0.5
1,956,651 - endA BNILDI_09735 0.15 -0.3
1,956,707 + endA BNILDI_09735 0.23 +0.9
1,956,722 + endA BNILDI_09735 0.25 -0.4
1,956,722 + endA BNILDI_09735 0.25 -0.1
1,956,722 + endA BNILDI_09735 0.25 -1.8
1,956,722 + endA BNILDI_09735 0.25 -0.3
1,956,722 + endA BNILDI_09735 0.25 -0.6
1,956,723 - endA BNILDI_09735 0.25 +0.1
1,956,723 - endA BNILDI_09735 0.25 -0.1
1,956,723 - endA BNILDI_09735 0.25 +0.8
1,956,723 - endA BNILDI_09735 0.25 +0.7

Or see this region's nucleotide sequence