Strain Fitness in Escherichia coli ECRC62 around BNILDI_09050

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntclcA and yadW are separated by 23 nucleotidesyadW and erpA overlap by 8 nucleotideserpA and yadS are separated by 46 nucleotidesyadS and btuF are separated by 37 nucleotidesbtuF and mtnN overlap by 8 nucleotides BNILDI_09035: clcA - H(+)/Cl(-) exchange transporter ClcA, at 1,813,457 to 1,814,878 clcA BNILDI_09040: yadW - Protein YadW, at 1,814,902 to 1,814,967 yadW BNILDI_09045: erpA - iron-sulfur cluster insertion protein ErpA, at 1,814,960 to 1,815,304 erpA BNILDI_09050: yadS - UPF0126 inner membrane protein YadS, at 1,815,351 to 1,815,974 yadS BNILDI_09055: btuF - vitamin B12 ABC transporter substrate-binding protein BtuF, at 1,816,012 to 1,816,812 btuF BNILDI_09060: mtnN - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 1,816,805 to 1,817,503 mtnN Position (kb) 1815 1816Strain fitness (log2 ratio) -2 -1 0 1 2at 1814.462 kb on - strand, within clcAat 1814.490 kb on + strand, within clcAat 1814.491 kb on - strand, within clcAat 1814.601 kb on - strand, within clcAat 1814.660 kb on - strand, within clcAat 1814.711 kb on + strand, within clcAat 1814.712 kb on - strand, within clcAat 1814.712 kb on - strand, within clcAat 1814.734 kb on + strand, within clcAat 1814.735 kb on - strand, within clcAat 1814.757 kb on + strandat 1814.758 kb on - strandat 1814.789 kb on + strandat 1814.789 kb on + strandat 1814.901 kb on - strandat 1814.910 kb on + strand, within yadWat 1815.302 kb on + strandat 1815.303 kb on - strandat 1815.330 kb on + strandat 1815.331 kb on - strandat 1815.389 kb on + strandat 1815.389 kb on + strandat 1815.390 kb on - strandat 1815.478 kb on + strand, within yadSat 1815.612 kb on - strand, within yadSat 1815.612 kb on - strand, within yadSat 1815.937 kb on - strandat 1815.951 kb on + strandat 1815.951 kb on + strandat 1815.952 kb on - strandat 1815.952 kb on - strandat 1815.964 kb on + strandat 1815.965 kb on - strandat 1816.014 kb on - strandat 1816.018 kb on + strandat 1816.062 kb on + strandat 1816.063 kb on - strandat 1816.134 kb on - strand, within btuFat 1816.198 kb on + strand, within btuFat 1816.199 kb on - strand, within btuFat 1816.549 kb on - strand, within btuFat 1816.664 kb on + strand, within btuFat 1816.665 kb on - strand, within btuFat 1816.665 kb on - strand, within btuFat 1816.806 kb on + strandat 1816.807 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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1,814,462 - clcA BNILDI_09035 0.71 +0.8
1,814,490 + clcA BNILDI_09035 0.73 -0.4
1,814,491 - clcA BNILDI_09035 0.73 +0.2
1,814,601 - clcA BNILDI_09035 0.80 -0.6
1,814,660 - clcA BNILDI_09035 0.85 +2.0
1,814,711 + clcA BNILDI_09035 0.88 +1.0
1,814,712 - clcA BNILDI_09035 0.88 -0.6
1,814,712 - clcA BNILDI_09035 0.88 +0.0
1,814,734 + clcA BNILDI_09035 0.90 -0.5
1,814,735 - clcA BNILDI_09035 0.90 -2.3
1,814,757 + +0.2
1,814,758 - -0.1
1,814,789 + +0.1
1,814,789 + +0.8
1,814,901 - +1.4
1,814,910 + yadW BNILDI_09040 0.12 -1.1
1,815,302 + +0.1
1,815,303 - +1.2
1,815,330 + +0.7
1,815,331 - -0.6
1,815,389 + +0.4
1,815,389 + -2.6
1,815,390 - +0.2
1,815,478 + yadS BNILDI_09050 0.20 -0.9
1,815,612 - yadS BNILDI_09050 0.42 +0.7
1,815,612 - yadS BNILDI_09050 0.42 -1.4
1,815,937 - -2.1
1,815,951 + -0.0
1,815,951 + -0.1
1,815,952 - -1.2
1,815,952 - -1.6
1,815,964 + -2.1
1,815,965 - +1.1
1,816,014 - +0.0
1,816,018 + +0.1
1,816,062 + -0.2
1,816,063 - +0.2
1,816,134 - btuF BNILDI_09055 0.15 -2.1
1,816,198 + btuF BNILDI_09055 0.23 +0.7
1,816,199 - btuF BNILDI_09055 0.23 -0.1
1,816,549 - btuF BNILDI_09055 0.67 -0.7
1,816,664 + btuF BNILDI_09055 0.81 -0.2
1,816,665 - btuF BNILDI_09055 0.82 +0.2
1,816,665 - btuF BNILDI_09055 0.82 +0.4
1,816,806 + -0.6
1,816,807 - -0.2

Or see this region's nucleotide sequence