Strain Fitness in Escherichia coli ECRC62 around BNILDI_08975

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpcnB and gluQRS are separated by 92 nucleotidesgluQRS and dksA are separated by 36 nucleotidesdksA and sfsA are separated by 177 nucleotides BNILDI_08970: pcnB - polynucleotide adenylyltransferase PcnB, at 1,796,202 to 1,797,566 pcnB BNILDI_08975: gluQRS - tRNA glutamyl-Q(34) synthetase GluQRS, at 1,797,659 to 1,798,585 gluQRS BNILDI_08980: dksA - RNA polymerase-binding protein DksA, at 1,798,622 to 1,799,077 dksA BNILDI_08985: sfsA - DNA/RNA nuclease SfsA, at 1,799,255 to 1,799,959 sfsA Position (kb) 1797 1798 1799Strain fitness (log2 ratio) -2 -1 0 1 2at 1796.812 kb on + strand, within pcnBat 1796.846 kb on + strand, within pcnBat 1796.979 kb on + strand, within pcnBat 1797.062 kb on - strand, within pcnBat 1797.092 kb on - strand, within pcnBat 1797.108 kb on - strand, within pcnBat 1797.443 kb on - strandat 1797.448 kb on - strandat 1797.600 kb on - strandat 1797.603 kb on - strandat 1797.655 kb on + strandat 1797.912 kb on + strand, within gluQRSat 1798.073 kb on - strand, within gluQRSat 1798.108 kb on + strand, within gluQRSat 1798.246 kb on + strand, within gluQRSat 1798.258 kb on - strand, within gluQRSat 1798.261 kb on - strand, within gluQRSat 1798.265 kb on + strand, within gluQRSat 1798.265 kb on + strand, within gluQRSat 1798.266 kb on - strand, within gluQRSat 1798.266 kb on - strand, within gluQRSat 1798.424 kb on + strand, within gluQRSat 1798.467 kb on + strand, within gluQRSat 1798.468 kb on - strand, within gluQRSat 1798.538 kb on - strandat 1798.539 kb on + strandat 1798.539 kb on + strandat 1798.539 kb on + strandat 1798.539 kb on + strandat 1798.539 kb on + strandat 1798.539 kb on + strandat 1798.540 kb on - strandat 1798.540 kb on - strandat 1798.540 kb on - strandat 1798.540 kb on - strandat 1798.540 kb on - strandat 1798.600 kb on - strandat 1798.620 kb on - strandat 1798.620 kb on - strandat 1798.798 kb on + strand, within dksAat 1798.894 kb on + strand, within dksAat 1798.894 kb on + strand, within dksAat 1798.895 kb on - strand, within dksAat 1798.895 kb on - strand, within dksAat 1798.895 kb on - strand, within dksAat 1798.895 kb on - strand, within dksAat 1798.901 kb on - strand, within dksAat 1798.934 kb on - strand, within dksAat 1798.986 kb on + strand, within dksAat 1798.987 kb on - strand, within dksAat 1799.139 kb on - strandat 1799.143 kb on - strandat 1799.143 kb on - strandat 1799.236 kb on + strandat 1799.245 kb on - strandat 1799.248 kb on + strandat 1799.249 kb on - strandat 1799.253 kb on + strandat 1799.253 kb on + strandat 1799.256 kb on + strandat 1799.317 kb on - strandat 1799.367 kb on + strand, within sfsAat 1799.517 kb on + strand, within sfsAat 1799.561 kb on - strand, within sfsAat 1799.561 kb on - strand, within sfsAat 1799.562 kb on + strand, within sfsA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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1,796,812 + pcnB BNILDI_08970 0.45 +1.1
1,796,846 + pcnB BNILDI_08970 0.47 -1.1
1,796,979 + pcnB BNILDI_08970 0.57 -0.4
1,797,062 - pcnB BNILDI_08970 0.63 +0.3
1,797,092 - pcnB BNILDI_08970 0.65 -0.9
1,797,108 - pcnB BNILDI_08970 0.66 +2.0
1,797,443 - -1.2
1,797,448 - +0.9
1,797,600 - -0.6
1,797,603 - -0.5
1,797,655 + -1.3
1,797,912 + gluQRS BNILDI_08975 0.27 -0.3
1,798,073 - gluQRS BNILDI_08975 0.45 +0.3
1,798,108 + gluQRS BNILDI_08975 0.48 +0.5
1,798,246 + gluQRS BNILDI_08975 0.63 -0.9
1,798,258 - gluQRS BNILDI_08975 0.65 -0.3
1,798,261 - gluQRS BNILDI_08975 0.65 -0.4
1,798,265 + gluQRS BNILDI_08975 0.65 -0.5
1,798,265 + gluQRS BNILDI_08975 0.65 +0.9
1,798,266 - gluQRS BNILDI_08975 0.65 -0.6
1,798,266 - gluQRS BNILDI_08975 0.65 -0.6
1,798,424 + gluQRS BNILDI_08975 0.83 -0.3
1,798,467 + gluQRS BNILDI_08975 0.87 +0.7
1,798,468 - gluQRS BNILDI_08975 0.87 -1.1
1,798,538 - -0.0
1,798,539 + -0.3
1,798,539 + -0.2
1,798,539 + +0.7
1,798,539 + -0.4
1,798,539 + -0.2
1,798,539 + -0.1
1,798,540 - -1.3
1,798,540 - -0.4
1,798,540 - +2.2
1,798,540 - +0.1
1,798,540 - +0.7
1,798,600 - +0.1
1,798,620 - +0.1
1,798,620 - -1.7
1,798,798 + dksA BNILDI_08980 0.39 -2.4
1,798,894 + dksA BNILDI_08980 0.60 -0.6
1,798,894 + dksA BNILDI_08980 0.60 -1.4
1,798,895 - dksA BNILDI_08980 0.60 -1.1
1,798,895 - dksA BNILDI_08980 0.60 -0.9
1,798,895 - dksA BNILDI_08980 0.60 -0.2
1,798,895 - dksA BNILDI_08980 0.60 -1.3
1,798,901 - dksA BNILDI_08980 0.61 -0.4
1,798,934 - dksA BNILDI_08980 0.68 -0.9
1,798,986 + dksA BNILDI_08980 0.80 -1.9
1,798,987 - dksA BNILDI_08980 0.80 -0.6
1,799,139 - +0.3
1,799,143 - +0.7
1,799,143 - +0.2
1,799,236 + -0.2
1,799,245 - +0.4
1,799,248 + +1.2
1,799,249 - -1.2
1,799,253 + +0.1
1,799,253 + +0.3
1,799,256 + -0.4
1,799,317 - +0.0
1,799,367 + sfsA BNILDI_08985 0.16 +0.4
1,799,517 + sfsA BNILDI_08985 0.37 +0.8
1,799,561 - sfsA BNILDI_08985 0.43 +0.1
1,799,561 - sfsA BNILDI_08985 0.43 +0.5
1,799,562 + sfsA BNILDI_08985 0.44 +0.2

Or see this region's nucleotide sequence