Strain Fitness in Escherichia coli ECRC62 around BNILDI_08590

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmraZ and rsmH are separated by 1 nucleotidesrsmH and BNILDI_08595 overlap by 4 nucleotidesBNILDI_08595 and ftsI are separated by 15 nucleotides BNILDI_08585: mraZ - division/cell wall cluster transcriptional repressor MraZ, at 1,718,842 to 1,719,300 mraZ BNILDI_08590: rsmH - 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH, at 1,719,302 to 1,720,243 rsmH BNILDI_08595: BNILDI_08595 - penicillin-binding protein PBP3 FtsI, at 1,720,240 to 1,720,605 _08595 BNILDI_08600: ftsI - peptidoglycan glycosyltransferase FtsI, at 1,720,621 to 1,722,387 ftsI Position (kb) 1719 1720 1721Strain fitness (log2 ratio) -2 -1 0 1at 1718.319 kb on + strandat 1718.320 kb on - strandat 1718.324 kb on - strandat 1718.324 kb on - strandat 1718.339 kb on + strandat 1718.342 kb on + strandat 1718.348 kb on + strandat 1718.349 kb on - strandat 1718.440 kb on + strandat 1718.550 kb on + strandat 1718.551 kb on - strandat 1718.551 kb on - strandat 1718.589 kb on + strandat 1718.591 kb on + strandat 1718.659 kb on - strandat 1718.694 kb on - strandat 1718.715 kb on - strandat 1719.068 kb on + strand, within mraZat 1719.068 kb on + strand, within mraZat 1719.074 kb on + strand, within mraZat 1719.682 kb on + strand, within rsmHat 1719.886 kb on + strand, within rsmHat 1720.057 kb on + strand, within rsmHat 1720.057 kb on + strand, within rsmHat 1720.603 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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1,718,319 + -1.1
1,718,320 - -0.2
1,718,324 - -0.1
1,718,324 - -0.3
1,718,339 + +0.0
1,718,342 + -0.8
1,718,348 + -0.5
1,718,349 - +0.5
1,718,440 + +1.7
1,718,550 + -0.7
1,718,551 - -0.4
1,718,551 - -0.2
1,718,589 + -0.4
1,718,591 + +0.9
1,718,659 - -0.3
1,718,694 - +0.4
1,718,715 - -0.4
1,719,068 + mraZ BNILDI_08585 0.49 -0.6
1,719,068 + mraZ BNILDI_08585 0.49 -0.6
1,719,074 + mraZ BNILDI_08585 0.51 +0.4
1,719,682 + rsmH BNILDI_08590 0.40 -2.6
1,719,886 + rsmH BNILDI_08590 0.62 -0.6
1,720,057 + rsmH BNILDI_08590 0.80 +0.3
1,720,057 + rsmH BNILDI_08590 0.80 -0.7
1,720,603 + -0.1

Or see this region's nucleotide sequence