Strain Fitness in Escherichia coli ECRC62 around BNILDI_08425

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntapaH and apaG are separated by 6 nucleotidesapaG and rsmA are separated by 2 nucleotidesrsmA and pdxA overlap by 4 nucleotidespdxA and surA overlap by 1 nucleotides BNILDI_08415: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 1,681,064 to 1,681,906 apaH BNILDI_08420: apaG - Co2+/Mg2+ efflux protein ApaG, at 1,681,913 to 1,682,290 apaG BNILDI_08425: rsmA - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA, at 1,682,293 to 1,683,114 rsmA BNILDI_08430: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 1,683,111 to 1,684,100 pdxA BNILDI_08435: surA - peptidylprolyl isomerase SurA, at 1,684,100 to 1,685,386 surA Position (kb) 1682 1683 1684Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1681.323 kb on + strand, within apaHat 1681.323 kb on + strand, within apaHat 1681.323 kb on + strand, within apaHat 1681.323 kb on + strand, within apaHat 1681.433 kb on - strand, within apaHat 1681.445 kb on - strand, within apaHat 1681.609 kb on + strand, within apaHat 1681.667 kb on - strand, within apaHat 1681.730 kb on + strand, within apaHat 1681.731 kb on - strand, within apaHat 1681.831 kb on + strandat 1681.844 kb on - strandat 1681.870 kb on - strandat 1681.896 kb on + strandat 1681.896 kb on + strandat 1681.897 kb on - strandat 1681.897 kb on - strandat 1681.907 kb on - strandat 1681.914 kb on + strandat 1682.047 kb on - strand, within apaGat 1682.197 kb on + strand, within apaGat 1682.266 kb on + strandat 1682.295 kb on - strandat 1682.416 kb on - strand, within rsmAat 1682.636 kb on - strand, within rsmAat 1682.636 kb on - strand, within rsmAat 1682.768 kb on + strand, within rsmAat 1683.249 kb on - strand, within pdxAat 1683.283 kb on - strand, within pdxAat 1683.449 kb on + strand, within pdxAat 1683.543 kb on - strand, within pdxAat 1683.814 kb on + strand, within pdxAat 1683.814 kb on + strand, within pdxAat 1683.814 kb on + strand, within pdxAat 1683.815 kb on - strand, within pdxAat 1683.815 kb on - strand, within pdxAat 1683.815 kb on - strand, within pdxAat 1683.876 kb on + strand, within pdxAat 1683.877 kb on - strand, within pdxAat 1683.877 kb on - strand, within pdxAat 1683.925 kb on + strand, within pdxAat 1683.925 kb on + strand, within pdxAat 1683.925 kb on + strand, within pdxAat 1683.926 kb on - strand, within pdxA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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1,681,323 + apaH BNILDI_08415 0.31 +1.4
1,681,323 + apaH BNILDI_08415 0.31 -0.8
1,681,323 + apaH BNILDI_08415 0.31 -1.6
1,681,323 + apaH BNILDI_08415 0.31 +2.4
1,681,433 - apaH BNILDI_08415 0.44 +0.4
1,681,445 - apaH BNILDI_08415 0.45 -0.4
1,681,609 + apaH BNILDI_08415 0.65 -3.0
1,681,667 - apaH BNILDI_08415 0.72 +2.4
1,681,730 + apaH BNILDI_08415 0.79 +0.2
1,681,731 - apaH BNILDI_08415 0.79 -0.4
1,681,831 + +0.1
1,681,844 - +0.8
1,681,870 - -0.9
1,681,896 + -0.4
1,681,896 + -1.2
1,681,897 - +0.4
1,681,897 - -0.6
1,681,907 - -1.1
1,681,914 + -0.8
1,682,047 - apaG BNILDI_08420 0.35 +0.1
1,682,197 + apaG BNILDI_08420 0.75 +0.7
1,682,266 + -1.0
1,682,295 - +0.8
1,682,416 - rsmA BNILDI_08425 0.15 +0.4
1,682,636 - rsmA BNILDI_08425 0.42 +0.3
1,682,636 - rsmA BNILDI_08425 0.42 -1.9
1,682,768 + rsmA BNILDI_08425 0.58 +0.1
1,683,249 - pdxA BNILDI_08430 0.14 +0.6
1,683,283 - pdxA BNILDI_08430 0.17 +0.2
1,683,449 + pdxA BNILDI_08430 0.34 -1.3
1,683,543 - pdxA BNILDI_08430 0.44 +0.1
1,683,814 + pdxA BNILDI_08430 0.71 +0.1
1,683,814 + pdxA BNILDI_08430 0.71 +0.1
1,683,814 + pdxA BNILDI_08430 0.71 -2.9
1,683,815 - pdxA BNILDI_08430 0.71 -0.4
1,683,815 - pdxA BNILDI_08430 0.71 -0.1
1,683,815 - pdxA BNILDI_08430 0.71 +0.7
1,683,876 + pdxA BNILDI_08430 0.77 +0.7
1,683,877 - pdxA BNILDI_08430 0.77 -0.3
1,683,877 - pdxA BNILDI_08430 0.77 -1.2
1,683,925 + pdxA BNILDI_08430 0.82 +0.4
1,683,925 + pdxA BNILDI_08430 0.82 -0.7
1,683,925 + pdxA BNILDI_08430 0.82 -0.9
1,683,926 - pdxA BNILDI_08430 0.82 -0.9

Or see this region's nucleotide sequence