Experiment: Bas05
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pmbA and cybC are separated by 182 nucleotides cybC and nrdG are separated by 44 nucleotides nrdG and nrdD are separated by 157 nucleotides
BNILDI_07325: pmbA - metalloprotease PmbA, at 1,459,915 to 1,461,267
pmbA
BNILDI_07330: cybC - cytochrome b562, at 1,461,450 to 1,461,836
cybC
BNILDI_07335: nrdG - anaerobic ribonucleoside-triphosphate reductase-activating protein, at 1,461,881 to 1,462,345
nrdG
BNILDI_07340: nrdD - anaerobic ribonucleoside-triphosphate reductase, at 1,462,503 to 1,464,641
nrdD
Position (kb)
1461
1462 Strain fitness (log2 ratio)
-1
0
1
2 at 1460.493 kb on - strand, within pmbA at 1460.493 kb on - strand, within pmbA at 1460.865 kb on + strand, within pmbA at 1460.865 kb on + strand, within pmbA at 1460.869 kb on - strand, within pmbA at 1460.869 kb on - strand, within pmbA at 1460.872 kb on - strand, within pmbA at 1460.909 kb on - strand, within pmbA at 1461.158 kb on + strand at 1461.159 kb on - strand at 1461.165 kb on + strand at 1461.165 kb on + strand at 1461.166 kb on - strand at 1461.178 kb on - strand at 1461.213 kb on + strand at 1461.213 kb on + strand at 1461.216 kb on + strand at 1461.217 kb on - strand at 1461.218 kb on + strand at 1461.218 kb on + strand at 1461.219 kb on - strand at 1461.219 kb on - strand at 1461.219 kb on - strand at 1461.371 kb on + strand at 1461.429 kb on - strand at 1461.456 kb on - strand at 1461.456 kb on - strand at 1461.467 kb on - strand at 1461.470 kb on + strand at 1461.665 kb on + strand, within cybC at 1461.666 kb on - strand, within cybC at 1461.828 kb on + strand at 1461.879 kb on + strand at 1461.880 kb on - strand at 1461.880 kb on - strand at 1461.880 kb on - strand at 1461.880 kb on - strand at 1461.880 kb on - strand at 1461.880 kb on - strand at 1462.080 kb on - strand, within nrdG at 1462.326 kb on + strand at 1462.326 kb on + strand at 1462.326 kb on + strand at 1462.329 kb on + strand at 1462.329 kb on + strand at 1462.330 kb on - strand at 1462.330 kb on - strand at 1462.339 kb on - strand at 1462.347 kb on - strand at 1462.361 kb on - strand at 1462.509 kb on + strand at 1462.545 kb on - strand at 1462.593 kb on + strand at 1462.593 kb on + strand at 1462.593 kb on + strand at 1462.594 kb on - strand at 1462.594 kb on - strand at 1462.695 kb on - strand at 1462.708 kb on + strand at 1462.741 kb on + strand, within nrdD at 1462.742 kb on - strand, within nrdD at 1462.742 kb on - strand, within nrdD at 1462.747 kb on - strand, within nrdD at 1462.763 kb on - strand, within nrdD
Per-strain Table
Position Strand Gene LocusTag Fraction Bas05 remove 1,460,493 - pmbA BNILDI_07325 0.43 +1.0 1,460,493 - pmbA BNILDI_07325 0.43 -0.7 1,460,865 + pmbA BNILDI_07325 0.70 +0.1 1,460,865 + pmbA BNILDI_07325 0.70 +0.8 1,460,869 - pmbA BNILDI_07325 0.71 -0.1 1,460,869 - pmbA BNILDI_07325 0.71 -1.2 1,460,872 - pmbA BNILDI_07325 0.71 +0.5 1,460,909 - pmbA BNILDI_07325 0.73 +2.0 1,461,158 + +1.2 1,461,159 - -1.3 1,461,165 + +1.5 1,461,165 + +0.5 1,461,166 - +0.0 1,461,178 - -0.1 1,461,213 + +0.4 1,461,213 + -0.6 1,461,216 + +2.0 1,461,217 - +0.4 1,461,218 + +0.1 1,461,218 + -0.4 1,461,219 - +1.7 1,461,219 - -0.4 1,461,219 - +1.4 1,461,371 + -1.4 1,461,429 - -0.6 1,461,456 - +0.4 1,461,456 - +0.1 1,461,467 - -0.4 1,461,470 + +0.3 1,461,665 + cybC BNILDI_07330 0.56 -0.1 1,461,666 - cybC BNILDI_07330 0.56 +0.8 1,461,828 + +0.1 1,461,879 + -0.3 1,461,880 - -0.2 1,461,880 - +1.3 1,461,880 - +0.4 1,461,880 - -1.1 1,461,880 - -0.9 1,461,880 - +0.6 1,462,080 - nrdG BNILDI_07335 0.43 -0.4 1,462,326 + +0.5 1,462,326 + -0.0 1,462,326 + +0.3 1,462,329 + -0.8 1,462,329 + -1.2 1,462,330 - +0.1 1,462,330 - +0.1 1,462,339 - -0.1 1,462,347 - +0.2 1,462,361 - +0.2 1,462,509 + +0.9 1,462,545 - -0.1 1,462,593 + +0.7 1,462,593 + +0.2 1,462,593 + -0.0 1,462,594 - -0.5 1,462,594 - +0.1 1,462,695 - +0.7 1,462,708 + +0.5 1,462,741 + nrdD BNILDI_07340 0.11 +1.6 1,462,742 - nrdD BNILDI_07340 0.11 -0.5 1,462,742 - nrdD BNILDI_07340 0.11 +0.9 1,462,747 - nrdD BNILDI_07340 0.11 +0.7 1,462,763 - nrdD BNILDI_07340 0.12 +0.6
Or see this region's nucleotide sequence