Strain Fitness in Escherichia coli ECRC62 around BNILDI_04980

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyifL and dapF are separated by 36 nucleotidesdapF and yigA overlap by 4 nucleotidesyigA and xerC overlap by 4 nucleotidesxerC and yigB overlap by 1 nucleotides BNILDI_04970: yifL - Uncharacterized lipoprotein YifL, at 984,885 to 985,088 yifL BNILDI_04975: dapF - diaminopimelate epimerase, at 985,125 to 985,949 dapF BNILDI_04980: yigA - Uncharacterized protein YigA, at 985,946 to 986,653 yigA BNILDI_04985: xerC - tyrosine recombinase XerC, at 986,650 to 987,546 xerC BNILDI_04990: yigB - 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YigB, at 987,546 to 988,262 yigB Position (kb) 985 986 987Strain fitness (log2 ratio) -2 -1 0 1 2at 984.964 kb on - strand, within yifLat 984.964 kb on - strand, within yifLat 984.980 kb on + strand, within yifLat 985.083 kb on + strandat 985.083 kb on + strandat 985.945 kb on + strandat 985.945 kb on + strandat 985.945 kb on + strandat 986.131 kb on + strand, within yigAat 986.138 kb on - strand, within yigAat 986.138 kb on - strand, within yigAat 986.211 kb on + strand, within yigAat 986.212 kb on - strand, within yigAat 986.216 kb on + strand, within yigAat 986.257 kb on + strand, within yigAat 986.257 kb on + strand, within yigAat 986.430 kb on - strand, within yigAat 986.469 kb on + strand, within yigAat 986.470 kb on - strand, within yigAat 986.546 kb on - strand, within yigAat 986.592 kb on + strandat 986.672 kb on + strandat 986.684 kb on + strandat 986.684 kb on + strandat 986.684 kb on + strandat 987.003 kb on + strand, within xerCat 987.396 kb on + strand, within xerCat 987.509 kb on - strandat 987.609 kb on + strandat 987.609 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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984,964 - yifL BNILDI_04970 0.39 -0.8
984,964 - yifL BNILDI_04970 0.39 -0.4
984,980 + yifL BNILDI_04970 0.47 -1.8
985,083 + +1.0
985,083 + -0.5
985,945 + -1.1
985,945 + -0.0
985,945 + +0.0
986,131 + yigA BNILDI_04980 0.26 +0.1
986,138 - yigA BNILDI_04980 0.27 -1.6
986,138 - yigA BNILDI_04980 0.27 +1.5
986,211 + yigA BNILDI_04980 0.37 +1.7
986,212 - yigA BNILDI_04980 0.38 -2.3
986,216 + yigA BNILDI_04980 0.38 +0.2
986,257 + yigA BNILDI_04980 0.44 -0.2
986,257 + yigA BNILDI_04980 0.44 +0.1
986,430 - yigA BNILDI_04980 0.68 +1.8
986,469 + yigA BNILDI_04980 0.74 +0.4
986,470 - yigA BNILDI_04980 0.74 +1.5
986,546 - yigA BNILDI_04980 0.85 -0.8
986,592 + -0.3
986,672 + +0.3
986,684 + -0.4
986,684 + -1.0
986,684 + -1.6
987,003 + xerC BNILDI_04985 0.39 -0.3
987,396 + xerC BNILDI_04985 0.83 -0.7
987,509 - +1.2
987,609 + +0.8
987,609 + -0.1

Or see this region's nucleotide sequence