Strain Fitness in Escherichia coli ECRC62 around BNILDI_03985

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaG and rfaQ overlap by 4 nucleotidesrfaQ and waaA are separated by 412 nucleotides BNILDI_03980: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 787,600 to 788,724 rfaG BNILDI_03985: rfaQ - lipopolysaccharide core heptosyltransferase RfaQ, at 788,721 to 789,779 rfaQ BNILDI_03990: waaA - lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase, at 790,192 to 791,469 waaA Position (kb) 788 789 790Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 787.924 kb on + strand, within rfaGat 787.924 kb on + strand, within rfaGat 788.032 kb on + strand, within rfaGat 788.261 kb on + strand, within rfaGat 788.523 kb on - strand, within rfaGat 788.659 kb on - strandat 788.700 kb on + strandat 788.804 kb on - strandat 788.804 kb on - strandat 788.940 kb on - strand, within rfaQat 788.940 kb on - strand, within rfaQat 789.148 kb on - strand, within rfaQat 789.148 kb on - strand, within rfaQat 789.227 kb on - strand, within rfaQat 789.345 kb on + strand, within rfaQat 789.346 kb on - strand, within rfaQat 789.350 kb on + strand, within rfaQat 789.459 kb on + strand, within rfaQat 789.476 kb on + strand, within rfaQat 789.476 kb on + strand, within rfaQat 789.477 kb on - strand, within rfaQat 789.477 kb on - strand, within rfaQat 789.534 kb on + strand, within rfaQat 789.726 kb on - strandat 789.897 kb on - strandat 789.897 kb on - strandat 789.934 kb on + strandat 790.030 kb on - strandat 790.106 kb on - strandat 790.109 kb on + strandat 790.151 kb on + strandat 790.175 kb on + strandat 790.184 kb on + strandat 790.188 kb on + strandat 790.191 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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787,924 + rfaG BNILDI_03980 0.29 -2.6
787,924 + rfaG BNILDI_03980 0.29 -1.0
788,032 + rfaG BNILDI_03980 0.38 -4.5
788,261 + rfaG BNILDI_03980 0.59 -2.1
788,523 - rfaG BNILDI_03980 0.82 -2.1
788,659 - +0.9
788,700 + -0.7
788,804 - +0.5
788,804 - +0.1
788,940 - rfaQ BNILDI_03985 0.21 +0.2
788,940 - rfaQ BNILDI_03985 0.21 -1.7
789,148 - rfaQ BNILDI_03985 0.40 -2.4
789,148 - rfaQ BNILDI_03985 0.40 -1.6
789,227 - rfaQ BNILDI_03985 0.48 -1.1
789,345 + rfaQ BNILDI_03985 0.59 -2.4
789,346 - rfaQ BNILDI_03985 0.59 +0.1
789,350 + rfaQ BNILDI_03985 0.59 +0.5
789,459 + rfaQ BNILDI_03985 0.70 +0.5
789,476 + rfaQ BNILDI_03985 0.71 -2.2
789,476 + rfaQ BNILDI_03985 0.71 -3.0
789,477 - rfaQ BNILDI_03985 0.71 -2.6
789,477 - rfaQ BNILDI_03985 0.71 -0.1
789,534 + rfaQ BNILDI_03985 0.77 +1.2
789,726 - -0.3
789,897 - +0.1
789,897 - +0.6
789,934 + -1.9
790,030 - -0.1
790,106 - -1.4
790,109 + -1.2
790,151 + -1.9
790,175 + -0.3
790,184 + -0.7
790,188 + -0.1
790,191 + -2.2

Or see this region's nucleotide sequence