Strain Fitness in Escherichia coli ECRC62 around BNILDI_03975

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwaaO and rfaP are separated by 15 nucleotidesrfaP and rfaG overlap by 8 nucleotides BNILDI_03970: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 785,778 to 786,794 waaO BNILDI_03975: rfaP - lipopolysaccharide core heptose(I) kinase RfaP, at 786,810 to 787,607 rfaP BNILDI_03980: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 787,600 to 788,724 rfaG Position (kb) 786 787 788Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 786.227 kb on + strand, within waaOat 786.227 kb on + strand, within waaOat 786.470 kb on + strand, within waaOat 786.470 kb on + strand, within waaOat 786.500 kb on + strand, within waaOat 786.590 kb on + strand, within waaOat 786.777 kb on - strandat 786.919 kb on + strand, within rfaPat 787.116 kb on - strand, within rfaPat 787.188 kb on - strand, within rfaPat 787.274 kb on + strand, within rfaPat 787.331 kb on - strand, within rfaPat 787.621 kb on + strandat 787.655 kb on - strandat 787.665 kb on + strandat 787.666 kb on - strandat 787.674 kb on - strandat 787.924 kb on + strand, within rfaGat 787.924 kb on + strand, within rfaGat 788.032 kb on + strand, within rfaGat 788.261 kb on + strand, within rfaGat 788.523 kb on - strand, within rfaG

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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786,227 + waaO BNILDI_03970 0.44 -1.6
786,227 + waaO BNILDI_03970 0.44 -3.5
786,470 + waaO BNILDI_03970 0.68 -2.5
786,470 + waaO BNILDI_03970 0.68 -1.1
786,500 + waaO BNILDI_03970 0.71 -3.3
786,590 + waaO BNILDI_03970 0.80 -3.1
786,777 - -3.9
786,919 + rfaP BNILDI_03975 0.14 +0.5
787,116 - rfaP BNILDI_03975 0.38 +0.9
787,188 - rfaP BNILDI_03975 0.47 -0.1
787,274 + rfaP BNILDI_03975 0.58 -0.6
787,331 - rfaP BNILDI_03975 0.65 -1.8
787,621 + +1.5
787,655 - -0.3
787,665 + -0.9
787,666 - -1.2
787,674 - -1.4
787,924 + rfaG BNILDI_03980 0.29 -2.6
787,924 + rfaG BNILDI_03980 0.29 -1.0
788,032 + rfaG BNILDI_03980 0.38 -4.5
788,261 + rfaG BNILDI_03980 0.59 -2.1
788,523 - rfaG BNILDI_03980 0.82 -2.1

Or see this region's nucleotide sequence