Strain Fitness in Escherichia coli ECRC62 around BNILDI_03965

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaJ and rfaY are separated by 25 nucleotidesrfaY and rfaJ are separated by 9 nucleotidesrfaJ and waaO are separated by 16 nucleotides BNILDI_03955: rfaJ - UDP-galactose--(galactosyl) LPS alpha1,2-galactosyltransferase, at 783,013 to 784,038 rfaJ BNILDI_03960: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 784,064 to 784,756 rfaY BNILDI_03965: rfaJ - lipopolysaccharide 1,2-glucosyltransferase, at 784,766 to 785,761 rfaJ BNILDI_03970: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 785,778 to 786,794 waaO Position (kb) 784 785 786Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 783.919 kb on + strand, within rfaJat 784.387 kb on - strand, within rfaYat 784.412 kb on - strand, within rfaYat 784.516 kb on - strand, within rfaYat 784.756 kb on + strandat 784.809 kb on - strandat 784.840 kb on - strandat 784.963 kb on + strand, within rfaJat 785.030 kb on - strand, within rfaJat 785.296 kb on + strand, within rfaJat 786.227 kb on + strand, within waaOat 786.227 kb on + strand, within waaOat 786.470 kb on + strand, within waaOat 786.470 kb on + strand, within waaOat 786.500 kb on + strand, within waaOat 786.590 kb on + strand, within waaO

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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783,919 + rfaJ BNILDI_03955 0.88 -1.1
784,387 - rfaY BNILDI_03960 0.47 -0.2
784,412 - rfaY BNILDI_03960 0.50 -0.9
784,516 - rfaY BNILDI_03960 0.65 -0.4
784,756 + -3.2
784,809 - -2.7
784,840 - -3.0
784,963 + rfaJ BNILDI_03965 0.20 -0.3
785,030 - rfaJ BNILDI_03965 0.27 -4.8
785,296 + rfaJ BNILDI_03965 0.53 -2.3
786,227 + waaO BNILDI_03970 0.44 -1.6
786,227 + waaO BNILDI_03970 0.44 -3.5
786,470 + waaO BNILDI_03970 0.68 -2.5
786,470 + waaO BNILDI_03970 0.68 -1.1
786,500 + waaO BNILDI_03970 0.71 -3.3
786,590 + waaO BNILDI_03970 0.80 -3.1

Or see this region's nucleotide sequence