Strain Fitness in Escherichia coli ECRC62 around BNILDI_03955

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBNILDI_03950 and rfaJ are separated by 84 nucleotidesrfaJ and rfaY are separated by 25 nucleotidesrfaY and rfaJ are separated by 9 nucleotides BNILDI_03950: BNILDI_03950 - beta1,3-glucosyltransferase, at 781,945 to 782,928 _03950 BNILDI_03955: rfaJ - UDP-galactose--(galactosyl) LPS alpha1,2-galactosyltransferase, at 783,013 to 784,038 rfaJ BNILDI_03960: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 784,064 to 784,756 rfaY BNILDI_03965: rfaJ - lipopolysaccharide 1,2-glucosyltransferase, at 784,766 to 785,761 rfaJ Position (kb) 783 784 785Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 782.207 kb on - strand, within BNILDI_03950at 782.319 kb on + strand, within BNILDI_03950at 782.363 kb on + strand, within BNILDI_03950at 782.442 kb on + strand, within BNILDI_03950at 782.508 kb on + strand, within BNILDI_03950at 782.574 kb on - strand, within BNILDI_03950at 782.629 kb on - strand, within BNILDI_03950at 782.664 kb on + strand, within BNILDI_03950at 782.664 kb on + strand, within BNILDI_03950at 782.665 kb on - strand, within BNILDI_03950at 783.245 kb on + strand, within rfaJat 783.379 kb on - strand, within rfaJat 783.405 kb on + strand, within rfaJat 783.405 kb on + strand, within rfaJat 783.405 kb on + strand, within rfaJat 783.406 kb on - strand, within rfaJat 783.493 kb on + strand, within rfaJat 783.497 kb on - strand, within rfaJat 783.532 kb on + strand, within rfaJat 783.666 kb on - strand, within rfaJat 783.919 kb on + strand, within rfaJat 784.387 kb on - strand, within rfaYat 784.412 kb on - strand, within rfaYat 784.516 kb on - strand, within rfaYat 784.756 kb on + strandat 784.809 kb on - strandat 784.840 kb on - strandat 784.963 kb on + strand, within rfaJat 785.030 kb on - strand, within rfaJ

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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782,207 - BNILDI_03950 0.27 -1.9
782,319 + BNILDI_03950 0.38 -4.6
782,363 + BNILDI_03950 0.42 -2.1
782,442 + BNILDI_03950 0.51 -2.1
782,508 + BNILDI_03950 0.57 -1.8
782,574 - BNILDI_03950 0.64 -1.6
782,629 - BNILDI_03950 0.70 -3.7
782,664 + BNILDI_03950 0.73 -2.4
782,664 + BNILDI_03950 0.73 -2.7
782,665 - BNILDI_03950 0.73 -2.5
783,245 + rfaJ BNILDI_03955 0.23 -0.8
783,379 - rfaJ BNILDI_03955 0.36 +0.7
783,405 + rfaJ BNILDI_03955 0.38 -3.4
783,405 + rfaJ BNILDI_03955 0.38 -3.3
783,405 + rfaJ BNILDI_03955 0.38 -3.3
783,406 - rfaJ BNILDI_03955 0.38 -0.6
783,493 + rfaJ BNILDI_03955 0.47 -3.6
783,497 - rfaJ BNILDI_03955 0.47 -0.1
783,532 + rfaJ BNILDI_03955 0.51 -0.7
783,666 - rfaJ BNILDI_03955 0.64 -0.8
783,919 + rfaJ BNILDI_03955 0.88 -1.1
784,387 - rfaY BNILDI_03960 0.47 -0.2
784,412 - rfaY BNILDI_03960 0.50 -0.9
784,516 - rfaY BNILDI_03960 0.65 -0.4
784,756 + -3.2
784,809 - -2.7
784,840 - -3.0
784,963 + rfaJ BNILDI_03965 0.20 -0.3
785,030 - rfaJ BNILDI_03965 0.27 -4.8

Or see this region's nucleotide sequence