Experiment: Bas05
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tdcG and tdcF are separated by 71 nucleotides tdcF and tdcE are separated by 13 nucleotides tdcE and tdcD are separated by 33 nucleotides
BNILDI_01320: tdcG - L-serine ammonia-lyase, at 257,913 to 259,277
tdcG
BNILDI_01325: tdcF - enamine/imine deaminase, at 259,349 to 259,738
tdcF
BNILDI_01330: tdcE - 2-ketobutyrate formate-lyase/pyruvate formate-lyase, at 259,752 to 262,046
tdcE
BNILDI_01335: tdcD - propionate kinase, at 262,080 to 263,288
tdcD
Position (kb)
259
260
261
262
263 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 258.847 kb on + strand, within tdcG at 258.847 kb on + strand, within tdcG at 258.848 kb on - strand, within tdcG at 258.848 kb on - strand, within tdcG at 258.850 kb on + strand, within tdcG at 258.851 kb on - strand, within tdcG at 258.851 kb on - strand, within tdcG at 258.857 kb on + strand, within tdcG at 258.861 kb on + strand, within tdcG at 258.939 kb on - strand, within tdcG at 259.011 kb on + strand, within tdcG at 259.019 kb on + strand, within tdcG at 259.020 kb on - strand, within tdcG at 259.129 kb on + strand, within tdcG at 259.145 kb on + strand at 259.146 kb on - strand at 259.165 kb on - strand at 259.165 kb on - strand at 259.190 kb on + strand at 259.271 kb on + strand at 259.350 kb on + strand at 259.351 kb on - strand at 259.357 kb on + strand at 259.358 kb on - strand at 259.358 kb on - strand at 259.467 kb on + strand, within tdcF at 259.467 kb on + strand, within tdcF at 259.467 kb on + strand, within tdcF at 259.467 kb on + strand, within tdcF at 259.468 kb on - strand, within tdcF at 259.690 kb on - strand, within tdcF at 259.738 kb on + strand at 259.738 kb on + strand at 259.739 kb on - strand at 259.767 kb on - strand at 259.958 kb on - strand at 259.988 kb on + strand, within tdcE at 259.989 kb on - strand, within tdcE at 260.038 kb on + strand, within tdcE at 260.060 kb on - strand, within tdcE at 260.216 kb on + strand, within tdcE at 260.279 kb on + strand, within tdcE at 260.314 kb on - strand, within tdcE at 260.411 kb on - strand, within tdcE at 260.411 kb on - strand, within tdcE at 260.468 kb on + strand, within tdcE at 260.516 kb on - strand, within tdcE at 260.522 kb on - strand, within tdcE at 260.536 kb on + strand, within tdcE at 260.563 kb on + strand, within tdcE at 260.563 kb on + strand, within tdcE at 260.813 kb on + strand, within tdcE at 260.837 kb on - strand, within tdcE at 260.896 kb on - strand, within tdcE at 260.924 kb on + strand, within tdcE at 260.993 kb on + strand, within tdcE at 261.074 kb on + strand, within tdcE at 261.074 kb on + strand, within tdcE at 261.088 kb on - strand, within tdcE at 261.175 kb on + strand, within tdcE at 261.180 kb on - strand, within tdcE at 261.460 kb on + strand, within tdcE at 261.472 kb on + strand, within tdcE at 261.480 kb on + strand, within tdcE at 261.517 kb on + strand, within tdcE at 261.528 kb on + strand, within tdcE at 261.588 kb on + strand, within tdcE at 261.588 kb on + strand, within tdcE at 261.589 kb on - strand, within tdcE at 261.678 kb on - strand, within tdcE at 261.683 kb on + strand, within tdcE at 261.705 kb on + strand, within tdcE at 261.767 kb on + strand, within tdcE at 261.768 kb on - strand, within tdcE at 261.861 kb on + strand at 261.933 kb on - strand at 261.934 kb on + strand at 261.935 kb on - strand at 261.935 kb on - strand at 261.938 kb on + strand at 261.939 kb on - strand at 261.948 kb on - strand at 261.989 kb on + strand at 262.035 kb on - strand at 262.076 kb on + strand at 262.114 kb on - strand at 262.325 kb on + strand, within tdcD at 262.475 kb on + strand, within tdcD at 262.479 kb on + strand, within tdcD at 262.479 kb on + strand, within tdcD at 262.497 kb on - strand, within tdcD at 262.628 kb on + strand, within tdcD at 262.629 kb on - strand, within tdcD at 262.747 kb on + strand, within tdcD at 262.747 kb on + strand, within tdcD at 262.934 kb on + strand, within tdcD at 262.935 kb on - strand, within tdcD
Per-strain Table
Position Strand Gene LocusTag Fraction Bas05 remove 258,847 + tdcG BNILDI_01320 0.68 -0.2 258,847 + tdcG BNILDI_01320 0.68 -0.3 258,848 - tdcG BNILDI_01320 0.68 -1.5 258,848 - tdcG BNILDI_01320 0.68 -1.8 258,850 + tdcG BNILDI_01320 0.69 -1.3 258,851 - tdcG BNILDI_01320 0.69 +0.8 258,851 - tdcG BNILDI_01320 0.69 -0.8 258,857 + tdcG BNILDI_01320 0.69 -0.6 258,861 + tdcG BNILDI_01320 0.69 +0.8 258,939 - tdcG BNILDI_01320 0.75 +0.6 259,011 + tdcG BNILDI_01320 0.80 +0.4 259,019 + tdcG BNILDI_01320 0.81 +0.8 259,020 - tdcG BNILDI_01320 0.81 +0.6 259,129 + tdcG BNILDI_01320 0.89 +0.4 259,145 + -0.8 259,146 - +0.1 259,165 - -1.7 259,165 - -0.6 259,190 + -0.5 259,271 + -1.6 259,350 + -0.3 259,351 - +0.3 259,357 + -1.1 259,358 - +1.2 259,358 - -0.6 259,467 + tdcF BNILDI_01325 0.30 -1.0 259,467 + tdcF BNILDI_01325 0.30 +1.7 259,467 + tdcF BNILDI_01325 0.30 -0.6 259,467 + tdcF BNILDI_01325 0.30 +0.1 259,468 - tdcF BNILDI_01325 0.31 -0.5 259,690 - tdcF BNILDI_01325 0.87 -0.7 259,738 + +1.2 259,738 + -0.6 259,739 - +0.3 259,767 - -0.2 259,958 - +0.8 259,988 + tdcE BNILDI_01330 0.10 +0.5 259,989 - tdcE BNILDI_01330 0.10 -1.4 260,038 + tdcE BNILDI_01330 0.12 +0.2 260,060 - tdcE BNILDI_01330 0.13 +2.2 260,216 + tdcE BNILDI_01330 0.20 -1.2 260,279 + tdcE BNILDI_01330 0.23 +0.9 260,314 - tdcE BNILDI_01330 0.24 -0.5 260,411 - tdcE BNILDI_01330 0.29 +1.1 260,411 - tdcE BNILDI_01330 0.29 -0.3 260,468 + tdcE BNILDI_01330 0.31 +0.0 260,516 - tdcE BNILDI_01330 0.33 -0.1 260,522 - tdcE BNILDI_01330 0.34 -0.7 260,536 + tdcE BNILDI_01330 0.34 +0.2 260,563 + tdcE BNILDI_01330 0.35 -0.6 260,563 + tdcE BNILDI_01330 0.35 +1.1 260,813 + tdcE BNILDI_01330 0.46 +0.3 260,837 - tdcE BNILDI_01330 0.47 -2.1 260,896 - tdcE BNILDI_01330 0.50 -0.3 260,924 + tdcE BNILDI_01330 0.51 -1.2 260,993 + tdcE BNILDI_01330 0.54 -0.6 261,074 + tdcE BNILDI_01330 0.58 -0.2 261,074 + tdcE BNILDI_01330 0.58 +0.3 261,088 - tdcE BNILDI_01330 0.58 -0.9 261,175 + tdcE BNILDI_01330 0.62 +0.3 261,180 - tdcE BNILDI_01330 0.62 +0.1 261,460 + tdcE BNILDI_01330 0.74 -0.8 261,472 + tdcE BNILDI_01330 0.75 +0.8 261,480 + tdcE BNILDI_01330 0.75 +0.7 261,517 + tdcE BNILDI_01330 0.77 -1.3 261,528 + tdcE BNILDI_01330 0.77 +1.5 261,588 + tdcE BNILDI_01330 0.80 -0.0 261,588 + tdcE BNILDI_01330 0.80 -0.9 261,589 - tdcE BNILDI_01330 0.80 +1.0 261,678 - tdcE BNILDI_01330 0.84 -0.5 261,683 + tdcE BNILDI_01330 0.84 -0.9 261,705 + tdcE BNILDI_01330 0.85 -0.8 261,767 + tdcE BNILDI_01330 0.88 -0.2 261,768 - tdcE BNILDI_01330 0.88 -1.4 261,861 + +0.1 261,933 - -0.4 261,934 + +0.3 261,935 - -0.3 261,935 - +1.9 261,938 + -0.2 261,939 - -0.3 261,948 - +0.2 261,989 + +1.2 262,035 - +0.8 262,076 + +0.1 262,114 - -0.5 262,325 + tdcD BNILDI_01335 0.20 -0.3 262,475 + tdcD BNILDI_01335 0.33 -1.5 262,479 + tdcD BNILDI_01335 0.33 +0.5 262,479 + tdcD BNILDI_01335 0.33 -1.0 262,497 - tdcD BNILDI_01335 0.34 -1.1 262,628 + tdcD BNILDI_01335 0.45 -0.6 262,629 - tdcD BNILDI_01335 0.45 -0.6 262,747 + tdcD BNILDI_01335 0.55 -1.5 262,747 + tdcD BNILDI_01335 0.55 -0.7 262,934 + tdcD BNILDI_01335 0.71 -0.9 262,935 - tdcD BNILDI_01335 0.71 -0.2
Or see this region's nucleotide sequence