Experiment: Bas05
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glgS and yqiJ are separated by 268 nucleotides yqiJ and yqiK are separated by 26 nucleotides yqiK and ibsD are separated by 238 nucleotides ibsD and BNILDI_00990 are separated by 315 nucleotides BNILDI_00990 and hldE are separated by 119 nucleotides
BNILDI_00960: glgS - cell surface composition regulator GlgS, at 185,401 to 185,601
glgS
BNILDI_00965: yqiJ - Inner membrane protein YqiJ, at 185,870 to 186,499
yqiJ
BNILDI_00970: yqiK - Flotillin family inner membrane protein YqiK, at 186,526 to 188,187
yqiK
BNILDI_00980: ibsD - Small toxic protein IbsD, at 188,426 to 188,485
ibsD
BNILDI_00990: BNILDI_00990 - Type I toxin-antitoxin system Ibs family toxin, at 188,801 to 188,860
_00990
BNILDI_00995: hldE - bifunctional D-glycero-beta-D-manno-heptose-7-phosphate kinase/D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase HldE, at 188,980 to 190,413
hldE
Position (kb) 186
187
188
189 Strain fitness (log2 ratio) -1
0
1
2 at 185.554 kb on - strand, within glgS at 185.593 kb on - strand at 185.633 kb on - strand at 185.641 kb on - strand at 185.686 kb on - strand at 185.691 kb on + strand at 185.869 kb on + strand at 185.891 kb on - strand at 185.922 kb on + strand at 185.923 kb on - strand at 186.285 kb on - strand, within yqiJ at 186.666 kb on - strand at 186.725 kb on + strand, within yqiK at 186.744 kb on + strand, within yqiK at 186.745 kb on - strand, within yqiK at 186.815 kb on + strand, within yqiK at 186.819 kb on - strand, within yqiK at 186.852 kb on - strand, within yqiK at 186.852 kb on - strand, within yqiK at 186.911 kb on + strand, within yqiK at 186.911 kb on + strand, within yqiK at 186.912 kb on - strand, within yqiK at 187.077 kb on + strand, within yqiK at 187.077 kb on + strand, within yqiK at 187.185 kb on + strand, within yqiK at 187.279 kb on - strand, within yqiK at 187.473 kb on + strand, within yqiK at 187.601 kb on + strand, within yqiK at 187.786 kb on - strand, within yqiK at 187.792 kb on - strand, within yqiK at 187.840 kb on - strand, within yqiK at 187.847 kb on + strand, within yqiK at 187.848 kb on - strand, within yqiK at 187.872 kb on + strand, within yqiK at 188.181 kb on - strand at 188.186 kb on - strand at 188.197 kb on + strand at 188.234 kb on - strand at 188.480 kb on + strand at 188.480 kb on + strand at 188.535 kb on + strand at 188.571 kb on + strand at 188.579 kb on - strand at 188.579 kb on - strand at 188.579 kb on - strand at 188.624 kb on + strand at 188.786 kb on + strand at 188.786 kb on + strand at 188.800 kb on + strand at 188.842 kb on - strand, within BNILDI_00990 at 188.855 kb on + strand at 188.855 kb on + strand at 188.855 kb on + strand at 188.858 kb on + strand at 188.859 kb on - strand at 188.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas05 remove 185,554 - glgS BNILDI_00960 0.76 -0.1 185,593 - -0.9 185,633 - -0.2 185,641 - -1.0 185,686 - +0.4 185,691 + +0.3 185,869 + -0.1 185,891 - -0.1 185,922 + +0.3 185,923 - +0.3 186,285 - yqiJ BNILDI_00965 0.66 -1.3 186,666 - +1.4 186,725 + yqiK BNILDI_00970 0.12 -1.3 186,744 + yqiK BNILDI_00970 0.13 +1.2 186,745 - yqiK BNILDI_00970 0.13 -0.9 186,815 + yqiK BNILDI_00970 0.17 -0.1 186,819 - yqiK BNILDI_00970 0.18 +0.4 186,852 - yqiK BNILDI_00970 0.20 +0.3 186,852 - yqiK BNILDI_00970 0.20 -0.2 186,911 + yqiK BNILDI_00970 0.23 +0.3 186,911 + yqiK BNILDI_00970 0.23 +0.5 186,912 - yqiK BNILDI_00970 0.23 +0.0 187,077 + yqiK BNILDI_00970 0.33 -1.0 187,077 + yqiK BNILDI_00970 0.33 -0.2 187,185 + yqiK BNILDI_00970 0.40 -0.9 187,279 - yqiK BNILDI_00970 0.45 +0.1 187,473 + yqiK BNILDI_00970 0.57 +0.1 187,601 + yqiK BNILDI_00970 0.65 +0.5 187,786 - yqiK BNILDI_00970 0.76 -0.4 187,792 - yqiK BNILDI_00970 0.76 -0.0 187,840 - yqiK BNILDI_00970 0.79 +1.1 187,847 + yqiK BNILDI_00970 0.79 -0.2 187,848 - yqiK BNILDI_00970 0.80 +0.2 187,872 + yqiK BNILDI_00970 0.81 -0.2 188,181 - -0.0 188,186 - +0.3 188,197 + -0.5 188,234 - -0.6 188,480 + +0.1 188,480 + +0.1 188,535 + -0.4 188,571 + -0.0 188,579 - +0.8 188,579 - +2.4 188,579 - -0.9 188,624 + -0.3 188,786 + +0.5 188,786 + -1.0 188,800 + -0.2 188,842 - BNILDI_00990 0.68 -0.2 188,855 + -0.8 188,855 + +1.0 188,855 + +0.4 188,858 + +0.2 188,859 - -0.4 188,937 - +0.8
Or see this region's nucleotide sequence