Experiment: GB_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybcN and ninE overlap by 1 nucleotides ninE and ybcO overlap by 8 nucleotides ybcO and rusA overlap by 4 nucleotides rusA and ylcG overlap by 1 nucleotides ylcG and JDDGAC_27795 overlap by 4 nucleotides JDDGAC_27795 and JDDGAC_27800 are separated by 62 nucleotides
JDDGAC_27770: ybcN - DNA base-flipping protein YbcN, at 5,331,159 to 5,331,614
ybcN
JDDGAC_27775: ninE - Protein ninE, at 5,331,614 to 5,331,784
ninE
JDDGAC_27780: ybcO - Putative nuclease YbcO, at 5,331,777 to 5,332,067
ybcO
JDDGAC_27785: rusA - crossover junction endodeoxyribonuclease RusA, at 5,332,064 to 5,332,426
rusA
JDDGAC_27790: ylcG - Uncharacterized protein YlcG, at 5,332,426 to 5,332,563
ylcG
JDDGAC_27795: JDDGAC_27795 - Orf33, orf151, orf56, orf96, rus, orf45, orf8, orf127, and nmpC genes, at 5,332,560 to 5,332,586
_27795
JDDGAC_27800: JDDGAC_27800 - antitermination protein, at 5,332,649 to 5,333,083
_27800
Position (kb)
5332
5333 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5331.070 kb on + strand at 5331.071 kb on - strand at 5331.071 kb on - strand at 5331.232 kb on - strand, within ybcN at 5331.266 kb on + strand, within ybcN at 5331.267 kb on - strand, within ybcN at 5331.267 kb on - strand, within ybcN at 5332.086 kb on + strand at 5332.220 kb on - strand, within rusA at 5332.438 kb on - strand at 5332.438 kb on - strand at 5332.481 kb on + strand, within ylcG at 5332.484 kb on - strand, within ylcG at 5332.559 kb on + strand at 5332.559 kb on + strand at 5332.574 kb on - strand, within JDDGAC_27795 at 5332.585 kb on - strand at 5332.588 kb on - strand at 5332.699 kb on + strand, within JDDGAC_27800 at 5332.699 kb on + strand, within JDDGAC_27800 at 5332.817 kb on - strand, within JDDGAC_27800 at 5332.817 kb on - strand, within JDDGAC_27800 at 5332.868 kb on + strand, within JDDGAC_27800 at 5332.869 kb on - strand, within JDDGAC_27800 at 5332.871 kb on + strand, within JDDGAC_27800 at 5332.872 kb on - strand, within JDDGAC_27800 at 5332.930 kb on + strand, within JDDGAC_27800 at 5332.931 kb on - strand, within JDDGAC_27800 at 5332.932 kb on + strand, within JDDGAC_27800 at 5332.932 kb on + strand, within JDDGAC_27800 at 5332.932 kb on + strand, within JDDGAC_27800 at 5332.932 kb on + strand, within JDDGAC_27800 at 5332.932 kb on + strand, within JDDGAC_27800 at 5332.933 kb on - strand, within JDDGAC_27800 at 5332.933 kb on - strand, within JDDGAC_27800 at 5332.936 kb on + strand, within JDDGAC_27800 at 5332.936 kb on + strand, within JDDGAC_27800 at 5332.937 kb on - strand, within JDDGAC_27800 at 5333.101 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction GB_JK32 remove 5,331,070 + -0.8 5,331,071 - +0.6 5,331,071 - -0.5 5,331,232 - ybcN JDDGAC_27770 0.16 -2.7 5,331,266 + ybcN JDDGAC_27770 0.23 -0.4 5,331,267 - ybcN JDDGAC_27770 0.24 +3.1 5,331,267 - ybcN JDDGAC_27770 0.24 -0.7 5,332,086 + +0.4 5,332,220 - rusA JDDGAC_27785 0.43 -1.7 5,332,438 - +0.8 5,332,438 - -1.2 5,332,481 + ylcG JDDGAC_27790 0.40 -0.5 5,332,484 - ylcG JDDGAC_27790 0.42 +1.5 5,332,559 + -2.2 5,332,559 + +0.1 5,332,574 - JDDGAC_27795 0.52 +0.7 5,332,585 - +0.2 5,332,588 - -1.7 5,332,699 + JDDGAC_27800 0.11 +1.7 5,332,699 + JDDGAC_27800 0.11 -0.0 5,332,817 - JDDGAC_27800 0.39 +1.7 5,332,817 - JDDGAC_27800 0.39 +0.4 5,332,868 + JDDGAC_27800 0.50 -1.4 5,332,869 - JDDGAC_27800 0.51 +1.8 5,332,871 + JDDGAC_27800 0.51 +0.3 5,332,872 - JDDGAC_27800 0.51 -2.0 5,332,930 + JDDGAC_27800 0.65 +0.1 5,332,931 - JDDGAC_27800 0.65 -0.6 5,332,932 + JDDGAC_27800 0.65 -3.0 5,332,932 + JDDGAC_27800 0.65 -0.2 5,332,932 + JDDGAC_27800 0.65 +0.3 5,332,932 + JDDGAC_27800 0.65 +0.8 5,332,932 + JDDGAC_27800 0.65 -0.3 5,332,933 - JDDGAC_27800 0.65 -0.5 5,332,933 - JDDGAC_27800 0.65 +0.4 5,332,936 + JDDGAC_27800 0.66 +0.2 5,332,936 + JDDGAC_27800 0.66 +0.4 5,332,937 - JDDGAC_27800 0.66 -0.4 5,333,101 + -2.2
Or see this region's nucleotide sequence