Strain Fitness in Escherichia coli ECRC98 around JDDGAC_27785

Experiment: GB_JK32

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybcN and ninE overlap by 1 nucleotidesninE and ybcO overlap by 8 nucleotidesybcO and rusA overlap by 4 nucleotidesrusA and ylcG overlap by 1 nucleotidesylcG and JDDGAC_27795 overlap by 4 nucleotidesJDDGAC_27795 and JDDGAC_27800 are separated by 62 nucleotides JDDGAC_27770: ybcN - DNA base-flipping protein YbcN, at 5,331,159 to 5,331,614 ybcN JDDGAC_27775: ninE - Protein ninE, at 5,331,614 to 5,331,784 ninE JDDGAC_27780: ybcO - Putative nuclease YbcO, at 5,331,777 to 5,332,067 ybcO JDDGAC_27785: rusA - crossover junction endodeoxyribonuclease RusA, at 5,332,064 to 5,332,426 rusA JDDGAC_27790: ylcG - Uncharacterized protein YlcG, at 5,332,426 to 5,332,563 ylcG JDDGAC_27795: JDDGAC_27795 - Orf33, orf151, orf56, orf96, rus, orf45, orf8, orf127, and nmpC genes, at 5,332,560 to 5,332,586 _27795 JDDGAC_27800: JDDGAC_27800 - antitermination protein, at 5,332,649 to 5,333,083 _27800 Position (kb) 5332 5333Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 5331.070 kb on + strandat 5331.071 kb on - strandat 5331.071 kb on - strandat 5331.232 kb on - strand, within ybcNat 5331.266 kb on + strand, within ybcNat 5331.267 kb on - strand, within ybcNat 5331.267 kb on - strand, within ybcNat 5332.086 kb on + strandat 5332.220 kb on - strand, within rusAat 5332.438 kb on - strandat 5332.438 kb on - strandat 5332.481 kb on + strand, within ylcGat 5332.484 kb on - strand, within ylcGat 5332.559 kb on + strandat 5332.559 kb on + strandat 5332.574 kb on - strand, within JDDGAC_27795at 5332.585 kb on - strandat 5332.588 kb on - strandat 5332.699 kb on + strand, within JDDGAC_27800at 5332.699 kb on + strand, within JDDGAC_27800at 5332.817 kb on - strand, within JDDGAC_27800at 5332.817 kb on - strand, within JDDGAC_27800at 5332.868 kb on + strand, within JDDGAC_27800at 5332.869 kb on - strand, within JDDGAC_27800at 5332.871 kb on + strand, within JDDGAC_27800at 5332.872 kb on - strand, within JDDGAC_27800at 5332.930 kb on + strand, within JDDGAC_27800at 5332.931 kb on - strand, within JDDGAC_27800at 5332.932 kb on + strand, within JDDGAC_27800at 5332.932 kb on + strand, within JDDGAC_27800at 5332.932 kb on + strand, within JDDGAC_27800at 5332.932 kb on + strand, within JDDGAC_27800at 5332.932 kb on + strand, within JDDGAC_27800at 5332.933 kb on - strand, within JDDGAC_27800at 5332.933 kb on - strand, within JDDGAC_27800at 5332.936 kb on + strand, within JDDGAC_27800at 5332.936 kb on + strand, within JDDGAC_27800at 5332.937 kb on - strand, within JDDGAC_27800at 5333.101 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction GB_JK32
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5,331,070 + -0.8
5,331,071 - +0.6
5,331,071 - -0.5
5,331,232 - ybcN JDDGAC_27770 0.16 -2.7
5,331,266 + ybcN JDDGAC_27770 0.23 -0.4
5,331,267 - ybcN JDDGAC_27770 0.24 +3.1
5,331,267 - ybcN JDDGAC_27770 0.24 -0.7
5,332,086 + +0.4
5,332,220 - rusA JDDGAC_27785 0.43 -1.7
5,332,438 - +0.8
5,332,438 - -1.2
5,332,481 + ylcG JDDGAC_27790 0.40 -0.5
5,332,484 - ylcG JDDGAC_27790 0.42 +1.5
5,332,559 + -2.2
5,332,559 + +0.1
5,332,574 - JDDGAC_27795 0.52 +0.7
5,332,585 - +0.2
5,332,588 - -1.7
5,332,699 + JDDGAC_27800 0.11 +1.7
5,332,699 + JDDGAC_27800 0.11 -0.0
5,332,817 - JDDGAC_27800 0.39 +1.7
5,332,817 - JDDGAC_27800 0.39 +0.4
5,332,868 + JDDGAC_27800 0.50 -1.4
5,332,869 - JDDGAC_27800 0.51 +1.8
5,332,871 + JDDGAC_27800 0.51 +0.3
5,332,872 - JDDGAC_27800 0.51 -2.0
5,332,930 + JDDGAC_27800 0.65 +0.1
5,332,931 - JDDGAC_27800 0.65 -0.6
5,332,932 + JDDGAC_27800 0.65 -3.0
5,332,932 + JDDGAC_27800 0.65 -0.2
5,332,932 + JDDGAC_27800 0.65 +0.3
5,332,932 + JDDGAC_27800 0.65 +0.8
5,332,932 + JDDGAC_27800 0.65 -0.3
5,332,933 - JDDGAC_27800 0.65 -0.5
5,332,933 - JDDGAC_27800 0.65 +0.4
5,332,936 + JDDGAC_27800 0.66 +0.2
5,332,936 + JDDGAC_27800 0.66 +0.4
5,332,937 - JDDGAC_27800 0.66 -0.4
5,333,101 + -2.2

Or see this region's nucleotide sequence