Experiment: GB_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mltB and pncC are separated by 144 nucleotides pncC and recA are separated by 79 nucleotides recA and recX are separated by 67 nucleotides recX and alaS are separated by 127 nucleotides
JDDGAC_24360: mltB - lytic murein transglycosylase B, at 4,671,029 to 4,672,219
mltB
JDDGAC_24365: pncC - nicotinamide-nucleotide amidase, at 4,672,364 to 4,672,861
pncC
JDDGAC_24370: recA - recombinase RecA, at 4,672,941 to 4,674,002
recA
JDDGAC_24375: recX - recombination regulator RecX, at 4,674,070 to 4,674,570
recX
JDDGAC_24380: alaS - alanine--tRNA ligase, at 4,674,698 to 4,677,328
alaS
Position (kb)
4672
4673
4674
4675 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4672.112 kb on + strand at 4672.130 kb on + strand at 4672.130 kb on + strand at 4672.156 kb on + strand at 4672.186 kb on - strand at 4672.221 kb on + strand at 4672.222 kb on - strand at 4672.324 kb on + strand at 4672.339 kb on - strand at 4672.389 kb on + strand at 4672.529 kb on + strand, within pncC at 4672.529 kb on + strand, within pncC at 4672.530 kb on - strand, within pncC at 4672.530 kb on - strand, within pncC at 4672.530 kb on - strand, within pncC at 4672.530 kb on - strand, within pncC at 4672.534 kb on + strand, within pncC at 4672.627 kb on - strand, within pncC at 4672.655 kb on + strand, within pncC at 4672.655 kb on + strand, within pncC at 4672.656 kb on - strand, within pncC at 4672.666 kb on + strand, within pncC at 4672.667 kb on - strand, within pncC at 4672.667 kb on - strand, within pncC at 4672.667 kb on - strand, within pncC at 4672.849 kb on - strand at 4674.016 kb on + strand at 4674.051 kb on + strand at 4674.051 kb on + strand at 4674.058 kb on + strand at 4674.058 kb on + strand at 4674.058 kb on + strand at 4674.059 kb on - strand at 4674.059 kb on - strand at 4674.059 kb on - strand at 4674.100 kb on + strand at 4674.100 kb on + strand at 4674.100 kb on + strand at 4674.100 kb on + strand at 4674.100 kb on + strand at 4674.101 kb on - strand at 4674.101 kb on - strand at 4674.101 kb on - strand at 4674.101 kb on - strand at 4674.101 kb on - strand at 4674.189 kb on + strand, within recX at 4674.370 kb on - strand, within recX at 4674.515 kb on - strand, within recX at 4674.515 kb on - strand, within recX at 4674.546 kb on + strand at 4674.582 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction GB_JK32 remove 4,672,112 + -0.3 4,672,130 + -2.0 4,672,130 + +2.4 4,672,156 + -0.9 4,672,186 - -0.5 4,672,221 + +0.6 4,672,222 - -1.0 4,672,324 + +1.1 4,672,339 - +1.5 4,672,389 + -2.0 4,672,529 + pncC JDDGAC_24365 0.33 -1.0 4,672,529 + pncC JDDGAC_24365 0.33 +0.3 4,672,530 - pncC JDDGAC_24365 0.33 -1.1 4,672,530 - pncC JDDGAC_24365 0.33 +0.5 4,672,530 - pncC JDDGAC_24365 0.33 +1.6 4,672,530 - pncC JDDGAC_24365 0.33 +0.6 4,672,534 + pncC JDDGAC_24365 0.34 -0.1 4,672,627 - pncC JDDGAC_24365 0.53 +0.1 4,672,655 + pncC JDDGAC_24365 0.58 +1.2 4,672,655 + pncC JDDGAC_24365 0.58 +0.4 4,672,656 - pncC JDDGAC_24365 0.59 -1.8 4,672,666 + pncC JDDGAC_24365 0.61 -2.0 4,672,667 - pncC JDDGAC_24365 0.61 +1.0 4,672,667 - pncC JDDGAC_24365 0.61 -2.5 4,672,667 - pncC JDDGAC_24365 0.61 -0.7 4,672,849 - -1.6 4,674,016 + +0.5 4,674,051 + -2.3 4,674,051 + +1.0 4,674,058 + -2.2 4,674,058 + -0.3 4,674,058 + -0.2 4,674,059 - -0.4 4,674,059 - -2.1 4,674,059 - +2.5 4,674,100 + -2.7 4,674,100 + +0.3 4,674,100 + -1.2 4,674,100 + +0.5 4,674,100 + -0.2 4,674,101 - +0.7 4,674,101 - +1.8 4,674,101 - +0.4 4,674,101 - -3.1 4,674,101 - -1.3 4,674,189 + recX JDDGAC_24375 0.24 +2.2 4,674,370 - recX JDDGAC_24375 0.60 +0.6 4,674,515 - recX JDDGAC_24375 0.89 -1.8 4,674,515 - recX JDDGAC_24375 0.89 +0.6 4,674,546 + -1.9 4,674,582 - -1.4
Or see this region's nucleotide sequence