Experiment: GB_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pulJ and ygdB overlap by 4 nucleotides ygdB and ppdC overlap by 16 nucleotides ppdC and recC are separated by 12 nucleotides recC and ptrA are separated by 175 nucleotides
JDDGAC_23690: pulJ - prepilin peptidase-dependent protein, at 4,534,313 to 4,534,876
pulJ
JDDGAC_23695: ygdB - DUF2509 domain-containing protein, at 4,534,873 to 4,535,280
ygdB
JDDGAC_23700: ppdC - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 4,535,265 to 4,535,588
ppdC
JDDGAC_23705: recC - exodeoxyribonuclease V subunit gamma, at 4,535,601 to 4,538,969
recC
JDDGAC_23710: ptrA - pitrilysin, at 4,539,145 to 4,542,033
ptrA
Position (kb)
4535
4536
4537
4538
4539 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4534.699 kb on + strand, within pulJ at 4534.798 kb on + strand, within pulJ at 4534.799 kb on - strand, within pulJ at 4534.801 kb on + strand, within pulJ at 4534.801 kb on + strand, within pulJ at 4534.802 kb on - strand, within pulJ at 4534.816 kb on + strand, within pulJ at 4534.816 kb on + strand, within pulJ at 4534.941 kb on - strand, within ygdB at 4534.941 kb on - strand, within ygdB at 4535.087 kb on + strand, within ygdB at 4535.092 kb on + strand, within ygdB at 4535.092 kb on + strand, within ygdB at 4535.150 kb on + strand, within ygdB at 4535.150 kb on + strand, within ygdB at 4535.150 kb on + strand, within ygdB at 4535.150 kb on + strand, within ygdB at 4535.151 kb on - strand, within ygdB at 4535.161 kb on + strand, within ygdB at 4535.161 kb on + strand, within ygdB at 4535.375 kb on - strand, within ppdC at 4535.387 kb on + strand, within ppdC at 4535.527 kb on + strand, within ppdC at 4538.958 kb on - strand at 4539.038 kb on - strand at 4539.038 kb on - strand at 4539.038 kb on - strand at 4539.039 kb on + strand at 4539.039 kb on + strand at 4539.039 kb on + strand at 4539.039 kb on + strand at 4539.040 kb on - strand at 4539.040 kb on - strand at 4539.040 kb on - strand at 4539.040 kb on - strand at 4539.040 kb on - strand at 4539.040 kb on - strand at 4539.040 kb on - strand at 4539.041 kb on + strand at 4539.041 kb on + strand at 4539.041 kb on + strand at 4539.041 kb on + strand at 4539.042 kb on - strand at 4539.042 kb on - strand at 4539.047 kb on + strand at 4539.048 kb on - strand at 4539.072 kb on + strand at 4539.173 kb on + strand at 4539.173 kb on + strand at 4539.174 kb on - strand at 4539.174 kb on - strand at 4539.174 kb on - strand at 4539.182 kb on + strand at 4539.183 kb on - strand at 4539.183 kb on - strand at 4539.183 kb on - strand at 4539.183 kb on - strand at 4539.203 kb on + strand at 4539.250 kb on - strand at 4539.275 kb on - strand at 4539.283 kb on - strand at 4539.283 kb on - strand at 4539.285 kb on - strand at 4539.409 kb on + strand at 4539.409 kb on + strand at 4539.420 kb on + strand at 4539.420 kb on + strand at 4539.420 kb on + strand at 4539.421 kb on - strand at 4539.472 kb on + strand, within ptrA at 4539.617 kb on - strand, within ptrA at 4539.633 kb on + strand, within ptrA at 4539.633 kb on + strand, within ptrA at 4539.634 kb on - strand, within ptrA at 4539.654 kb on - strand, within ptrA at 4539.846 kb on + strand, within ptrA at 4539.856 kb on - strand, within ptrA at 4539.859 kb on - strand, within ptrA at 4539.951 kb on - strand, within ptrA
Per-strain Table
Position Strand Gene LocusTag Fraction GB_JK32 remove 4,534,699 + pulJ JDDGAC_23690 0.68 +0.1 4,534,798 + pulJ JDDGAC_23690 0.86 -1.2 4,534,799 - pulJ JDDGAC_23690 0.86 -0.8 4,534,801 + pulJ JDDGAC_23690 0.87 +1.2 4,534,801 + pulJ JDDGAC_23690 0.87 +1.1 4,534,802 - pulJ JDDGAC_23690 0.87 -0.1 4,534,816 + pulJ JDDGAC_23690 0.89 -0.9 4,534,816 + pulJ JDDGAC_23690 0.89 +1.7 4,534,941 - ygdB JDDGAC_23695 0.17 -0.8 4,534,941 - ygdB JDDGAC_23695 0.17 -1.8 4,535,087 + ygdB JDDGAC_23695 0.52 -0.5 4,535,092 + ygdB JDDGAC_23695 0.54 -0.5 4,535,092 + ygdB JDDGAC_23695 0.54 -0.6 4,535,150 + ygdB JDDGAC_23695 0.68 -0.5 4,535,150 + ygdB JDDGAC_23695 0.68 +0.8 4,535,150 + ygdB JDDGAC_23695 0.68 +2.3 4,535,150 + ygdB JDDGAC_23695 0.68 +1.4 4,535,151 - ygdB JDDGAC_23695 0.68 -0.7 4,535,161 + ygdB JDDGAC_23695 0.71 -2.2 4,535,161 + ygdB JDDGAC_23695 0.71 +0.6 4,535,375 - ppdC JDDGAC_23700 0.34 -2.2 4,535,387 + ppdC JDDGAC_23700 0.38 +0.8 4,535,527 + ppdC JDDGAC_23700 0.81 +1.0 4,538,958 - +0.7 4,539,038 - -0.4 4,539,038 - +0.5 4,539,038 - +1.9 4,539,039 + +0.9 4,539,039 + -0.5 4,539,039 + +0.0 4,539,039 + +0.4 4,539,040 - -0.1 4,539,040 - -0.2 4,539,040 - +1.7 4,539,040 - +0.5 4,539,040 - -2.5 4,539,040 - -0.7 4,539,040 - -0.4 4,539,041 + +0.7 4,539,041 + +0.8 4,539,041 + -0.4 4,539,041 + -2.2 4,539,042 - +0.2 4,539,042 - -0.5 4,539,047 + +0.4 4,539,048 - -0.6 4,539,072 + +0.9 4,539,173 + +1.1 4,539,173 + +0.0 4,539,174 - +0.6 4,539,174 - -1.6 4,539,174 - +0.9 4,539,182 + +0.0 4,539,183 - -3.3 4,539,183 - -1.5 4,539,183 - -0.1 4,539,183 - +0.1 4,539,203 + -1.4 4,539,250 - +1.2 4,539,275 - -1.4 4,539,283 - -2.5 4,539,283 - -2.0 4,539,285 - -2.1 4,539,409 + -0.4 4,539,409 + -1.6 4,539,420 + +1.0 4,539,420 + +1.0 4,539,420 + +0.3 4,539,421 - +0.9 4,539,472 + ptrA JDDGAC_23710 0.11 -1.8 4,539,617 - ptrA JDDGAC_23710 0.16 -0.1 4,539,633 + ptrA JDDGAC_23710 0.17 +0.1 4,539,633 + ptrA JDDGAC_23710 0.17 +0.6 4,539,634 - ptrA JDDGAC_23710 0.17 +0.9 4,539,654 - ptrA JDDGAC_23710 0.18 +0.2 4,539,846 + ptrA JDDGAC_23710 0.24 -2.1 4,539,856 - ptrA JDDGAC_23710 0.25 +1.7 4,539,859 - ptrA JDDGAC_23710 0.25 -1.0 4,539,951 - ptrA JDDGAC_23710 0.28 +0.5
Or see this region's nucleotide sequence