Experiment: GB_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt JDDGAC_23460 and JDDGAC_23465 are separated by 13 nucleotides JDDGAC_23465 and sctI are separated by 19 nucleotides sctI and escJ overlap by 4 nucleotides escJ and JDDGAC_23480 are separated by 15 nucleotides
JDDGAC_23460: JDDGAC_23460 - Type III secretion protein EprH, at 4,496,717 to 4,497,451
_23460
JDDGAC_23465: JDDGAC_23465 - type III secretion system needle complex protein, at 4,497,465 to 4,497,704
_23465
JDDGAC_23470: sctI - type III secretion system inner rod subunit SctI, at 4,497,724 to 4,498,056
sctI
JDDGAC_23475: escJ - EscJ/YscJ/HrcJ family type III secretion inner membrane ring protein, at 4,498,053 to 4,498,787
escJ
JDDGAC_23480: JDDGAC_23480 - type III secretion apparatus protein OrgA/MxiK, at 4,498,803 to 4,499,384
_23480
Position (kb) 4497
4498
4499 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4497.117 kb on + strand, within JDDGAC_23460 at 4497.427 kb on + strand at 4497.484 kb on - strand at 4497.868 kb on + strand, within sctI at 4497.975 kb on + strand, within sctI at 4498.059 kb on + strand at 4498.176 kb on - strand, within escJ at 4498.695 kb on - strand, within escJ at 4499.002 kb on + strand, within JDDGAC_23480
Per-strain Table
Position Strand Gene LocusTag Fraction GB_JK32 remove 4,497,117 + JDDGAC_23460 0.54 -0.6 4,497,427 + -1.6 4,497,484 - -0.9 4,497,868 + sctI JDDGAC_23470 0.43 -1.5 4,497,975 + sctI JDDGAC_23470 0.75 -2.0 4,498,059 + +2.3 4,498,176 - escJ JDDGAC_23475 0.17 +1.3 4,498,695 - escJ JDDGAC_23475 0.87 +1.8 4,499,002 + JDDGAC_23480 0.34 -0.7
Or see this region's nucleotide sequence