Experiment: GB_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt escD and sepL are separated by 142 nucleotides sepL and espA are separated by 58 nucleotides espA and espD are separated by 12 nucleotides
JDDGAC_19035: escD - type III secretion system LEE inner membrane ring protein EscD, at 3,621,815 to 3,623,035
escD
JDDGAC_19040: sepL - type III secretion system LEE gatekeeper SepL, at 3,623,178 to 3,624,233
sepL
JDDGAC_19045: espA - type III secretion system LEE translocon filament protein EspA, at 3,624,292 to 3,624,870
espA
JDDGAC_19050: espD - type III secretion system LEE translocon pore-forming subunit EspD, at 3,624,883 to 3,626,007
espD
Position (kb)
3623
3624
3625 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3622.568 kb on + strand, within escD at 3622.569 kb on - strand, within escD at 3622.800 kb on - strand, within escD at 3622.819 kb on - strand, within escD at 3622.883 kb on + strand, within escD at 3622.884 kb on - strand, within escD at 3622.884 kb on - strand, within escD at 3622.939 kb on + strand at 3622.952 kb on + strand at 3623.164 kb on + strand at 3623.334 kb on - strand, within sepL at 3623.526 kb on - strand, within sepL at 3624.285 kb on + strand at 3624.289 kb on + strand at 3624.289 kb on + strand at 3624.297 kb on + strand at 3624.356 kb on - strand, within espA at 3624.363 kb on - strand, within espA at 3624.448 kb on + strand, within espA at 3624.505 kb on - strand, within espA at 3624.622 kb on - strand, within espA at 3624.623 kb on + strand, within espA at 3624.636 kb on + strand, within espA at 3624.636 kb on + strand, within espA at 3624.637 kb on - strand, within espA at 3624.645 kb on - strand, within espA at 3624.787 kb on - strand, within espA at 3624.837 kb on + strand at 3624.864 kb on + strand at 3624.878 kb on + strand at 3624.882 kb on + strand at 3624.883 kb on - strand at 3624.883 kb on - strand at 3624.883 kb on - strand at 3624.894 kb on - strand at 3624.897 kb on + strand at 3624.931 kb on - strand at 3624.943 kb on - strand at 3624.996 kb on + strand, within espD at 3624.996 kb on + strand, within espD at 3625.067 kb on + strand, within espD at 3625.067 kb on + strand, within espD at 3625.098 kb on + strand, within espD at 3625.099 kb on - strand, within espD at 3625.099 kb on - strand, within espD at 3625.102 kb on - strand, within espD at 3625.102 kb on - strand, within espD at 3625.131 kb on + strand, within espD at 3625.131 kb on + strand, within espD at 3625.172 kb on - strand, within espD at 3625.172 kb on - strand, within espD at 3625.172 kb on - strand, within espD at 3625.177 kb on + strand, within espD at 3625.177 kb on + strand, within espD at 3625.177 kb on + strand, within espD at 3625.178 kb on - strand, within espD at 3625.178 kb on - strand, within espD
Per-strain Table
Position Strand Gene LocusTag Fraction GB_JK32 remove 3,622,568 + escD JDDGAC_19035 0.62 -0.5 3,622,569 - escD JDDGAC_19035 0.62 -2.5 3,622,800 - escD JDDGAC_19035 0.81 -0.1 3,622,819 - escD JDDGAC_19035 0.82 -1.8 3,622,883 + escD JDDGAC_19035 0.87 -1.6 3,622,884 - escD JDDGAC_19035 0.88 -0.9 3,622,884 - escD JDDGAC_19035 0.88 -0.7 3,622,939 + -2.0 3,622,952 + -2.0 3,623,164 + -1.8 3,623,334 - sepL JDDGAC_19040 0.15 -2.0 3,623,526 - sepL JDDGAC_19040 0.33 -2.4 3,624,285 + +0.2 3,624,289 + -0.1 3,624,289 + +1.2 3,624,297 + -1.3 3,624,356 - espA JDDGAC_19045 0.11 +1.4 3,624,363 - espA JDDGAC_19045 0.12 -0.1 3,624,448 + espA JDDGAC_19045 0.27 -0.7 3,624,505 - espA JDDGAC_19045 0.37 -0.7 3,624,622 - espA JDDGAC_19045 0.57 -0.0 3,624,623 + espA JDDGAC_19045 0.57 -2.5 3,624,636 + espA JDDGAC_19045 0.59 -3.2 3,624,636 + espA JDDGAC_19045 0.59 -1.8 3,624,637 - espA JDDGAC_19045 0.60 -0.4 3,624,645 - espA JDDGAC_19045 0.61 +2.2 3,624,787 - espA JDDGAC_19045 0.85 -0.3 3,624,837 + -1.0 3,624,864 + +1.4 3,624,878 + -0.5 3,624,882 + -1.0 3,624,883 - -1.4 3,624,883 - -0.5 3,624,883 - -0.7 3,624,894 - +0.3 3,624,897 + -0.2 3,624,931 - +1.6 3,624,943 - +0.0 3,624,996 + espD JDDGAC_19050 0.10 -1.6 3,624,996 + espD JDDGAC_19050 0.10 +0.7 3,625,067 + espD JDDGAC_19050 0.16 +0.4 3,625,067 + espD JDDGAC_19050 0.16 +0.1 3,625,098 + espD JDDGAC_19050 0.19 -1.0 3,625,099 - espD JDDGAC_19050 0.19 +0.9 3,625,099 - espD JDDGAC_19050 0.19 -0.1 3,625,102 - espD JDDGAC_19050 0.19 -0.5 3,625,102 - espD JDDGAC_19050 0.19 +1.2 3,625,131 + espD JDDGAC_19050 0.22 -1.4 3,625,131 + espD JDDGAC_19050 0.22 -1.6 3,625,172 - espD JDDGAC_19050 0.26 -1.2 3,625,172 - espD JDDGAC_19050 0.26 -0.8 3,625,172 - espD JDDGAC_19050 0.26 -0.2 3,625,177 + espD JDDGAC_19050 0.26 +2.4 3,625,177 + espD JDDGAC_19050 0.26 +2.1 3,625,177 + espD JDDGAC_19050 0.26 +0.3 3,625,178 - espD JDDGAC_19050 0.26 +1.3 3,625,178 - espD JDDGAC_19050 0.26 +1.6
Or see this region's nucleotide sequence