Strain Fitness in Escherichia coli ECRC98 around JDDGAC_13730

Experiment: GB_JK32

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaccA and dnaE are separated by 12 nucleotidesdnaE and rnhB are separated by 36 nucleotidesrnhB and lpxB overlap by 4 nucleotides JDDGAC_13725: accA - acetyl-CoA carboxylase carboxyl transferase subunit alpha, at 2,533,060 to 2,534,019 accA JDDGAC_13730: dnaE - DNA polymerase III subunit alpha, at 2,534,032 to 2,537,514 dnaE JDDGAC_13735: rnhB - ribonuclease HII, at 2,537,551 to 2,538,147 rnhB JDDGAC_13740: lpxB - lipid-A-disaccharide synthase, at 2,538,144 to 2,539,292 lpxB Position (kb) 2534 2535 2536 2537 2538Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2533.061 kb on + strandat 2533.074 kb on - strandat 2537.821 kb on - strand, within rnhBat 2537.838 kb on - strand, within rnhBat 2537.838 kb on - strand, within rnhBat 2537.838 kb on - strand, within rnhBat 2537.851 kb on - strand, within rnhBat 2537.897 kb on - strand, within rnhB

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Per-strain Table

Position Strand Gene LocusTag Fraction GB_JK32
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2,533,061 + -1.1
2,533,074 - -1.6
2,537,821 - rnhB JDDGAC_13735 0.45 -0.0
2,537,838 - rnhB JDDGAC_13735 0.48 -1.4
2,537,838 - rnhB JDDGAC_13735 0.48 +0.5
2,537,838 - rnhB JDDGAC_13735 0.48 +0.4
2,537,851 - rnhB JDDGAC_13735 0.50 -2.6
2,537,897 - rnhB JDDGAC_13735 0.58 +2.9

Or see this region's nucleotide sequence