Experiment: GB_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt JDDGAC_07035 and JDDGAC_07040 are separated by 33 nucleotides JDDGAC_07040 and eCs1768 are separated by 31 nucleotides eCs1768 and JDDGAC_07050 are separated by 68 nucleotides JDDGAC_07050 and ydaS overlap by 17 nucleotides ydaS and JDDGAC_07060 are separated by 124 nucleotides
JDDGAC_07035: JDDGAC_07035 - DUF1627 domain-containing protein, at 1,216,608 to 1,217,324
_07035
JDDGAC_07040: JDDGAC_07040 - replication protein, at 1,217,358 to 1,217,780
_07040
JDDGAC_07045: eCs1768 - phage replisome organizer, at 1,217,812 to 1,218,849
eCs1768
JDDGAC_07050: JDDGAC_07050 - Rha family transcriptional regulator, at 1,218,918 to 1,219,343
_07050
JDDGAC_07055: ydaS - transcriptional regulator, at 1,219,327 to 1,219,650
ydaS
JDDGAC_07060: JDDGAC_07060 - Prophage repressor RacR, at 1,219,775 to 1,220,251
_07060
Position (kb) 1217
1218
1219 Strain fitness (log2 ratio) -2
-1
0
1 at 1216.971 kb on - strand, within JDDGAC_07035 at 1217.389 kb on + strand at 1217.469 kb on - strand, within JDDGAC_07040 at 1218.186 kb on + strand, within eCs1768 at 1218.355 kb on - strand, within eCs1768 at 1219.216 kb on + strand, within JDDGAC_07050 at 1219.435 kb on + strand, within ydaS at 1219.435 kb on + strand, within ydaS at 1219.439 kb on + strand, within ydaS at 1219.534 kb on + strand, within ydaS at 1219.570 kb on + strand, within ydaS at 1219.634 kb on + strand at 1219.635 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction GB_JK32 remove 1,216,971 - JDDGAC_07035 0.51 +0.5 1,217,389 + +0.1 1,217,469 - JDDGAC_07040 0.26 +1.0 1,218,186 + eCs1768 JDDGAC_07045 0.36 +1.2 1,218,355 - eCs1768 JDDGAC_07045 0.52 -1.5 1,219,216 + JDDGAC_07050 0.70 +1.0 1,219,435 + ydaS JDDGAC_07055 0.33 +1.5 1,219,435 + ydaS JDDGAC_07055 0.33 +0.6 1,219,439 + ydaS JDDGAC_07055 0.35 +0.5 1,219,534 + ydaS JDDGAC_07055 0.64 -0.4 1,219,570 + ydaS JDDGAC_07055 0.75 -1.5 1,219,634 + -1.8 1,219,635 - +0.8
Or see this region's nucleotide sequence