Strain Fitness in Escherichia coli ECRC98 around JDDGAC_02195

Experiment: GB_JK32

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthdhA and uidR are separated by 240 nucleotidesuidR and lacZ are separated by 390 nucleotides JDDGAC_02190: hdhA - 7-alpha-hydroxysteroid dehydrogenase, at 392,884 to 393,651 hdhA JDDGAC_02195: uidR - DNA-binding transcriptional regulator UidR, at 393,892 to 394,470 uidR JDDGAC_02200: lacZ - Beta-galactosidase/beta-glucuronidase, at 394,861 to 395,574 lacZ Position (kb) 393 394 395Strain fitness (log2 ratio) -2 -1 0 1 2 3at 393.039 kb on - strand, within hdhAat 393.552 kb on + strand, within hdhAat 393.652 kb on + strandat 393.678 kb on + strandat 393.679 kb on - strandat 393.766 kb on + strandat 393.784 kb on - strandat 393.868 kb on + strandat 393.868 kb on + strandat 393.868 kb on + strandat 393.868 kb on + strandat 393.993 kb on + strand, within uidRat 394.135 kb on + strand, within uidRat 394.369 kb on + strand, within uidRat 394.370 kb on - strand, within uidRat 394.370 kb on - strand, within uidRat 394.376 kb on + strand, within uidRat 394.681 kb on - strandat 394.690 kb on + strandat 394.690 kb on + strandat 394.691 kb on - strandat 395.043 kb on + strand, within lacZat 395.247 kb on + strand, within lacZat 395.451 kb on + strand, within lacZ

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Per-strain Table

Position Strand Gene LocusTag Fraction GB_JK32
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393,039 - hdhA JDDGAC_02190 0.20 -0.1
393,552 + hdhA JDDGAC_02190 0.87 +1.9
393,652 + -0.1
393,678 + +1.3
393,679 - -0.9
393,766 + +0.8
393,784 - +2.3
393,868 + -1.5
393,868 + -0.6
393,868 + +2.0
393,868 + +0.6
393,993 + uidR JDDGAC_02195 0.17 -0.4
394,135 + uidR JDDGAC_02195 0.42 +0.2
394,369 + uidR JDDGAC_02195 0.82 -1.9
394,370 - uidR JDDGAC_02195 0.83 +1.0
394,370 - uidR JDDGAC_02195 0.83 +1.2
394,376 + uidR JDDGAC_02195 0.84 -0.7
394,681 - -1.0
394,690 + +1.0
394,690 + -2.3
394,691 - -1.2
395,043 + lacZ JDDGAC_02200 0.25 +2.8
395,247 + lacZ JDDGAC_02200 0.54 +0.0
395,451 + lacZ JDDGAC_02200 0.83 -0.7

Or see this region's nucleotide sequence