Experiment: GB_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cspE and rlmA are separated by 165 nucleotides rlmA and mntP overlap by 4 nucleotides mntP and JDDGAC_01085 overlap by 4 nucleotides JDDGAC_01085 and JDDGAC_01090 are separated by 310 nucleotides JDDGAC_01090 and manZ are separated by 54 nucleotides
JDDGAC_01070: cspE - transcription antiterminator/RNA stability regulator CspE, at 179,974 to 180,183
cspE
JDDGAC_01075: rlmA - 23S rRNA (guanine(745)-N(1))-methyltransferase, at 180,349 to 181,158
rlmA
JDDGAC_01080: mntP - manganese efflux pump MntP, at 181,155 to 181,721
mntP
JDDGAC_01085: JDDGAC_01085 - Membrane protein, at 181,718 to 181,840
_01085
JDDGAC_01090: JDDGAC_01090 - UPF0266 membrane protein YobD, at 182,151 to 182,609
_01090
JDDGAC_01095: manZ - PTS mannose transporter subunit IID, at 182,664 to 183,515
manZ
Position (kb) 181
182 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 180.171 kb on - strand at 180.171 kb on - strand at 180.177 kb on + strand at 180.177 kb on + strand at 180.177 kb on + strand at 180.177 kb on + strand at 180.178 kb on - strand at 180.210 kb on + strand at 180.210 kb on + strand at 180.258 kb on + strand at 180.329 kb on - strand at 180.331 kb on + strand at 180.403 kb on + strand at 180.403 kb on + strand at 180.403 kb on + strand at 180.404 kb on - strand at 180.404 kb on - strand at 180.406 kb on - strand at 180.406 kb on - strand at 180.406 kb on - strand at 180.474 kb on + strand, within rlmA at 180.475 kb on - strand, within rlmA at 180.533 kb on + strand, within rlmA at 180.533 kb on + strand, within rlmA at 180.806 kb on + strand, within rlmA at 180.806 kb on + strand, within rlmA at 180.806 kb on + strand, within rlmA at 180.810 kb on + strand, within rlmA at 180.810 kb on + strand, within rlmA at 180.811 kb on - strand, within rlmA at 180.839 kb on + strand, within rlmA at 181.153 kb on + strand at 181.239 kb on - strand, within mntP at 181.283 kb on + strand, within mntP at 181.284 kb on - strand, within mntP at 181.716 kb on - strand at 181.748 kb on - strand, within JDDGAC_01085 at 181.780 kb on - strand, within JDDGAC_01085 at 181.780 kb on - strand, within JDDGAC_01085 at 181.780 kb on - strand, within JDDGAC_01085 at 181.873 kb on + strand at 181.909 kb on - strand at 182.363 kb on - strand, within JDDGAC_01090 at 182.403 kb on + strand, within JDDGAC_01090 at 182.434 kb on + strand, within JDDGAC_01090 at 182.548 kb on + strand, within JDDGAC_01090 at 182.654 kb on + strand at 182.654 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction GB_JK32 remove 180,171 - -1.4 180,171 - +1.9 180,177 + +0.5 180,177 + +2.4 180,177 + -0.1 180,177 + +1.3 180,178 - +1.1 180,210 + -0.9 180,210 + -1.1 180,258 + +0.1 180,329 - -1.3 180,331 + +0.7 180,403 + -1.4 180,403 + +0.7 180,403 + +1.7 180,404 - -1.0 180,404 - +0.4 180,406 - +0.3 180,406 - -1.1 180,406 - -0.1 180,474 + rlmA JDDGAC_01075 0.15 +1.1 180,475 - rlmA JDDGAC_01075 0.16 +0.5 180,533 + rlmA JDDGAC_01075 0.23 -0.3 180,533 + rlmA JDDGAC_01075 0.23 -0.4 180,806 + rlmA JDDGAC_01075 0.56 +2.3 180,806 + rlmA JDDGAC_01075 0.56 -0.3 180,806 + rlmA JDDGAC_01075 0.56 -1.3 180,810 + rlmA JDDGAC_01075 0.57 +2.4 180,810 + rlmA JDDGAC_01075 0.57 +2.1 180,811 - rlmA JDDGAC_01075 0.57 +1.4 180,839 + rlmA JDDGAC_01075 0.60 -0.3 181,153 + -1.3 181,239 - mntP JDDGAC_01080 0.15 -0.2 181,283 + mntP JDDGAC_01080 0.23 -2.2 181,284 - mntP JDDGAC_01080 0.23 -1.0 181,716 - -1.5 181,748 - JDDGAC_01085 0.24 -1.7 181,780 - JDDGAC_01085 0.50 -0.7 181,780 - JDDGAC_01085 0.50 -1.5 181,780 - JDDGAC_01085 0.50 +3.0 181,873 + -1.4 181,909 - -1.5 182,363 - JDDGAC_01090 0.46 +1.7 182,403 + JDDGAC_01090 0.55 +0.7 182,434 + JDDGAC_01090 0.62 -0.2 182,548 + JDDGAC_01090 0.86 +2.1 182,654 + -0.1 182,654 + +0.0
Or see this region's nucleotide sequence