Experiment: GB_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tccP and espJ are separated by 324 nucleotides espJ and JDDGAC_00115 are separated by 207 nucleotides JDDGAC_00115 and JDDGAC_00120 are separated by 50 nucleotides
JDDGAC_00105: tccP - Tir-cytoskeleton coupling protein TccP, at 18,667 to 19,680
tccP
JDDGAC_00110: espJ - type III secretion system effector ADP-ribosyltransferase EspJ, at 20,005 to 20,658
espJ
JDDGAC_00115: JDDGAC_00115 - hypothetical protein, at 20,866 to 21,084
_00115
JDDGAC_00120: JDDGAC_00120 - tRNA-Xxx, at 21,135 to 21,224
_00120
Position (kb) 20
21 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 19.365 kb on + strand, within tccP at 19.715 kb on + strand at 19.723 kb on + strand at 19.723 kb on + strand at 19.813 kb on + strand at 19.904 kb on + strand at 19.967 kb on + strand at 20.024 kb on - strand at 20.279 kb on - strand, within espJ at 20.340 kb on - strand, within espJ at 20.730 kb on + strand at 21.011 kb on - strand, within JDDGAC_00115 at 21.134 kb on - strand at 21.136 kb on + strand at 21.136 kb on + strand at 21.157 kb on + strand, within JDDGAC_00120 at 21.163 kb on - strand, within JDDGAC_00120 at 21.395 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction GB_JK32 remove 19,365 + tccP JDDGAC_00105 0.69 -0.3 19,715 + +0.1 19,723 + +3.6 19,723 + +1.0 19,813 + -0.1 19,904 + -1.1 19,967 + +1.9 20,024 - -1.1 20,279 - espJ JDDGAC_00110 0.42 -1.9 20,340 - espJ JDDGAC_00110 0.51 -0.6 20,730 + -0.2 21,011 - JDDGAC_00115 0.66 +1.0 21,134 - +3.1 21,136 + +0.7 21,136 + -0.7 21,157 + JDDGAC_00120 0.24 -1.3 21,163 - JDDGAC_00120 0.31 +0.2 21,395 - -1.1
Or see this region's nucleotide sequence