Strain Fitness in Escherichia coli ECOR27 around NOLOHH_20895

Experiment: Bas38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nticd and rluE are separated by 171 nucleotidesrluE and nudJ are separated by 9 nucleotidesnudJ and mnmA are separated by 53 nucleotides NOLOHH_20885: icd - NADP-dependent isocitrate dehydrogenase, at 4,107,587 to 4,108,837 icd NOLOHH_20890: rluE - 23S rRNA pseudouridine(2457) synthase RluE, at 4,109,009 to 4,109,662 rluE NOLOHH_20895: nudJ - phosphatase NudJ, at 4,109,672 to 4,110,133 nudJ NOLOHH_20900: mnmA - tRNA 2-thiouridine(34) synthase MnmA, at 4,110,187 to 4,111,293 mnmA Position (kb) 4109 4110 4111Strain fitness (log2 ratio) -2 -1 0 1 2at 4108.889 kb on - strandat 4109.005 kb on + strandat 4109.005 kb on + strandat 4109.006 kb on - strandat 4109.007 kb on + strandat 4109.104 kb on + strand, within rluEat 4109.333 kb on + strand, within rluEat 4109.336 kb on + strand, within rluEat 4109.337 kb on - strand, within rluEat 4109.337 kb on - strand, within rluEat 4109.337 kb on - strand, within rluEat 4109.400 kb on + strand, within rluEat 4109.494 kb on + strand, within rluEat 4109.523 kb on + strand, within rluEat 4109.523 kb on + strand, within rluEat 4109.524 kb on - strand, within rluEat 4109.524 kb on - strand, within rluEat 4109.524 kb on - strand, within rluEat 4109.525 kb on + strand, within rluEat 4109.525 kb on + strand, within rluEat 4109.526 kb on - strand, within rluEat 4109.536 kb on + strand, within rluEat 4109.660 kb on + strandat 4109.943 kb on + strand, within nudJat 4110.131 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas38
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4,108,889 - -2.0
4,109,005 + +0.3
4,109,005 + +1.0
4,109,006 - +0.1
4,109,007 + +0.5
4,109,104 + rluE NOLOHH_20890 0.15 +1.8
4,109,333 + rluE NOLOHH_20890 0.50 -0.8
4,109,336 + rluE NOLOHH_20890 0.50 -1.1
4,109,337 - rluE NOLOHH_20890 0.50 +0.4
4,109,337 - rluE NOLOHH_20890 0.50 +0.3
4,109,337 - rluE NOLOHH_20890 0.50 +0.6
4,109,400 + rluE NOLOHH_20890 0.60 +1.6
4,109,494 + rluE NOLOHH_20890 0.74 -0.9
4,109,523 + rluE NOLOHH_20890 0.79 +1.3
4,109,523 + rluE NOLOHH_20890 0.79 +0.2
4,109,524 - rluE NOLOHH_20890 0.79 -0.1
4,109,524 - rluE NOLOHH_20890 0.79 +1.4
4,109,524 - rluE NOLOHH_20890 0.79 -0.4
4,109,525 + rluE NOLOHH_20890 0.79 -0.2
4,109,525 + rluE NOLOHH_20890 0.79 +0.6
4,109,526 - rluE NOLOHH_20890 0.79 +1.9
4,109,536 + rluE NOLOHH_20890 0.81 -0.2
4,109,660 + -1.1
4,109,943 + nudJ NOLOHH_20895 0.59 -2.1
4,110,131 + +0.8

Or see this region's nucleotide sequence