Strain Fitness in Escherichia coli ECOR27 around NOLOHH_20115

Experiment: Bas38

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Bas38
3,959,277	+	puuB	NOLOHH_20110	0.74	+0.5
3,959,378	+	puuB	NOLOHH_20110	0.82	-0.3
3,959,379	-	puuB	NOLOHH_20110	0.82	+1.1
3,959,561	+				+0.3
3,959,585	+				+1.0
3,959,586	-				+0.5
3,959,596	-				-0.5
3,959,919	-	puuC	NOLOHH_20115	0.21	+0.6
3,959,919	-	puuC	NOLOHH_20115	0.21	-0.7
3,959,937	-	puuC	NOLOHH_20115	0.22	-0.9
3,960,035	-	puuC	NOLOHH_20115	0.29	-1.3
3,960,141	-	puuC	NOLOHH_20115	0.36	-0.8
3,960,211	-	puuC	NOLOHH_20115	0.40	-2.6
3,960,211	-	puuC	NOLOHH_20115	0.40	-0.0
3,960,819	-	puuC	NOLOHH_20115	0.81	-1.7
3,961,013	-				-0.3
3,961,013	-				-0.3
3,961,125	-				+0.2
3,961,127	-				-1.6
3,961,127	-				+0.7
3,961,127	-				-0.3
3,961,127	-				-0.8
3,961,127	-				+0.4
3,961,202	-				+0.0
3,961,322	+				+0.0
3,961,323	-				+1.1
3,961,325	-				+1.7
3,961,356	+				-1.7
3,961,557	-	puuR	NOLOHH_20120	0.33	-0.4
3,961,753	+	puuR	NOLOHH_20120	0.68	-0.8
3,961,753	+	puuR	NOLOHH_20120	0.68	-1.6
3,961,754	-	puuR	NOLOHH_20120	0.69	-0.8
3,961,779	-	puuR	NOLOHH_20120	0.73	-0.1
3,961,779	-	puuR	NOLOHH_20120	0.73	+0.0
3,961,800	-	puuR	NOLOHH_20120	0.77	+0.5
3,961,810	-	puuR	NOLOHH_20120	0.79	+1.4
3,961,821	-	puuR	NOLOHH_20120	0.81	-0.2
3,961,929	-				-1.0
3,961,950	-				+0.7
3,961,979	-				+0.3
3,962,016	-				-0.8
3,962,016	-				+3.3