Experiment: Bas38
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insQ and yeaQ are separated by 226 nucleotides yeaQ and yoaJ are separated by 58 nucleotides yoaJ and dgcP are separated by 133 nucleotides dgcP and yoaF are separated by 182 nucleotides yoaF and NOLOHH_17195 overlap by 47 nucleotides NOLOHH_17195 and yeaO are separated by 2 nucleotides yeaO and nimT are separated by 54 nucleotides
NOLOHH_17170: insQ - Putative RNA-guided DNA endonuclease InsQ, at 3,371,292 to 3,372,440
insQ
NOLOHH_17175: yeaQ - UPF0410 protein YeaQ, at 3,372,667 to 3,372,915
yeaQ
NOLOHH_17180: yoaJ - protein YoaJ, at 3,372,974 to 3,373,048
yoaJ
NOLOHH_17185: dgcP - diguanylate cyclase DgcP, at 3,373,182 to 3,374,207
dgcP
NOLOHH_17190: yoaF - DUF333 domain-containing protein, at 3,374,390 to 3,374,644
yoaF
NOLOHH_17195: NOLOHH_17195 - Transposase, at 3,374,598 to 3,374,663
_17195
NOLOHH_17200: yeaO - Uncharacterized protein YeaO, at 3,374,666 to 3,375,013
yeaO
NOLOHH_17205: nimT - 2-nitroimidazole transporter, at 3,375,068 to 3,376,249
nimT
Position (kb)
3373
3374
3375 Strain fitness (log2 ratio)
-2
-1
0
1 at 3372.521 kb on + strand at 3372.654 kb on - strand at 3372.762 kb on + strand, within yeaQ at 3372.776 kb on + strand, within yeaQ at 3372.825 kb on + strand, within yeaQ at 3372.892 kb on - strand at 3372.920 kb on + strand at 3373.028 kb on - strand, within yoaJ at 3373.111 kb on - strand at 3373.111 kb on - strand at 3373.369 kb on - strand, within dgcP at 3373.369 kb on - strand, within dgcP at 3373.369 kb on - strand, within dgcP at 3373.369 kb on - strand, within dgcP at 3373.383 kb on - strand, within dgcP at 3373.533 kb on - strand, within dgcP at 3373.533 kb on - strand, within dgcP at 3373.749 kb on - strand, within dgcP at 3373.844 kb on - strand, within dgcP at 3374.048 kb on + strand, within dgcP at 3374.049 kb on - strand, within dgcP at 3374.052 kb on - strand, within dgcP at 3374.090 kb on - strand, within dgcP at 3374.366 kb on + strand at 3374.366 kb on + strand at 3374.467 kb on + strand, within yoaF at 3374.662 kb on - strand at 3374.674 kb on + strand at 3374.674 kb on + strand at 3374.674 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas38 remove 3,372,521 + -0.4 3,372,654 - +0.9 3,372,762 + yeaQ NOLOHH_17175 0.38 +0.4 3,372,776 + yeaQ NOLOHH_17175 0.44 -1.3 3,372,825 + yeaQ NOLOHH_17175 0.63 -0.2 3,372,892 - +1.1 3,372,920 + +0.3 3,373,028 - yoaJ NOLOHH_17180 0.72 -0.3 3,373,111 - +1.0 3,373,111 - +0.2 3,373,369 - dgcP NOLOHH_17185 0.18 -0.1 3,373,369 - dgcP NOLOHH_17185 0.18 -0.1 3,373,369 - dgcP NOLOHH_17185 0.18 -0.8 3,373,369 - dgcP NOLOHH_17185 0.18 -0.2 3,373,383 - dgcP NOLOHH_17185 0.20 +0.5 3,373,533 - dgcP NOLOHH_17185 0.34 -1.2 3,373,533 - dgcP NOLOHH_17185 0.34 -0.6 3,373,749 - dgcP NOLOHH_17185 0.55 -1.2 3,373,844 - dgcP NOLOHH_17185 0.65 +0.2 3,374,048 + dgcP NOLOHH_17185 0.84 +1.5 3,374,049 - dgcP NOLOHH_17185 0.85 -0.5 3,374,052 - dgcP NOLOHH_17185 0.85 -1.8 3,374,090 - dgcP NOLOHH_17185 0.88 -1.2 3,374,366 + -0.3 3,374,366 + -0.1 3,374,467 + yoaF NOLOHH_17190 0.30 -0.3 3,374,662 - +0.4 3,374,674 + -0.4 3,374,674 + -0.1 3,374,674 + -0.0
Or see this region's nucleotide sequence