Experiment: Bas38
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt iscS and iscU are separated by 27 nucleotides iscU and iscA are separated by 16 nucleotides iscA and hscB are separated by 95 nucleotides hscB and hscA are separated by 16 nucleotides
NOLOHH_13290: iscS - cysteine desulfurase, at 2,576,749 to 2,577,963
iscS
NOLOHH_13295: iscU - Iron-sulfur cluster assembly scaffold protein IscU, at 2,577,991 to 2,578,377
iscU
NOLOHH_13300: iscA - iron-sulfur cluster assembly protein IscA, at 2,578,394 to 2,578,717
iscA
NOLOHH_13305: hscB - co-chaperone HscB, at 2,578,813 to 2,579,328
hscB
NOLOHH_13310: hscA - Fe-S protein assembly chaperone HscA, at 2,579,345 to 2,581,195
hscA
Position (kb)
2578
2579 Strain fitness (log2 ratio)
-2
-1
0
1 at 2578.311 kb on + strand, within iscU at 2578.353 kb on + strand at 2578.353 kb on + strand at 2578.353 kb on + strand at 2578.393 kb on + strand at 2578.431 kb on + strand, within iscA at 2578.435 kb on + strand, within iscA at 2578.511 kb on + strand, within iscA at 2578.515 kb on + strand, within iscA at 2578.892 kb on + strand, within hscB at 2579.590 kb on + strand, within hscA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas38 remove 2,578,311 + iscU NOLOHH_13295 0.83 -0.6 2,578,353 + -0.7 2,578,353 + -1.3 2,578,353 + -0.4 2,578,393 + +0.1 2,578,431 + iscA NOLOHH_13300 0.11 +0.4 2,578,435 + iscA NOLOHH_13300 0.13 -1.8 2,578,511 + iscA NOLOHH_13300 0.36 -1.3 2,578,515 + iscA NOLOHH_13300 0.37 -0.3 2,578,892 + hscB NOLOHH_13305 0.15 -0.6 2,579,590 + hscA NOLOHH_13310 0.13 -0.1
Or see this region's nucleotide sequence