Experiment: Bas38
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Asp_trna and dnaQ are separated by 132 nucleotides dnaQ and rnhA are separated by 55 nucleotides rnhA and yafS overlap by 4 nucleotides yafS and gloB are separated by 33 nucleotides
NOLOHH_02305: Asp_trna - tRNA-Asp(gtc), at 371,930 to 372,006
_trna
NOLOHH_02310: dnaQ - DNA polymerase III subunit epsilon, at 372,139 to 372,879
dnaQ
NOLOHH_02315: rnhA - ribonuclease HI, at 372,935 to 373,402
rnhA
NOLOHH_02320: yafS - Uncharacterized protein YafS, at 373,399 to 374,121
yafS
NOLOHH_02325: gloB - hydroxyacylglutathione hydrolase, at 374,155 to 374,910
gloB
Position (kb) 372
373
374 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 372.039 kb on + strand at 372.882 kb on - strand at 372.882 kb on - strand at 372.901 kb on - strand at 372.901 kb on - strand at 372.917 kb on - strand at 372.941 kb on - strand at 372.941 kb on - strand at 372.941 kb on - strand at 373.007 kb on - strand, within rnhA at 373.012 kb on - strand, within rnhA at 373.012 kb on - strand, within rnhA at 373.091 kb on - strand, within rnhA at 373.151 kb on + strand, within rnhA at 373.152 kb on - strand, within rnhA at 373.152 kb on - strand, within rnhA at 373.165 kb on + strand, within rnhA at 373.166 kb on - strand, within rnhA at 373.183 kb on + strand, within rnhA at 373.184 kb on - strand, within rnhA at 373.400 kb on + strand at 373.413 kb on + strand at 373.414 kb on - strand at 373.465 kb on - strand at 373.518 kb on + strand, within yafS at 373.518 kb on + strand, within yafS at 373.518 kb on + strand, within yafS at 373.518 kb on + strand, within yafS at 373.519 kb on - strand, within yafS at 373.525 kb on + strand, within yafS at 373.525 kb on + strand, within yafS at 373.525 kb on + strand, within yafS at 373.525 kb on + strand, within yafS at 373.525 kb on + strand, within yafS at 373.526 kb on - strand, within yafS at 373.526 kb on - strand, within yafS at 373.545 kb on + strand, within yafS at 373.558 kb on + strand, within yafS at 373.660 kb on - strand, within yafS at 373.674 kb on + strand, within yafS at 373.674 kb on + strand, within yafS at 373.675 kb on - strand, within yafS at 373.676 kb on + strand, within yafS at 373.676 kb on + strand, within yafS at 373.677 kb on - strand, within yafS at 373.827 kb on + strand, within yafS at 373.827 kb on + strand, within yafS at 373.827 kb on + strand, within yafS at 373.827 kb on + strand, within yafS at 373.827 kb on + strand, within yafS at 373.827 kb on + strand, within yafS at 373.827 kb on + strand, within yafS at 373.827 kb on + strand, within yafS at 373.827 kb on + strand, within yafS at 373.827 kb on + strand, within yafS at 373.827 kb on + strand, within yafS at 373.828 kb on - strand, within yafS at 373.828 kb on - strand, within yafS at 373.828 kb on - strand, within yafS at 373.828 kb on - strand, within yafS at 373.828 kb on - strand, within yafS at 373.828 kb on - strand, within yafS at 373.828 kb on - strand, within yafS at 373.984 kb on - strand, within yafS at 374.142 kb on + strand at 374.142 kb on + strand at 374.143 kb on - strand at 374.149 kb on + strand at 374.150 kb on - strand at 374.150 kb on - strand at 374.150 kb on - strand at 374.154 kb on + strand at 374.154 kb on + strand at 374.155 kb on - strand at 374.169 kb on + strand at 374.170 kb on - strand at 374.170 kb on - strand at 374.170 kb on - strand at 374.191 kb on + strand at 374.191 kb on + strand at 374.191 kb on + strand at 374.191 kb on + strand at 374.191 kb on + strand at 374.191 kb on + strand at 374.191 kb on + strand at 374.191 kb on + strand at 374.192 kb on - strand at 374.192 kb on - strand at 374.261 kb on + strand, within gloB at 374.300 kb on + strand, within gloB at 374.300 kb on + strand, within gloB at 374.300 kb on + strand, within gloB at 374.301 kb on - strand, within gloB at 374.377 kb on + strand, within gloB at 374.377 kb on + strand, within gloB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas38 remove 372,039 + -0.4 372,882 - -1.3 372,882 - -0.0 372,901 - -0.9 372,901 - -0.5 372,917 - -0.3 372,941 - -1.1 372,941 - +1.0 372,941 - -0.0 373,007 - rnhA NOLOHH_02315 0.15 -0.7 373,012 - rnhA NOLOHH_02315 0.16 -0.6 373,012 - rnhA NOLOHH_02315 0.16 +0.1 373,091 - rnhA NOLOHH_02315 0.33 +0.3 373,151 + rnhA NOLOHH_02315 0.46 -1.9 373,152 - rnhA NOLOHH_02315 0.46 -0.3 373,152 - rnhA NOLOHH_02315 0.46 -0.0 373,165 + rnhA NOLOHH_02315 0.49 -3.0 373,166 - rnhA NOLOHH_02315 0.49 -0.7 373,183 + rnhA NOLOHH_02315 0.53 -2.1 373,184 - rnhA NOLOHH_02315 0.53 -2.5 373,400 + +1.2 373,413 + +0.4 373,414 - -0.2 373,465 - -0.0 373,518 + yafS NOLOHH_02320 0.16 +1.2 373,518 + yafS NOLOHH_02320 0.16 +0.3 373,518 + yafS NOLOHH_02320 0.16 -0.2 373,518 + yafS NOLOHH_02320 0.16 +0.1 373,519 - yafS NOLOHH_02320 0.17 -0.7 373,525 + yafS NOLOHH_02320 0.17 +0.3 373,525 + yafS NOLOHH_02320 0.17 -0.0 373,525 + yafS NOLOHH_02320 0.17 +0.8 373,525 + yafS NOLOHH_02320 0.17 -0.2 373,525 + yafS NOLOHH_02320 0.17 +0.5 373,526 - yafS NOLOHH_02320 0.18 +0.2 373,526 - yafS NOLOHH_02320 0.18 +0.1 373,545 + yafS NOLOHH_02320 0.20 -1.3 373,558 + yafS NOLOHH_02320 0.22 -0.1 373,660 - yafS NOLOHH_02320 0.36 +0.2 373,674 + yafS NOLOHH_02320 0.38 -0.1 373,674 + yafS NOLOHH_02320 0.38 +0.5 373,675 - yafS NOLOHH_02320 0.38 +0.1 373,676 + yafS NOLOHH_02320 0.38 -1.0 373,676 + yafS NOLOHH_02320 0.38 -0.8 373,677 - yafS NOLOHH_02320 0.38 -0.2 373,827 + yafS NOLOHH_02320 0.59 +0.2 373,827 + yafS NOLOHH_02320 0.59 -0.2 373,827 + yafS NOLOHH_02320 0.59 -0.1 373,827 + yafS NOLOHH_02320 0.59 +0.5 373,827 + yafS NOLOHH_02320 0.59 +0.4 373,827 + yafS NOLOHH_02320 0.59 +0.5 373,827 + yafS NOLOHH_02320 0.59 +0.4 373,827 + yafS NOLOHH_02320 0.59 +0.2 373,827 + yafS NOLOHH_02320 0.59 -0.2 373,827 + yafS NOLOHH_02320 0.59 +2.5 373,827 + yafS NOLOHH_02320 0.59 +0.0 373,828 - yafS NOLOHH_02320 0.59 -0.7 373,828 - yafS NOLOHH_02320 0.59 +0.1 373,828 - yafS NOLOHH_02320 0.59 -0.2 373,828 - yafS NOLOHH_02320 0.59 -0.8 373,828 - yafS NOLOHH_02320 0.59 +1.6 373,828 - yafS NOLOHH_02320 0.59 -0.3 373,828 - yafS NOLOHH_02320 0.59 +0.8 373,984 - yafS NOLOHH_02320 0.81 -1.2 374,142 + -1.3 374,142 + +0.7 374,143 - -0.4 374,149 + -0.8 374,150 - +0.9 374,150 - -0.3 374,150 - -0.7 374,154 + -1.1 374,154 + -0.6 374,155 - +0.3 374,169 + +0.1 374,170 - +0.5 374,170 - +0.5 374,170 - +0.6 374,191 + +0.6 374,191 + -1.0 374,191 + -0.6 374,191 + +1.6 374,191 + -0.6 374,191 + -0.2 374,191 + -0.0 374,191 + -0.0 374,192 - +0.9 374,192 - -0.7 374,261 + gloB NOLOHH_02325 0.14 +0.1 374,300 + gloB NOLOHH_02325 0.19 -0.6 374,300 + gloB NOLOHH_02325 0.19 -0.4 374,300 + gloB NOLOHH_02325 0.19 -0.2 374,301 - gloB NOLOHH_02325 0.19 +0.6 374,377 + gloB NOLOHH_02325 0.29 +0.0 374,377 + gloB NOLOHH_02325 0.29 +0.4
Or see this region's nucleotide sequence