Experiment: Bas37
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gdhA and ynjH are separated by 235 nucleotides ynjH and nudG overlap by 35 nucleotides nudG and ynjF are separated by 86 nucleotides ynjF and ynjE are separated by 8 nucleotides
NOLOHH_17355: gdhA - NADP-specific glutamate dehydrogenase, at 3,408,212 to 3,409,555
gdhA
NOLOHH_17360: ynjH - Uncharacterized protein YnjH, at 3,409,791 to 3,410,063
ynjH
NOLOHH_17365: nudG - CTP pyrophosphohydrolase, at 3,410,029 to 3,410,436
nudG
NOLOHH_17370: ynjF - Inner membrane protein YnjF, at 3,410,523 to 3,411,143
ynjF
NOLOHH_17375: ynjE - thiosulfate sulfurtransferase YnjE, at 3,411,152 to 3,412,459
ynjE
Position (kb)
3410
3411 Strain fitness (log2 ratio)
-2
-1
0
1 at 3409.145 kb on - strand, within gdhA at 3409.463 kb on + strand at 3409.464 kb on - strand at 3409.543 kb on - strand at 3409.819 kb on + strand, within ynjH at 3410.279 kb on - strand, within nudG at 3410.384 kb on - strand, within nudG at 3410.441 kb on + strand at 3410.505 kb on + strand at 3410.506 kb on - strand at 3410.507 kb on + strand at 3410.507 kb on + strand at 3410.614 kb on + strand, within ynjF at 3410.799 kb on + strand, within ynjF at 3410.799 kb on + strand, within ynjF at 3410.957 kb on + strand, within ynjF at 3410.957 kb on + strand, within ynjF at 3410.970 kb on + strand, within ynjF at 3411.007 kb on + strand, within ynjF at 3411.007 kb on + strand, within ynjF at 3411.160 kb on + strand at 3411.270 kb on + strand at 3411.274 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas37 remove 3,409,145 - gdhA NOLOHH_17355 0.69 -1.1 3,409,463 + +1.3 3,409,464 - -0.2 3,409,543 - +0.6 3,409,819 + ynjH NOLOHH_17360 0.10 +0.2 3,410,279 - nudG NOLOHH_17365 0.61 -1.7 3,410,384 - nudG NOLOHH_17365 0.87 -2.4 3,410,441 + -0.2 3,410,505 + -0.3 3,410,506 - -2.3 3,410,507 + -0.6 3,410,507 + +0.0 3,410,614 + ynjF NOLOHH_17370 0.15 +0.1 3,410,799 + ynjF NOLOHH_17370 0.44 +0.5 3,410,799 + ynjF NOLOHH_17370 0.44 -0.1 3,410,957 + ynjF NOLOHH_17370 0.70 -0.8 3,410,957 + ynjF NOLOHH_17370 0.70 +0.7 3,410,970 + ynjF NOLOHH_17370 0.72 +0.0 3,411,007 + ynjF NOLOHH_17370 0.78 +0.4 3,411,007 + ynjF NOLOHH_17370 0.78 -0.1 3,411,160 + +0.0 3,411,270 + -1.1 3,411,274 + +0.3
Or see this region's nucleotide sequence