Strain Fitness in Escherichia coli ECOR27 around NOLOHH_11515

Experiment: Bas37

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntprfB and lysS are separated by 9 nucleotideslysS and idi are separated by 42 nucleotidesidi and yqfH are separated by 54 nucleotidesyqfH and yqfG overlap by 4 nucleotidesyqfG and uacT are separated by 1 nucleotides NOLOHH_11510: prfB - peptide chain release factor 2, at 2,224,480 to 2,225,361 prfB NOLOHH_11515: lysS - lysine--tRNA ligase, at 2,225,371 to 2,226,888 lysS NOLOHH_11520: idi - isopentenyl-diphosphate Delta-isomerase, at 2,226,931 to 2,227,479 idi NOLOHH_11525: yqfH - Protein YqfH, at 2,227,534 to 2,227,605 yqfH NOLOHH_11530: yqfG - Uncharacterized protein YqfG, at 2,227,602 to 2,227,727 yqfG NOLOHH_11535: uacT - urate/proton symporter UacT, at 2,227,729 to 2,229,177 uacT Position (kb) 2225 2226 2227Strain fitness (log2 ratio) -1 0 1 2at 2225.502 kb on + strandat 2225.604 kb on + strand, within lysSat 2225.695 kb on + strand, within lysSat 2225.782 kb on - strand, within lysSat 2226.074 kb on - strand, within lysSat 2226.097 kb on + strand, within lysSat 2226.211 kb on + strand, within lysSat 2226.212 kb on - strand, within lysSat 2226.229 kb on + strand, within lysSat 2226.739 kb on + strandat 2226.740 kb on - strandat 2226.751 kb on + strandat 2226.887 kb on - strandat 2226.893 kb on + strandat 2226.893 kb on + strandat 2226.893 kb on + strandat 2226.893 kb on + strandat 2226.894 kb on - strandat 2226.894 kb on - strandat 2226.933 kb on - strandat 2226.933 kb on - strandat 2227.040 kb on - strand, within idiat 2227.040 kb on - strand, within idiat 2227.040 kb on - strand, within idiat 2227.040 kb on - strand, within idiat 2227.040 kb on - strand, within idiat 2227.040 kb on - strand, within idiat 2227.040 kb on - strand, within idiat 2227.040 kb on - strand, within idiat 2227.040 kb on - strand, within idiat 2227.049 kb on - strand, within idiat 2227.049 kb on - strand, within idiat 2227.094 kb on - strand, within idiat 2227.184 kb on + strand, within idiat 2227.189 kb on - strand, within idiat 2227.189 kb on - strand, within idiat 2227.189 kb on - strand, within idiat 2227.340 kb on - strand, within idiat 2227.340 kb on - strand, within idiat 2227.340 kb on - strand, within idiat 2227.342 kb on + strand, within idiat 2227.342 kb on + strand, within idiat 2227.343 kb on - strand, within idiat 2227.343 kb on - strand, within idiat 2227.456 kb on + strandat 2227.480 kb on - strandat 2227.494 kb on - strandat 2227.513 kb on + strandat 2227.516 kb on - strandat 2227.516 kb on - strandat 2227.520 kb on - strandat 2227.612 kb on - strandat 2227.620 kb on - strand, within yqfGat 2227.620 kb on - strand, within yqfGat 2227.622 kb on - strand, within yqfGat 2227.622 kb on - strand, within yqfGat 2227.622 kb on - strand, within yqfGat 2227.707 kb on - strand, within yqfGat 2227.728 kb on - strandat 2227.776 kb on + strandat 2227.776 kb on + strandat 2227.776 kb on + strandat 2227.777 kb on - strandat 2227.777 kb on - strandat 2227.777 kb on - strandat 2227.777 kb on - strandat 2227.777 kb on - strandat 2227.777 kb on - strandat 2227.777 kb on - strandat 2227.777 kb on - strandat 2227.777 kb on - strandat 2227.780 kb on + strandat 2227.780 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas37
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2,225,502 + -0.1
2,225,604 + lysS NOLOHH_11515 0.15 -0.0
2,225,695 + lysS NOLOHH_11515 0.21 +0.2
2,225,782 - lysS NOLOHH_11515 0.27 -0.6
2,226,074 - lysS NOLOHH_11515 0.46 -0.8
2,226,097 + lysS NOLOHH_11515 0.48 -0.3
2,226,211 + lysS NOLOHH_11515 0.55 -0.8
2,226,212 - lysS NOLOHH_11515 0.55 -1.0
2,226,229 + lysS NOLOHH_11515 0.57 +1.6
2,226,739 + -0.4
2,226,740 - -1.0
2,226,751 + +0.2
2,226,887 - -0.8
2,226,893 + -0.0
2,226,893 + +0.4
2,226,893 + -0.1
2,226,893 + +0.2
2,226,894 - -0.4
2,226,894 - +2.2
2,226,933 - +0.1
2,226,933 - +0.2
2,227,040 - idi NOLOHH_11520 0.20 +1.0
2,227,040 - idi NOLOHH_11520 0.20 -0.0
2,227,040 - idi NOLOHH_11520 0.20 +0.1
2,227,040 - idi NOLOHH_11520 0.20 +0.2
2,227,040 - idi NOLOHH_11520 0.20 +0.6
2,227,040 - idi NOLOHH_11520 0.20 +0.1
2,227,040 - idi NOLOHH_11520 0.20 +1.6
2,227,040 - idi NOLOHH_11520 0.20 -0.6
2,227,040 - idi NOLOHH_11520 0.20 -0.2
2,227,049 - idi NOLOHH_11520 0.21 +0.2
2,227,049 - idi NOLOHH_11520 0.21 -1.0
2,227,094 - idi NOLOHH_11520 0.30 -0.0
2,227,184 + idi NOLOHH_11520 0.46 +0.2
2,227,189 - idi NOLOHH_11520 0.47 +0.1
2,227,189 - idi NOLOHH_11520 0.47 -0.2
2,227,189 - idi NOLOHH_11520 0.47 +0.1
2,227,340 - idi NOLOHH_11520 0.74 +1.1
2,227,340 - idi NOLOHH_11520 0.74 +0.6
2,227,340 - idi NOLOHH_11520 0.74 -0.7
2,227,342 + idi NOLOHH_11520 0.75 +2.1
2,227,342 + idi NOLOHH_11520 0.75 +0.6
2,227,343 - idi NOLOHH_11520 0.75 +0.5
2,227,343 - idi NOLOHH_11520 0.75 -0.2
2,227,456 + -1.5
2,227,480 - -0.6
2,227,494 - +1.5
2,227,513 + -0.8
2,227,516 - +0.3
2,227,516 - +0.4
2,227,520 - +0.1
2,227,612 - +0.1
2,227,620 - yqfG NOLOHH_11530 0.14 +0.6
2,227,620 - yqfG NOLOHH_11530 0.14 -0.2
2,227,622 - yqfG NOLOHH_11530 0.16 -0.6
2,227,622 - yqfG NOLOHH_11530 0.16 -0.4
2,227,622 - yqfG NOLOHH_11530 0.16 -0.8
2,227,707 - yqfG NOLOHH_11530 0.83 +1.1
2,227,728 - +2.7
2,227,776 + -0.4
2,227,776 + -0.1
2,227,776 + +0.6
2,227,777 - +0.2
2,227,777 - -0.2
2,227,777 - -0.3
2,227,777 - -0.3
2,227,777 - -0.6
2,227,777 - -0.7
2,227,777 - +1.0
2,227,777 - +2.0
2,227,777 - +0.0
2,227,780 + +0.5
2,227,780 + +1.3

Or see this region's nucleotide sequence