Experiment: Bas37
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prfB and lysS are separated by 9 nucleotides lysS and idi are separated by 42 nucleotides idi and yqfH are separated by 54 nucleotides yqfH and yqfG overlap by 4 nucleotides yqfG and uacT are separated by 1 nucleotides
NOLOHH_11510: prfB - peptide chain release factor 2, at 2,224,480 to 2,225,361
prfB
NOLOHH_11515: lysS - lysine--tRNA ligase, at 2,225,371 to 2,226,888
lysS
NOLOHH_11520: idi - isopentenyl-diphosphate Delta-isomerase, at 2,226,931 to 2,227,479
idi
NOLOHH_11525: yqfH - Protein YqfH, at 2,227,534 to 2,227,605
yqfH
NOLOHH_11530: yqfG - Uncharacterized protein YqfG, at 2,227,602 to 2,227,727
yqfG
NOLOHH_11535: uacT - urate/proton symporter UacT, at 2,227,729 to 2,229,177
uacT
Position (kb)
2225
2226
2227 Strain fitness (log2 ratio)
-1
0
1
2 at 2225.502 kb on + strand at 2225.604 kb on + strand, within lysS at 2225.695 kb on + strand, within lysS at 2225.782 kb on - strand, within lysS at 2226.074 kb on - strand, within lysS at 2226.097 kb on + strand, within lysS at 2226.211 kb on + strand, within lysS at 2226.212 kb on - strand, within lysS at 2226.229 kb on + strand, within lysS at 2226.739 kb on + strand at 2226.740 kb on - strand at 2226.751 kb on + strand at 2226.887 kb on - strand at 2226.893 kb on + strand at 2226.893 kb on + strand at 2226.893 kb on + strand at 2226.893 kb on + strand at 2226.894 kb on - strand at 2226.894 kb on - strand at 2226.933 kb on - strand at 2226.933 kb on - strand at 2227.040 kb on - strand, within idi at 2227.040 kb on - strand, within idi at 2227.040 kb on - strand, within idi at 2227.040 kb on - strand, within idi at 2227.040 kb on - strand, within idi at 2227.040 kb on - strand, within idi at 2227.040 kb on - strand, within idi at 2227.040 kb on - strand, within idi at 2227.040 kb on - strand, within idi at 2227.049 kb on - strand, within idi at 2227.049 kb on - strand, within idi at 2227.094 kb on - strand, within idi at 2227.184 kb on + strand, within idi at 2227.189 kb on - strand, within idi at 2227.189 kb on - strand, within idi at 2227.189 kb on - strand, within idi at 2227.340 kb on - strand, within idi at 2227.340 kb on - strand, within idi at 2227.340 kb on - strand, within idi at 2227.342 kb on + strand, within idi at 2227.342 kb on + strand, within idi at 2227.343 kb on - strand, within idi at 2227.343 kb on - strand, within idi at 2227.456 kb on + strand at 2227.480 kb on - strand at 2227.494 kb on - strand at 2227.513 kb on + strand at 2227.516 kb on - strand at 2227.516 kb on - strand at 2227.520 kb on - strand at 2227.612 kb on - strand at 2227.620 kb on - strand, within yqfG at 2227.620 kb on - strand, within yqfG at 2227.622 kb on - strand, within yqfG at 2227.622 kb on - strand, within yqfG at 2227.622 kb on - strand, within yqfG at 2227.707 kb on - strand, within yqfG at 2227.728 kb on - strand at 2227.776 kb on + strand at 2227.776 kb on + strand at 2227.776 kb on + strand at 2227.777 kb on - strand at 2227.777 kb on - strand at 2227.777 kb on - strand at 2227.777 kb on - strand at 2227.777 kb on - strand at 2227.777 kb on - strand at 2227.777 kb on - strand at 2227.777 kb on - strand at 2227.777 kb on - strand at 2227.780 kb on + strand at 2227.780 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas37 remove 2,225,502 + -0.1 2,225,604 + lysS NOLOHH_11515 0.15 -0.0 2,225,695 + lysS NOLOHH_11515 0.21 +0.2 2,225,782 - lysS NOLOHH_11515 0.27 -0.6 2,226,074 - lysS NOLOHH_11515 0.46 -0.8 2,226,097 + lysS NOLOHH_11515 0.48 -0.3 2,226,211 + lysS NOLOHH_11515 0.55 -0.8 2,226,212 - lysS NOLOHH_11515 0.55 -1.0 2,226,229 + lysS NOLOHH_11515 0.57 +1.6 2,226,739 + -0.4 2,226,740 - -1.0 2,226,751 + +0.2 2,226,887 - -0.8 2,226,893 + -0.0 2,226,893 + +0.4 2,226,893 + -0.1 2,226,893 + +0.2 2,226,894 - -0.4 2,226,894 - +2.2 2,226,933 - +0.1 2,226,933 - +0.2 2,227,040 - idi NOLOHH_11520 0.20 +1.0 2,227,040 - idi NOLOHH_11520 0.20 -0.0 2,227,040 - idi NOLOHH_11520 0.20 +0.1 2,227,040 - idi NOLOHH_11520 0.20 +0.2 2,227,040 - idi NOLOHH_11520 0.20 +0.6 2,227,040 - idi NOLOHH_11520 0.20 +0.1 2,227,040 - idi NOLOHH_11520 0.20 +1.6 2,227,040 - idi NOLOHH_11520 0.20 -0.6 2,227,040 - idi NOLOHH_11520 0.20 -0.2 2,227,049 - idi NOLOHH_11520 0.21 +0.2 2,227,049 - idi NOLOHH_11520 0.21 -1.0 2,227,094 - idi NOLOHH_11520 0.30 -0.0 2,227,184 + idi NOLOHH_11520 0.46 +0.2 2,227,189 - idi NOLOHH_11520 0.47 +0.1 2,227,189 - idi NOLOHH_11520 0.47 -0.2 2,227,189 - idi NOLOHH_11520 0.47 +0.1 2,227,340 - idi NOLOHH_11520 0.74 +1.1 2,227,340 - idi NOLOHH_11520 0.74 +0.6 2,227,340 - idi NOLOHH_11520 0.74 -0.7 2,227,342 + idi NOLOHH_11520 0.75 +2.1 2,227,342 + idi NOLOHH_11520 0.75 +0.6 2,227,343 - idi NOLOHH_11520 0.75 +0.5 2,227,343 - idi NOLOHH_11520 0.75 -0.2 2,227,456 + -1.5 2,227,480 - -0.6 2,227,494 - +1.5 2,227,513 + -0.8 2,227,516 - +0.3 2,227,516 - +0.4 2,227,520 - +0.1 2,227,612 - +0.1 2,227,620 - yqfG NOLOHH_11530 0.14 +0.6 2,227,620 - yqfG NOLOHH_11530 0.14 -0.2 2,227,622 - yqfG NOLOHH_11530 0.16 -0.6 2,227,622 - yqfG NOLOHH_11530 0.16 -0.4 2,227,622 - yqfG NOLOHH_11530 0.16 -0.8 2,227,707 - yqfG NOLOHH_11530 0.83 +1.1 2,227,728 - +2.7 2,227,776 + -0.4 2,227,776 + -0.1 2,227,776 + +0.6 2,227,777 - +0.2 2,227,777 - -0.2 2,227,777 - -0.3 2,227,777 - -0.3 2,227,777 - -0.6 2,227,777 - -0.7 2,227,777 - +1.0 2,227,777 - +2.0 2,227,777 - +0.0 2,227,780 + +0.5 2,227,780 + +1.3
Or see this region's nucleotide sequence