Strain Fitness in Escherichia coli ECOR27 around NOLOHH_07550

Experiment: Bas37

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwaaO and rfaY are separated by 25 nucleotidesrfaY and rfaJ are separated by 24 nucleotides NOLOHH_07545: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 1,434,763 to 1,435,770 waaO NOLOHH_07550: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 1,435,796 to 1,436,503 rfaY NOLOHH_07555: rfaJ - glucosyltransferase, at 1,436,528 to 1,437,541 rfaJ Position (kb) 1435 1436 1437Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1434.824 kb on + strandat 1434.848 kb on + strandat 1434.903 kb on + strand, within waaOat 1435.176 kb on + strand, within waaOat 1435.286 kb on + strand, within waaOat 1435.326 kb on + strand, within waaOat 1435.458 kb on + strand, within waaOat 1435.552 kb on + strand, within waaOat 1435.747 kb on + strandat 1435.802 kb on + strandat 1435.881 kb on + strand, within rfaYat 1435.972 kb on + strand, within rfaYat 1436.152 kb on + strand, within rfaYat 1436.166 kb on - strand, within rfaYat 1436.392 kb on + strand, within rfaYat 1436.430 kb on - strand, within rfaYat 1436.525 kb on - strandat 1436.525 kb on - strandat 1436.749 kb on - strand, within rfaJat 1437.136 kb on - strand, within rfaJat 1437.321 kb on + strand, within rfaJat 1437.328 kb on + strand, within rfaJ

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas37
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1,434,824 + -1.9
1,434,848 + -3.2
1,434,903 + waaO NOLOHH_07545 0.14 -2.3
1,435,176 + waaO NOLOHH_07545 0.41 -0.0
1,435,286 + waaO NOLOHH_07545 0.52 -3.8
1,435,326 + waaO NOLOHH_07545 0.56 -3.0
1,435,458 + waaO NOLOHH_07545 0.69 -2.8
1,435,552 + waaO NOLOHH_07545 0.78 -3.6
1,435,747 + -3.3
1,435,802 + +1.2
1,435,881 + rfaY NOLOHH_07550 0.12 -0.8
1,435,972 + rfaY NOLOHH_07550 0.25 -0.7
1,436,152 + rfaY NOLOHH_07550 0.50 -0.2
1,436,166 - rfaY NOLOHH_07550 0.52 -3.6
1,436,392 + rfaY NOLOHH_07550 0.84 +0.1
1,436,430 - rfaY NOLOHH_07550 0.90 -3.3
1,436,525 - -2.6
1,436,525 - -3.3
1,436,749 - rfaJ NOLOHH_07555 0.22 -2.7
1,437,136 - rfaJ NOLOHH_07555 0.60 -4.4
1,437,321 + rfaJ NOLOHH_07555 0.78 -3.1
1,437,328 + rfaJ NOLOHH_07555 0.79 -3.8

Or see this region's nucleotide sequence