Experiment: Bas37
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recG and trmH are separated by 5 nucleotides trmH and spoT are separated by 6 nucleotides spoT and rpoZ are separated by 18 nucleotides rpoZ and gmk are separated by 54 nucleotides
NOLOHH_07425: recG - ATP-dependent DNA helicase RecG, at 1,412,584 to 1,414,665
recG
NOLOHH_07430: trmH - tRNA (guanosine(18)-2'-O)-methyltransferase TrmH, at 1,414,671 to 1,415,360
trmH
NOLOHH_07435: spoT - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 1,415,367 to 1,417,475
spoT
NOLOHH_07440: rpoZ - DNA-directed RNA polymerase subunit omega, at 1,417,494 to 1,417,769
rpoZ
NOLOHH_07445: gmk - guanylate kinase, at 1,417,824 to 1,418,447
gmk
Position (kb)
1415
1416
1417
1418 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1414.449 kb on + strand, within recG at 1414.484 kb on + strand at 1414.499 kb on - strand at 1414.552 kb on + strand at 1414.552 kb on + strand at 1414.552 kb on + strand at 1414.552 kb on + strand at 1414.553 kb on - strand at 1414.559 kb on - strand at 1414.559 kb on - strand at 1414.559 kb on - strand at 1414.600 kb on - strand at 1414.602 kb on + strand at 1414.602 kb on + strand at 1414.602 kb on + strand at 1414.602 kb on + strand at 1414.602 kb on + strand at 1414.603 kb on - strand at 1414.603 kb on - strand at 1414.603 kb on - strand at 1414.603 kb on - strand at 1414.649 kb on - strand at 1414.649 kb on - strand at 1414.672 kb on + strand at 1414.686 kb on + strand at 1414.686 kb on + strand at 1414.686 kb on + strand at 1414.686 kb on + strand at 1414.687 kb on - strand at 1414.687 kb on - strand at 1414.687 kb on - strand at 1414.687 kb on - strand at 1414.687 kb on - strand at 1414.688 kb on + strand at 1414.689 kb on - strand at 1414.689 kb on - strand at 1414.689 kb on - strand at 1414.849 kb on + strand, within trmH at 1414.850 kb on - strand, within trmH at 1414.850 kb on - strand, within trmH at 1415.160 kb on - strand, within trmH at 1415.227 kb on - strand, within trmH at 1415.227 kb on - strand, within trmH at 1415.227 kb on - strand, within trmH at 1415.253 kb on - strand, within trmH at 1415.268 kb on - strand, within trmH at 1415.268 kb on - strand, within trmH at 1415.360 kb on + strand at 1415.361 kb on - strand at 1415.361 kb on - strand at 1415.361 kb on - strand at 1415.368 kb on + strand at 1415.368 kb on + strand at 1415.368 kb on + strand at 1415.426 kb on + strand at 1415.426 kb on + strand at 1415.427 kb on - strand at 1415.476 kb on + strand at 1415.517 kb on - strand at 1415.534 kb on + strand at 1415.535 kb on - strand at 1415.535 kb on - strand at 1415.535 kb on - strand at 1415.639 kb on + strand, within spoT at 1415.640 kb on - strand, within spoT at 1415.675 kb on + strand, within spoT at 1415.796 kb on - strand, within spoT at 1416.310 kb on + strand, within spoT at 1417.627 kb on - strand, within rpoZ at 1417.728 kb on - strand, within rpoZ at 1417.741 kb on - strand, within rpoZ at 1417.774 kb on - strand at 1417.774 kb on - strand at 1417.810 kb on + strand at 1418.461 kb on - strand at 1418.461 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas37 remove 1,414,449 + recG NOLOHH_07425 0.90 -1.9 1,414,484 + +0.7 1,414,499 - -1.9 1,414,552 + -0.5 1,414,552 + -1.0 1,414,552 + -0.5 1,414,552 + +0.7 1,414,553 - +0.1 1,414,559 - -0.4 1,414,559 - -1.0 1,414,559 - +0.2 1,414,600 - -1.6 1,414,602 + +0.0 1,414,602 + -0.2 1,414,602 + +0.2 1,414,602 + -0.2 1,414,602 + -2.5 1,414,603 - -1.0 1,414,603 - -2.9 1,414,603 - -0.0 1,414,603 - +0.0 1,414,649 - -0.5 1,414,649 - +0.4 1,414,672 + -2.4 1,414,686 + -1.2 1,414,686 + -1.9 1,414,686 + -1.3 1,414,686 + -0.5 1,414,687 - +1.7 1,414,687 - +0.7 1,414,687 - -0.1 1,414,687 - -0.6 1,414,687 - -0.3 1,414,688 + +0.3 1,414,689 - -0.4 1,414,689 - -1.3 1,414,689 - -0.9 1,414,849 + trmH NOLOHH_07430 0.26 -0.4 1,414,850 - trmH NOLOHH_07430 0.26 -0.3 1,414,850 - trmH NOLOHH_07430 0.26 -0.3 1,415,160 - trmH NOLOHH_07430 0.71 -0.2 1,415,227 - trmH NOLOHH_07430 0.81 +0.3 1,415,227 - trmH NOLOHH_07430 0.81 +0.5 1,415,227 - trmH NOLOHH_07430 0.81 +0.5 1,415,253 - trmH NOLOHH_07430 0.84 -0.8 1,415,268 - trmH NOLOHH_07430 0.87 +0.0 1,415,268 - trmH NOLOHH_07430 0.87 -1.4 1,415,360 + +0.9 1,415,361 - -0.5 1,415,361 - -0.2 1,415,361 - -0.2 1,415,368 + -0.6 1,415,368 + -0.8 1,415,368 + -0.2 1,415,426 + -1.6 1,415,426 + -1.4 1,415,427 - -0.1 1,415,476 + +0.7 1,415,517 - -0.9 1,415,534 + -0.4 1,415,535 - +0.3 1,415,535 - +0.5 1,415,535 - +1.1 1,415,639 + spoT NOLOHH_07435 0.13 -2.0 1,415,640 - spoT NOLOHH_07435 0.13 -1.4 1,415,675 + spoT NOLOHH_07435 0.15 -0.0 1,415,796 - spoT NOLOHH_07435 0.20 +0.6 1,416,310 + spoT NOLOHH_07435 0.45 -1.8 1,417,627 - rpoZ NOLOHH_07440 0.48 +0.2 1,417,728 - rpoZ NOLOHH_07440 0.85 +0.5 1,417,741 - rpoZ NOLOHH_07440 0.89 -0.4 1,417,774 - -0.3 1,417,774 - -2.0 1,417,810 + -0.7 1,418,461 - -1.7 1,418,461 - -0.8
Or see this region's nucleotide sequence