Strain Fitness in Escherichia coli ECOR27 around NOLOHH_00285

Experiment: Bas37

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNOLOHH_00270 and NOLOHH_00275 are separated by 229 nucleotidesNOLOHH_00275 and NOLOHH_00280 overlap by 4 nucleotidesNOLOHH_00280 and NOLOHH_00285 are separated by 61 nucleotidesNOLOHH_00285 and NOLOHH_00290 are separated by 13 nucleotides NOLOHH_00270: NOLOHH_00270 - Endonuclease, at 4,351 to 4,842 _00270 NOLOHH_00275: NOLOHH_00275 - Conjugal transfer protein, at 5,072 to 5,377 _00275 NOLOHH_00280: NOLOHH_00280 - hypothetical protein, at 5,374 to 5,622 _00280 NOLOHH_00285: NOLOHH_00285 - cag pathogenicity island Cag12 family protein, at 5,684 to 6,220 _00285 NOLOHH_00290: NOLOHH_00290 - conjugal transfer protein TraG, at 6,234 to 8,066 _00290 Position (kb) 5 6 7Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4.700 kb on + strand, within NOLOHH_00270at 4.700 kb on + strand, within NOLOHH_00270at 4.762 kb on - strand, within NOLOHH_00270at 4.895 kb on + strandat 4.899 kb on - strandat 4.914 kb on - strandat 4.931 kb on - strandat 4.940 kb on - strandat 4.944 kb on + strandat 4.948 kb on + strandat 4.950 kb on + strandat 4.994 kb on + strandat 4.994 kb on + strandat 4.995 kb on - strandat 5.114 kb on - strand, within NOLOHH_00275at 5.114 kb on - strand, within NOLOHH_00275at 5.262 kb on - strand, within NOLOHH_00275at 5.270 kb on - strand, within NOLOHH_00275at 5.283 kb on - strand, within NOLOHH_00275at 5.283 kb on - strand, within NOLOHH_00275at 5.330 kb on - strand, within NOLOHH_00275at 5.404 kb on - strand, within NOLOHH_00280at 5.659 kb on + strandat 5.659 kb on + strandat 5.660 kb on - strandat 5.723 kb on - strandat 5.847 kb on + strand, within NOLOHH_00285at 6.303 kb on - strandat 6.394 kb on + strandat 6.543 kb on - strand, within NOLOHH_00290at 6.551 kb on + strand, within NOLOHH_00290at 6.552 kb on - strand, within NOLOHH_00290at 6.582 kb on - strand, within NOLOHH_00290at 6.590 kb on + strand, within NOLOHH_00290at 6.598 kb on - strand, within NOLOHH_00290at 6.643 kb on + strand, within NOLOHH_00290at 6.643 kb on + strand, within NOLOHH_00290at 6.708 kb on - strand, within NOLOHH_00290at 6.791 kb on + strand, within NOLOHH_00290at 6.793 kb on + strand, within NOLOHH_00290at 6.985 kb on - strand, within NOLOHH_00290at 7.099 kb on + strand, within NOLOHH_00290at 7.170 kb on + strand, within NOLOHH_00290at 7.170 kb on + strand, within NOLOHH_00290

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas37
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4,700 + NOLOHH_00270 0.71 +0.8
4,700 + NOLOHH_00270 0.71 +0.2
4,762 - NOLOHH_00270 0.84 -0.0
4,895 + -0.1
4,899 - -0.3
4,914 - -0.9
4,931 - +0.6
4,940 - -2.3
4,944 + -0.2
4,948 + -0.4
4,950 + -0.1
4,994 + +0.2
4,994 + +0.6
4,995 - +0.3
5,114 - NOLOHH_00275 0.14 +0.2
5,114 - NOLOHH_00275 0.14 +0.8
5,262 - NOLOHH_00275 0.62 -0.0
5,270 - NOLOHH_00275 0.65 -0.1
5,283 - NOLOHH_00275 0.69 -0.4
5,283 - NOLOHH_00275 0.69 -0.2
5,330 - NOLOHH_00275 0.84 -0.2
5,404 - NOLOHH_00280 0.12 +0.1
5,659 + -0.4
5,659 + +0.1
5,660 - +0.3
5,723 - -3.8
5,847 + NOLOHH_00285 0.30 -0.8
6,303 - -0.4
6,394 + -0.0
6,543 - NOLOHH_00290 0.17 -0.9
6,551 + NOLOHH_00290 0.17 +0.4
6,552 - NOLOHH_00290 0.17 -0.8
6,582 - NOLOHH_00290 0.19 -1.0
6,590 + NOLOHH_00290 0.19 +0.2
6,598 - NOLOHH_00290 0.20 -2.2
6,643 + NOLOHH_00290 0.22 +0.5
6,643 + NOLOHH_00290 0.22 +0.1
6,708 - NOLOHH_00290 0.26 +0.5
6,791 + NOLOHH_00290 0.30 +0.2
6,793 + NOLOHH_00290 0.30 +0.2
6,985 - NOLOHH_00290 0.41 +0.3
7,099 + NOLOHH_00290 0.47 +0.1
7,170 + NOLOHH_00290 0.51 +0.8
7,170 + NOLOHH_00290 0.51 +0.5

Or see this region's nucleotide sequence