Experiment: Control_ECOR27
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purU and Tyr_trna are separated by 159 nucleotides Tyr_trna and Tyr_trna are separated by 34 nucleotides Tyr_trna and tpr are separated by 67 nucleotides tpr and narI are separated by 204 nucleotides narI and narJ overlap by 1 nucleotides narJ and narH overlap by 4 nucleotides
NOLOHH_20480: purU - formyltetrahydrofolate deformylase, at 4,030,980 to 4,031,822
purU
NOLOHH_20485: Tyr_trna - tRNA-Tyr(gta), at 4,031,982 to 4,032,066
_trna
NOLOHH_20490: Tyr_trna - tRNA-Tyr(gta), at 4,032,101 to 4,032,185
_trna
NOLOHH_20495: tpr - Protamine-like protein, at 4,032,253 to 4,032,342
tpr
NOLOHH_20500: narI - respiratory nitrate reductase subunit gamma, at 4,032,547 to 4,033,224
narI
NOLOHH_20505: narJ - nitrate reductase molybdenum cofactor assembly chaperone, at 4,033,224 to 4,033,934
narJ
NOLOHH_20510: narH - nitrate reductase subunit beta, at 4,033,931 to 4,035,469
narH
Position (kb) 4032
4033
4034 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4031.595 kb on + strand, within purU at 4031.596 kb on - strand, within purU at 4031.696 kb on + strand, within purU at 4031.700 kb on + strand, within purU at 4031.700 kb on + strand, within purU at 4031.714 kb on + strand, within purU at 4031.714 kb on + strand, within purU at 4031.715 kb on - strand, within purU at 4031.764 kb on + strand at 4031.773 kb on + strand at 4031.836 kb on + strand at 4031.898 kb on + strand at 4031.974 kb on + strand at 4032.017 kb on + strand, within Tyr_trna at 4032.074 kb on + strand at 4032.077 kb on + strand at 4032.077 kb on + strand at 4032.231 kb on + strand at 4032.436 kb on - strand at 4032.529 kb on - strand at 4032.572 kb on + strand at 4032.573 kb on - strand at 4032.577 kb on - strand at 4032.577 kb on - strand at 4032.577 kb on - strand at 4032.577 kb on - strand at 4032.577 kb on - strand at 4032.775 kb on - strand, within narI at 4032.996 kb on - strand, within narI at 4032.996 kb on - strand, within narI at 4032.996 kb on - strand, within narI at 4033.181 kb on - strand at 4033.185 kb on - strand at 4033.191 kb on - strand at 4033.195 kb on - strand at 4033.707 kb on + strand, within narJ at 4033.708 kb on - strand, within narJ at 4033.915 kb on - strand at 4034.036 kb on - strand at 4034.140 kb on - strand, within narH at 4034.140 kb on - strand, within narH at 4034.140 kb on - strand, within narH
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECOR27 remove 4,031,595 + purU NOLOHH_20480 0.73 +0.1 4,031,596 - purU NOLOHH_20480 0.73 +0.7 4,031,696 + purU NOLOHH_20480 0.85 -0.8 4,031,700 + purU NOLOHH_20480 0.85 +0.4 4,031,700 + purU NOLOHH_20480 0.85 +0.2 4,031,714 + purU NOLOHH_20480 0.87 +0.8 4,031,714 + purU NOLOHH_20480 0.87 +0.6 4,031,715 - purU NOLOHH_20480 0.87 +0.1 4,031,764 + +0.6 4,031,773 + +0.5 4,031,836 + +1.8 4,031,898 + -0.4 4,031,974 + -0.0 4,032,017 + Tyr_trna NOLOHH_20485 0.41 +0.3 4,032,074 + +1.0 4,032,077 + -1.5 4,032,077 + -0.4 4,032,231 + +0.3 4,032,436 - +3.2 4,032,529 - +0.1 4,032,572 + +0.2 4,032,573 - -1.5 4,032,577 - +0.7 4,032,577 - +0.2 4,032,577 - -0.2 4,032,577 - -2.9 4,032,577 - +0.0 4,032,775 - narI NOLOHH_20500 0.34 -0.2 4,032,996 - narI NOLOHH_20500 0.66 -1.8 4,032,996 - narI NOLOHH_20500 0.66 +0.5 4,032,996 - narI NOLOHH_20500 0.66 -0.8 4,033,181 - +0.2 4,033,185 - +1.3 4,033,191 - +0.5 4,033,195 - +0.2 4,033,707 + narJ NOLOHH_20505 0.68 +1.2 4,033,708 - narJ NOLOHH_20505 0.68 +0.6 4,033,915 - +0.5 4,034,036 - -0.6 4,034,140 - narH NOLOHH_20510 0.14 +1.3 4,034,140 - narH NOLOHH_20510 0.14 +0.4 4,034,140 - narH NOLOHH_20510 0.14 -0.6
Or see this region's nucleotide sequence