Strain Fitness in Escherichia coli ECOR27 around NOLOHH_19455

Experiment: Control_ECOR27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpaaJ and paaI overlap by 1 nucleotidespaaI and paaC overlap by 11 nucleotidespaaC and paaG are separated by 46 nucleotidespaaG and paaF overlap by 1 nucleotides NOLOHH_19445: paaJ - bifunctional 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase, at 3,835,014 to 3,836,219 paaJ NOLOHH_19450: paaI - hydroxyphenylacetyl-CoA thioesterase PaaI, at 3,836,219 to 3,836,641 paaI NOLOHH_19455: paaC - 3-hydroxyacyl-CoA dehydrogenase PaaC, at 3,836,631 to 3,838,013 paaC NOLOHH_19460: paaG - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase PaaG, at 3,838,060 to 3,838,848 paaG NOLOHH_19465: paaF - 2,3-dehydroadipyl-CoA hydratase, at 3,838,848 to 3,839,615 paaF Position (kb) 3836 3837 3838 3839Strain fitness (log2 ratio) -3 -2 -1 0 1at 3835.674 kb on - strand, within paaJat 3836.001 kb on + strand, within paaJat 3836.157 kb on + strandat 3836.313 kb on + strand, within paaIat 3836.314 kb on - strand, within paaIat 3836.343 kb on - strand, within paaIat 3836.360 kb on + strand, within paaIat 3836.361 kb on - strand, within paaIat 3836.475 kb on - strand, within paaIat 3836.633 kb on - strandat 3836.633 kb on - strandat 3836.704 kb on + strandat 3836.733 kb on + strandat 3836.734 kb on - strandat 3837.464 kb on - strand, within paaCat 3837.687 kb on - strand, within paaCat 3837.687 kb on - strand, within paaCat 3837.959 kb on - strandat 3838.051 kb on - strandat 3838.061 kb on + strandat 3838.061 kb on + strandat 3838.062 kb on - strandat 3838.062 kb on - strandat 3838.198 kb on - strand, within paaGat 3838.202 kb on - strand, within paaGat 3838.427 kb on - strand, within paaGat 3838.433 kb on - strand, within paaGat 3838.438 kb on - strand, within paaGat 3838.609 kb on + strand, within paaGat 3838.642 kb on - strand, within paaGat 3838.693 kb on + strand, within paaGat 3838.694 kb on - strand, within paaGat 3838.694 kb on - strand, within paaGat 3838.823 kb on - strandat 3838.823 kb on - strandat 3838.882 kb on - strandat 3838.936 kb on - strand, within paaFat 3839.011 kb on - strand, within paaFat 3839.011 kb on - strand, within paaFat 3839.011 kb on - strand, within paaF

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECOR27
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3,835,674 - paaJ NOLOHH_19445 0.55 +0.1
3,836,001 + paaJ NOLOHH_19445 0.82 -1.2
3,836,157 + -1.9
3,836,313 + paaI NOLOHH_19450 0.22 -0.6
3,836,314 - paaI NOLOHH_19450 0.22 -1.2
3,836,343 - paaI NOLOHH_19450 0.29 +0.9
3,836,360 + paaI NOLOHH_19450 0.33 -0.3
3,836,361 - paaI NOLOHH_19450 0.34 +1.3
3,836,475 - paaI NOLOHH_19450 0.61 -0.2
3,836,633 - -0.2
3,836,633 - -0.7
3,836,704 + -0.4
3,836,733 + +0.6
3,836,734 - +0.8
3,837,464 - paaC NOLOHH_19455 0.60 -0.5
3,837,687 - paaC NOLOHH_19455 0.76 -1.0
3,837,687 - paaC NOLOHH_19455 0.76 -1.6
3,837,959 - -0.2
3,838,051 - -2.2
3,838,061 + -0.5
3,838,061 + +0.6
3,838,062 - +1.3
3,838,062 - -0.4
3,838,198 - paaG NOLOHH_19460 0.17 -1.0
3,838,202 - paaG NOLOHH_19460 0.18 -3.1
3,838,427 - paaG NOLOHH_19460 0.47 -1.5
3,838,433 - paaG NOLOHH_19460 0.47 -0.4
3,838,438 - paaG NOLOHH_19460 0.48 +1.4
3,838,609 + paaG NOLOHH_19460 0.70 +1.5
3,838,642 - paaG NOLOHH_19460 0.74 +0.0
3,838,693 + paaG NOLOHH_19460 0.80 -0.3
3,838,694 - paaG NOLOHH_19460 0.80 +0.9
3,838,694 - paaG NOLOHH_19460 0.80 -0.3
3,838,823 - -0.7
3,838,823 - +0.5
3,838,882 - -0.3
3,838,936 - paaF NOLOHH_19465 0.11 -0.9
3,839,011 - paaF NOLOHH_19465 0.21 +0.1
3,839,011 - paaF NOLOHH_19465 0.21 +0.0
3,839,011 - paaF NOLOHH_19465 0.21 +1.1

Or see this region's nucleotide sequence