Experiment: Control_ECOR27
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt paaJ and paaI overlap by 1 nucleotides paaI and paaC overlap by 11 nucleotides paaC and paaG are separated by 46 nucleotides paaG and paaF overlap by 1 nucleotides
NOLOHH_19445: paaJ - bifunctional 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase, at 3,835,014 to 3,836,219
paaJ
NOLOHH_19450: paaI - hydroxyphenylacetyl-CoA thioesterase PaaI, at 3,836,219 to 3,836,641
paaI
NOLOHH_19455: paaC - 3-hydroxyacyl-CoA dehydrogenase PaaC, at 3,836,631 to 3,838,013
paaC
NOLOHH_19460: paaG - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase PaaG, at 3,838,060 to 3,838,848
paaG
NOLOHH_19465: paaF - 2,3-dehydroadipyl-CoA hydratase, at 3,838,848 to 3,839,615
paaF
Position (kb)
3836
3837
3838
3839 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3835.674 kb on - strand, within paaJ at 3836.001 kb on + strand, within paaJ at 3836.157 kb on + strand at 3836.313 kb on + strand, within paaI at 3836.314 kb on - strand, within paaI at 3836.343 kb on - strand, within paaI at 3836.360 kb on + strand, within paaI at 3836.361 kb on - strand, within paaI at 3836.475 kb on - strand, within paaI at 3836.633 kb on - strand at 3836.633 kb on - strand at 3836.704 kb on + strand at 3836.733 kb on + strand at 3836.734 kb on - strand at 3837.464 kb on - strand, within paaC at 3837.687 kb on - strand, within paaC at 3837.687 kb on - strand, within paaC at 3837.959 kb on - strand at 3838.051 kb on - strand at 3838.061 kb on + strand at 3838.061 kb on + strand at 3838.062 kb on - strand at 3838.062 kb on - strand at 3838.198 kb on - strand, within paaG at 3838.202 kb on - strand, within paaG at 3838.427 kb on - strand, within paaG at 3838.433 kb on - strand, within paaG at 3838.438 kb on - strand, within paaG at 3838.609 kb on + strand, within paaG at 3838.642 kb on - strand, within paaG at 3838.693 kb on + strand, within paaG at 3838.694 kb on - strand, within paaG at 3838.694 kb on - strand, within paaG at 3838.823 kb on - strand at 3838.823 kb on - strand at 3838.882 kb on - strand at 3838.936 kb on - strand, within paaF at 3839.011 kb on - strand, within paaF at 3839.011 kb on - strand, within paaF at 3839.011 kb on - strand, within paaF
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECOR27 remove 3,835,674 - paaJ NOLOHH_19445 0.55 +0.1 3,836,001 + paaJ NOLOHH_19445 0.82 -1.2 3,836,157 + -1.9 3,836,313 + paaI NOLOHH_19450 0.22 -0.6 3,836,314 - paaI NOLOHH_19450 0.22 -1.2 3,836,343 - paaI NOLOHH_19450 0.29 +0.9 3,836,360 + paaI NOLOHH_19450 0.33 -0.3 3,836,361 - paaI NOLOHH_19450 0.34 +1.3 3,836,475 - paaI NOLOHH_19450 0.61 -0.2 3,836,633 - -0.2 3,836,633 - -0.7 3,836,704 + -0.4 3,836,733 + +0.6 3,836,734 - +0.8 3,837,464 - paaC NOLOHH_19455 0.60 -0.5 3,837,687 - paaC NOLOHH_19455 0.76 -1.0 3,837,687 - paaC NOLOHH_19455 0.76 -1.6 3,837,959 - -0.2 3,838,051 - -2.2 3,838,061 + -0.5 3,838,061 + +0.6 3,838,062 - +1.3 3,838,062 - -0.4 3,838,198 - paaG NOLOHH_19460 0.17 -1.0 3,838,202 - paaG NOLOHH_19460 0.18 -3.1 3,838,427 - paaG NOLOHH_19460 0.47 -1.5 3,838,433 - paaG NOLOHH_19460 0.47 -0.4 3,838,438 - paaG NOLOHH_19460 0.48 +1.4 3,838,609 + paaG NOLOHH_19460 0.70 +1.5 3,838,642 - paaG NOLOHH_19460 0.74 +0.0 3,838,693 + paaG NOLOHH_19460 0.80 -0.3 3,838,694 - paaG NOLOHH_19460 0.80 +0.9 3,838,694 - paaG NOLOHH_19460 0.80 -0.3 3,838,823 - -0.7 3,838,823 - +0.5 3,838,882 - -0.3 3,838,936 - paaF NOLOHH_19465 0.11 -0.9 3,839,011 - paaF NOLOHH_19465 0.21 +0.1 3,839,011 - paaF NOLOHH_19465 0.21 +0.0 3,839,011 - paaF NOLOHH_19465 0.21 +1.1
Or see this region's nucleotide sequence